Measurement of Barley β-Glucan Concentration in the Plasma by Sandwich ELISA Using Rat Dectin-1

Abstract

We used recombinant rat dectin-1 proteins to newly establish sandwich ELISA for determining barleyglucan (BG). The ELISA method had a working range of 15-4,000 g/L. Plasma BG was detectable up to 24 h after an intravenous administration of BG (1 mg/kg of body weight) to rats. This method may be an effective tool for investigating the immune modulatory effects of BG.