Measurement of body composition of live rats by electromagnetic conductance

Baer, David J.; Rumpler, William V.; Barnes, Rhoda E.; Kressler, L.L; Howe, Juliette C.; Haines, Tom

doi:10.1016/0031-9384(93)90379-t

Cited by 19 publications

(5 citation statements)

References 9 publications

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“…This decrease in water content indicates a decrease in the hydration of FFM. Baer et al (1993) similarly found a decrease in the mean hydration of FFM (r = −0.59, P < 0.0001) with age (Baer et al, 1993). Therefore, as suggested by Pietrobeli et al, the overestimation of fat content may be explained by a decrease in the FFM hydration.…”

Section: Discussionmentioning

confidence: 58%

Chemical versus dual energy x-ray absorptiometry for detecting age-associated body compositional changes in male rats☆

Feely¹,

Larkin²,

Halter³

et al. 2000

Experimental Gerontology

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Aging is associated with increases in body mass and fat mass (FM), whereas fat-free mass (FFM) either decreases or remains unchanged. The purpose of this study was to determine whether dual-energy X-ray absorptiometry (DXA) accurately detects age-associated changes in male Fischer 344 x Brown-Norway rats ages 8, 18, and 28 months. Eviscerated animal carcasses were first examined via the Lunar DPX-IQ DXA (small animal software version 1.0; HiRes (0.6 x 1.2 mm) medium mode). Eviscerated carcasses were then weighed, autoclaved, homogenized, and fat isolated from aliquots of homogenate via methanol/chloroform extraction. In both chemical (CHEM) and DXA analysis, carcass mass (CM), FM, and % fat were significantly higher (P < 0.0001) in the 18 and 28 versus 8-month-old rats. CHEM showed greater FFM in the 18 versus 8 months-old rats but not the 28 months-old animals. DXA was unable to detect the age-associated changes in FFM. Regression analysis showed a strong correlation between CHEM and DXA methods for CM (r = 0.98, P < 0.0001) and FM (r = 0.97, P < 0.0001), but less strong for FFM (r = 0.59, P = 0.0002). In conclusion, compared to CHEM, DXA consistently overestimated CM and FM across the age groups by 9% and 77%, respectively, and underestimated FFM by 5%.

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Section: Discussionmentioning

confidence: 58%

Chemical versus dual energy x-ray absorptiometry for detecting age-associated body compositional changes in male rats☆

Feely¹,

Larkin²,

Halter³

et al. 2000

Experimental Gerontology

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“…A crucial consequence of this is the limited usefulness of derived calibration curves. Measurements carried out in the same manner in different experiments may fail to predict body chemical composition accurately, (Bear et al, 1993;Bellinger and Williams, 1993;Skagen et al, 1993;Bell et al, 1994;Trocki et al, 1995) and underlines the empirical nature of the TOBEC method. Factors responsible include the size, shape and dimensions of an individual in relation to the volume of the measurement chamber.…”

Section: Discussionmentioning

confidence: 91%

Evaluation of the non invasive tobec (total body electrical conductivity) procedure for prediction of chemical components of male broilers with special consideration of dietary protein level

Dänicke

Halle²,

Jeroch³

1997

Archiv für Tierernaehrung

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TOBEC (total body electrical conductivity) measurement as a non invasive procedure for the estimation of body chemical composition was used to calculate calibration curves for the prediction of crude protein mass (CPM), crude water mass (CWM), crude ash mass (CAM) and fat-free mass (FFM) of male broiler chickens. A growth experiment with 3 protein levels (130, 230 and 330 g CP/kg diet, isoenergetic with 13.3 MJ AMEN/kg) was combined with TOBEC measurements and body chemical analysis in order to obtain the values necessary for calibration. A total of 196 TOBEC measurements and body chemical analysis were undertaken in time intervals of two days beginning with batch until day 17 of age. Different dietary protein levels resulted in marked differences in body weights and body chemical compositions but in similar TOBEC-responses for a given mass of FFM, CPM, CAM or CWM. Values for birds fed a diet with 130 g CP/kg diet tended to be more variable. Linear broken relationships were found between FFM, CPM, CAM and CWM, respectively and TOBEC values (E#). A set of different regression equations is given and yielded high proportions of variance accounted for the piece wise regression model (R2 ranged from 0.83 to 0.99). In spite of these high determinations the prediction of crude fat mass (CFM) by subtracting the FFM from the body weight resulted in most cases in weak determinations between observed and predicted CFM (R2 ranged from 0.38 to 0.86). The highest R2 was observed when the E# was expressed per unit metabolic body weight to the power of 0.67 and regressed on FFM expressed to the same power. In conclusion, FFM, CPM and CWM may be predicted reasonably well by TOBEC. However, these high determinations are not high enough to predict CFM accurately. In addition, the application of such regressions to an individual bird seems to be impossible. Assessment for groups of animals should be possible if errors of estimation, standard deviations and differences to be detected are taken into account in the calculation of the number of birds necessary for the TOBEC measurements.

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“…Recent reports have questioned the reliability of total body electrical conductivity in the determination of fat mass and fat-free mass in Fischer F344 rats (30) and in Sprague-Dawley rats (31). However, others have compared direct carcass analysis to tetrapolar bioelectrical impedance analysis in female Sprague-Dawley rats (32), and to dual energy X-ray absorptiometry in male Fischer F344 rats (33), and have suggested that these two methods may be appropriate for the estimation of in vivo body composition in rats during periods of weight stability (32) and during aging (33).…”

Section: Discussionmentioning

confidence: 99%

Effect of Age on Protein Conservation during Very‐Low‐Energy Diet in Obese Sprague‐Dawley Rats

Johnson

Yang

et al. 1998

Obesity Research

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JOHNSON, JULIA A, CHOON-HIE YU, MEI-UlH YANG, F. XAVIER PI-SUNYER. Effect of age on protein conservation during very-low-energy diet in obese SpragueDawley rats. Obes. Res. 1998;6:448--457. Objective: To examine the effect of age on body protein losses occurring during severe energy restriction in obesity. Research Methods and Procedures: Weanling (young) Sprague-Dawley rats (YR) were fed a high fat (35% energy) diet (HFD) until mean body weight approached that of a group of chow-fed retired breeder (aged) rats (AR). Both groups were then fed HFD for an additional 2 weeks, after which selected controls from YR and AR groups were killed for baseline carcass analysis. Remaining rats were fed a very-low-energy diet (VLED, 33% kcal of HFD) for 3 weeks and then killed for carcass analysis. Results: YR had greater fat stores before VLED, and lost proportionately more fat and less protein during VLED than did AR. Weight loss composition during VLED was 66.7% fat, 11.1% protein, and 22.2% water in YR, and 39.4% fat, 26.2% protein, and 34.3% water in AR. Greater YR fat loss during VLE]j (70.6 ± 30.4 vs. 32.6 ± 29.1 gin AR; mean ± SD) was paralleled by significantly larger decreases in epididymal and retroperitoneal fat pad weights, mean adipocyte size, and lipoprotein lipase activity. Greater protein loss in AR (21.6 ± 13.9 g vs. 11.8 ± 10.7 g in YR) coincided with larger decreases in visceral organ weights and serum thyroxine and triiodothyronine. Energy expenditure changes during VLED were similar between groups. Discussion: Dietary obese young rats appear better able 448 OBESITY RESEARCH Vol. 6 No.6 Nov. 1998 than aged rats to conserve body protein while losing body fat during severe energy restriction.

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Measurement of body composition of live rats by electromagnetic conductance

Cited by 19 publications

References 9 publications

Chemical versus dual energy x-ray absorptiometry for detecting age-associated body compositional changes in male rats☆

Chemical versus dual energy x-ray absorptiometry for detecting age-associated body compositional changes in male rats☆

Evaluation of the non invasive tobec (total body electrical conductivity) procedure for prediction of chemical components of male broilers with special consideration of dietary protein level

Effect of Age on Protein Conservation during Very‐Low‐Energy Diet in Obese Sprague‐Dawley Rats

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