Measurement of biological parameters during fermentation processes

Scheper, Thomas; Gebauer, A.; Sauerbrei, A.; Niehoff, Anja; Schügerl, K.

doi:10.1016/s0003-2670(00)81499-3

Cited by 46 publications

(15 citation statements)

References 9 publications

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“…Most of these methods use (3-lactamase to hydrolyze the penicillin fol lowed by detection of the formed penicilloic acid by various methods: 1) reduc tion of molybdoarsenic acid to molybdenum blue, which can be measured spectrophotometrically [Schneider, 1984;Carlsen et al, 1993a]; 2) reduction of iodine to iodine ions and detection of the iodine consumption by measuring the decrease in the absorbance of an iodine-starch complex [van Opstal et al, 1990;Carlsen et al, 1993a]; 3) measurement of the pH change, either by use of pH-dependent colour indicators [Yerian et al, 1988;Fuh et al, 1988] or by a pH-electrode [Gnanasekaran and Mottola, 1985;Olsson, 1988;Carlsen et al, 1993a]; and 4) measurement of the reaction enthalpy of the enzymatic reaction by a thermistor [Decristoforo and Danielsson, 1984;Scheper et al, 1984;Rank et al, 1992]. There are also several reports on the integration of a biosensor for measuring penicillin into FIA systems.…”

Section: On-line Flow Injection Analysismentioning

confidence: 99%

Physiological Engineering

1997

Physiological Engineering Aspects of Penicillium Chrysogenum

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Section: On-line Flow Injection Analysismentioning

confidence: 99%

Physiological Engineering

1997

Physiological Engineering Aspects of Penicillium Chrysogenum

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“…As efficiency of the bioprocess is only accessible by a sophisticated process control, cultivation process data must be collected and analyzed in real time [1][2][3][4][5][6][7]. Moreover, stricter conditions of the Food and Drug Association (FDA) for pharmaceutical industry concerning process documentation imposed by the Process and Analytical Technology (PAT) initiative have increased the demand for inline sensors, which are able to monitor and document the production process very accurately.…”

Section: Introductionmentioning

confidence: 99%

A review of non-invasive optical-based image analysis systems for continuous bioprocess monitoring

Höpfner

Bluma

Rudolph

et al. 2009

Bioprocess Biosyst Eng

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To observe and control cultivation processes, optical sensors are used increasingly. Important variables for controlling such processes are cell count, cell size distribution and the morphology of cells. Among turbidity measurement methods, imaging procedures are applied for determining these process values. A disadvantage of most previously developed imaging procedures is that they are only available offline, which requires sampling. On the other hand, available imaging inline probes can only deliver a limited number of process values so far. This contribution gives an overview of optical procedures for the inline determination of cell count, cell size distribution and other variables. In particular, by in situ microscopy, an imaging procedure will be described, which allows the determination of direct and non-direct cell variables in real time without sampling.

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“…A major impetus for the development of this monitoring techniques is the process analytical technology (PAT) initiative of the FDA [10][11][12]. The monitoring of crystallization processes with noninvasive sensor technologies would help to improve the efficiency and stability of the downstreaming of biologically interesting products.…”

Section: Introductionmentioning

confidence: 99%