Measurement of circulating red cell volume using biotin‐labeled red cells: validation against<sup>51</sup>Cr‐labeled red cells

Mock, Donald M.; Lankford, Gary L.; Widness, John A.; Burmeister, Leon F.; Kahn, Daniel; Strauss, Ronald G.

doi:10.1046/j.1537-2995.1999.39299154728.x

Cited by 54 publications

(109 citation statements)

References 22 publications

Supporting

Mentioning

106

Contrasting

Order By: Relevance

“…1 Error in the biotin method: Our studies measuring circulating RBC volume in adult subjects provide evidence that the biotin method is accurate (16). Circulating RBC volume by biotin correlated strongly with 51 Cr (correlation coefficient ϭ 0.97).…”

Section: Discussionmentioning

confidence: 86%

See 1 more Smart Citation

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

et al. 2001

View full text Add to dashboard Cite

Although circulating red blood cell (RBC) volume is a better measure of total body oxygen delivering capacity than hematocrit (HCT), circulating RBC volume is more difficult to measure. Thus, the HCT is often used in RBC transfusion decisions. However, several previous studies of low birth weight infants have reported that the correlation between HCT and circulating RBC volume is poor. Using a robust nonradioactive method based on in vivo dilution of biotinylated RBC enumerated by flow cytometry, the present study reexamined the correlation between HCT and circulating RBC volume in very low birth weight infants. Venous and capillary HCT levels were compared with circulating RBC volume measured using the biotin method. Twenty-six stable very low birth weight infants with birth weights less than 1300 g were studied on 43 occasions between 7 and 79 d of life. Venous HCT values correlated highly with circulating RBC volume (r ϭ 0.907; p Ͻ 0.0001). However, the mean 95% confidence limits for prediction of circulating RBC volume from venous HCT (the average error of prediction) was Ϯ13.4 mL/kg. The correlation between HCT and circulating RBC volume is strong in older stable very low birth weight infants. However, clinically important uncertainty exists in estimating circulating RBC volume and the associated RBC transfusion needs of an individual infant based on venous HCT. Intensive care of critically ill newborn infants has increased blood sampling and exacerbated the severity of neonatal anemia. Daily phlebotomy blood losses of 4 to 5% of the total blood volume are common among VLBW infants (1-3) who are defined here as those infants with birth weight less than 1500 g. Not surprisingly, the majority of the multiple RBC transfusions such infants receive are administered during the first weeks of life when phlebotomy loss is greatest (1, 2, 4, 5). The present consensus is that phlebotomy loss from clinical monitoring is the primary cause of the relatively large transfusion needs of VLBW infants (1, 3, 6, 7).The decision to administer a RBC transfusion is typically made on the basis of the infant's clinical condition and the whole blood HCT or blood Hb concentration (3,6,8). Although circulating RBC volume is a more accurate indicator of total body oxygen delivery capacity than whole blood HCT or Hb concentration (9, 10), HCT and Hb concentration are used clinically because they are quick, inexpensive, and analytically reliable. To understand the limitations of HCT as a guide to transfusion decisions, the relationship between HCT and circulating RBC volume in VLBW infants should be elucidated, and the sources of the previously reported poor correlation (9 -14) should be segregated into methodologic artifacts and genuine characteristics of preterm physiology.The aim of the present study was to examine the relationship between HCT and circulating RBC volume in stable VLBW infants. An accurate nonradioactive method based on biotinylated RBC was used to measure circulating RBC volume. ABSTRACT 525

show abstract

Section: Discussionmentioning

confidence: 86%

“…This method has been thoroughly validated against 14 Ccyanate in sheep (15) and against 51 Cr in adult volunteers (16). The method has been scaled down for VLBW infants.…”

Section: Methodologic Detailmentioning

confidence: 99%

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Other cell markers (e.g. RhD, Duffy, Kell, MN antigens 8 -10 or biotin labelling 17 ) can then be used for estimating FPV at subsequent transfusions but the time-consuming analyses will reduce the application for clinical use. Secondly, a sample is often taken during the transfusion to see whether more blood is needed.…”

Section: (Equation 1) Differ Significantly From Those Worked Out Withmentioning

confidence: 99%

A new method to determine the feto-placental volume based on dilution of fetal haemoglobin and an estimation of plasma fluid loss after intrauterine intravascular transfusion

Hoogeveen¹

2002

BJOG: An International Journal of Obstetrics and Gynaecology

View full text Add to dashboard Cite

show abstract

“…Specifically, the posttransfusion increases in blood Hct levels, changes of pretransfusion versus post-transfusion blood chemistry results, and rates of transfusion reactions-the last being remarkably rare-were comparable whether stored or fresh allogeneic RBCs were transfused. 7 In the current report, the efficacy of transfusing stored allogeneic RBCs was further elucidated by measuring the 24-hour posttransfusion recovery (PTR 24 ) and subsequent intravascular survival of RBCs obtained from blood bank units, labeled with biotin 8,9 and then transfused into preterm infants for measurements of circulating kinetics.…”

mentioning

confidence: 99%

Posttransfusion 24‐hour recovery and subsequent survival of allogeneic red blood cells in the bloodstream of newborn infants

et al. 2004

Self Cite

View full text Add to dashboard Cite

show abstract

Measurement of circulating red cell volume using biotin‐labeled red cells: validation against⁵¹Cr‐labeled red cells

Abstract: Circulating red cell volume measured by the use of biotin with either 125I-streptavidin or flow cytometry agrees with that measured by 51Cr. Each system provides a method of performing these studies without exposing the subject to radiation.

Cited by 54 publications

References 22 publications

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

A new method to determine the feto-placental volume based on dilution of fetal haemoglobin and an estimation of plasma fluid loss after intrauterine intravascular transfusion

Posttransfusion 24‐hour recovery and subsequent survival of allogeneic red blood cells in the bloodstream of newborn infants

Contact Info

Product

Resources

About

Measurement of circulating red cell volume using biotin‐labeled red cells: validation against51Cr‐labeled red cells

Abstract: Circulating red cell volume measured by the use of biotin with either 125I-streptavidin or flow cytometry agrees with that measured by 51Cr. Each system provides a method of performing these studies without exposing the subject to radiation.

Cited by 54 publications

References 22 publications

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

Hematocrit Correlates Well with Circulating Red Blood Cell Volume in Very Low Birth Weight Infants

A new method to determine the feto-placental volume based on dilution of fetal haemoglobin and an estimation of plasma fluid loss after intrauterine intravascular transfusion

Posttransfusion 24‐hour recovery and subsequent survival of allogeneic red blood cells in the bloodstream of newborn infants

Contact Info

Product

Resources

About

Measurement of circulating red cell volume using biotin‐labeled red cells: validation against⁵¹Cr‐labeled red cells