Measurement of Antibodies to Varicella-Zoster Virus Using a Virus-Free Fluorescent-Antibody-to-Membrane-Antigen (FAMA) Test

Park, Rackhyun; Hwang, Ji Young; Lee, Kang Il; Namkoong, Sim; Choi, Seo‐Hyun; Park, Songyong; Park, Hosun; Park, Junsoo

doi:10.4014/jmb.1408.08048

Cited by 8 publications

(5 citation statements)

References 18 publications

(27 reference statements)

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“…The reasons that varicella serology results were not analyzed were that some patients have had natural varicella infection, some patients received one versus two varicella vaccine doses (the vaccination guidelines in Québec changed while the project was ongoing), and finally there was missing information about varicella immunization or natural infection. Also, fluorescent antibody to membrane antigen is considered as the gold standard for detection of VZV antibodies in immunized individuals, yet it is currently not routinely available in Canada and could not be used for this project.…”

Section: Discussionmentioning

confidence: 99%

Reevaluating immunization delays after red blood cell transfusion

et al. 2019

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BACKGROUND: Current American and Canadian guidelines recommend delaying live vaccine immunization by 5 to 6 months after transfusion of red blood cells (RBCs) due to potential interference by serum antibodies. Due to lack of data, the recommendations may be unfounded and prevent valuable vaccination opportunities for children with frequent blood transfusions. The primary aim of this study was to determine measles, mumps, rubella (MMR) vaccine immunogenicity in patients chronically transfused with RBCs. A secondary aim was to quantify vaccine antibodies in RBC donations. STUDY DESIGN AND METHODS: MMR-specificantibodies were quantified in 25 pediatric patients who received both doses of MMR vaccine while on a chronic RBC transfusion program (median, 6.6 years after vaccination). MMR antibodies were also quantified in 30 samples of supernatants from RBC donations. RESULTS:Immunity to each of the MMR components was 68% to 76%. In blood donors born before 1976 (pre-MMR vaccine), 67% of MMR serologies in the supernatants of RBC donations were at the immune level versus 7% in blood donors born after 1976.CONCLUSION: This is the first study of the impact of RBC transfusions on MMR vaccine immunogenicity. Although lower than in the literature (immunity rates, ≥90%), the results show a high rate of immunogenicity of the MMR vaccine in chronically transfused patients. Additionally, results suggest that MMR antibody content in transfusions will continue to decrease with time. Weighing the risks and benefits of disease prevention in a vulnerable population, a reevaluation of immunization delays after RBC transfusions is called for.

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Section: Discussionmentioning

confidence: 99%

Reevaluating immunization delays after red blood cell transfusion

et al. 2019

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“…To avoid the use of infectious viruses, one study has reported a simple and safer FAMA using HEK293T cells transfected with a plasmid encoding VZV glycoprotein E (gE) to replace VZV-infected cells, which is called the gE FAMA (schematic shown in Figure 1 C) [ 36 ]. The gE FAMA exhibited a similar staining effect to classic FAMA, and the gE-FAMA titers were closely correlated with the gp-ELISA data.…”

Section: Methods For the Detection Of Anti-vzv Antibodiesmentioning

confidence: 99%

Current Methods for the Detection of Antibodies of Varicella-Zoster Virus: A Review

2023

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Infection with the varicella-zoster virus (VZV) causes chickenpox and shingles, which lead to significant morbidity and mortality globally. The detection of serum VZV-specific antibodies is important for the clinical diagnosis and sero-epidemiological research of VZV infection, and for assessing the effect of VZV vaccine immunization. Over recent decades, a variety of methods for VZV antibody detection have been developed. This review summarizes and compares the current methods for detecting VZV antibodies, and discussed future directions for this field.

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“…Имеющиеся к настоящему времени тест системы для серодиагностики ВО основаны на при менении цельновирионного препарата ВВЗ или рекомбинантных аналогов гликопротеина E ВВЗ (GE) -одного из основных вирусных антигенов группы продуктов поздних генов, избирательно взаимодействующего со специфическими антите лами класса G при ВО и ОЛ [5]. В диагностических тестах применяется иммунодоминантный фрагмент белка, не содержащий эпитопов, перекрестнореа гирующих с родственными герпесвирусами [6].…”

Section: Introductionunclassified

Development of the ELISA Test System for Quantitative Determination of IgG to the Varizella zoster virus in Human Serum and Assessment of its Diagnostic Efficiency

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Нестеренко

et al. 2022

Epidemiology and Vaccinal Prevention

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Relevance. The introduction of Varicella vaccine prophylaxis explains the need to develop a methodology for monitoring the vaccination effectiveness and the intensity of population immunity. This problem can be solved using quantitative immunoassay methods. Aim. Development of an enzyme-linked immunosorbent assay for the concentration of class G immunoglobulins (AB) to Varicella zoster virus (VZV) determining and assessing its functional characteristics and diagnostic efficiency. Materials and methods. Recombinant antigen GE VZV. WHO International Standard for Antibodies to VZV W1044. Blood serum samples from healthy people and patients with Chickenpox and Herpes zoster, blood serum samples containing IgG antibodies to herpes simplex viruses of the first and second types, cytomegalovirus, Epstein-Barr virus. Anti-VZV ELISA (IgG) reagent kit (Euroimmun, Germany). Indirect enzyme-linked immunosorbent assay. Immunization of animals with recombinant antigen GE, isolation, and purification of specific antibodies. Conjugation of monoclonal antibodies to human IgG with antibodies to antigen GE and with horseradish peroxidase. Results. An enzyme-linked immunosorbent assay in «an indirect» format has been developed to determine the specific antibodies to VZV concentration (IU/ml) in human serum/plasma. An artificial calibrator for determining the concentration of AB-VZV had been synthesized and standardized according to the International WHO-standard W1044. The main functional characteristics of the developed enzyme-linked immunosorbent assay are determined in accordance with GOST 51352-2013. The diagnostic kit was tested on blood serum samples from children with chickenpox (n = 43), adults with Herpes zoster (n = 158), healthy individuals (n = 781). The diagnostic sensitivity of the test system was 85%, the diagnostic specificity was 87% according to the ROC analysis. The absence of cross-reactivity of the test system was shown on samples with serological markers of other herpesvirus infections (n = 94). Comparative trials of the developed test system and its commercial analog, the Anti-VZV ELISA (IgG) reagent kit, did not reveal statistically significant differences between their functional characteristics. Conclusions. The developed test system for determining of the AB-VZV concentration in human serum/plasma in terms of its functional characteristics meets the GOST requirements, is characterized by high diagnostic efficiency, can be used to monitor the effectiveness of vaccine prophylaxis and strength of population immunity, as well as to assess the immune response in chickenpox and Herpes zoster.

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Measurement of Antibodies to Varicella-Zoster Virus Using a Virus-Free Fluorescent-Antibody-to-Membrane-Antigen (FAMA) Test

Cited by 8 publications

References 18 publications

Reevaluating immunization delays after red blood cell transfusion

Reevaluating immunization delays after red blood cell transfusion

Current Methods for the Detection of Antibodies of Varicella-Zoster Virus: A Review

Development of the ELISA Test System for Quantitative Determination of IgG to the Varizella zoster virus in Human Serum and Assessment of its Diagnostic Efficiency

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