Measurement of an analog of insulin-like growth factor-I in blood plasma using a novel enzyme-linked immunosorbent assay

Gajanandana, O.; Irvine, Kari R.; Grant, P. A.; Francis, G L; Knowles, S E; Wrin, Joe; Jc, Wallace; Owens, Philip

doi:10.1677/joe.0.1560407

Cited by 2 publications

(1 citation statement)

References 18 publications

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“…Manes et al [23] calculated a K D of 0.1 – 100 nM for IGF-1 and monoclonal antibodies on the basis of BIAcore™ SPR interaction analyses. Another group determined equilibrium constants of 0.01 – 0.15 nM for the interaction between monoclonal anti-IGF-1 antibodies and an IGF-1-analogue (LR3-IGF-1) by ELISA [24]. SPR analysis and also ELISA are methods based on interactions on solid surfaces.…”

Section: Resultsmentioning

confidence: 99%

Magneto-Optical Relaxation Measurements of Functionalized Nanoparticles as a Novel Biosensor

Aurich

Glöckl

Nagel

2009

Sensors

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Measurements of magneto-optical relaxation signals of magnetic nanoparticles functionalized with biomolecules are a novel biosensing tool. Upon transmission of a laser beam through a nanoparticle suspension in a pulsed magnetic field, the properties of the laser beam change. This can be detected by optical methods. Biomolecular binding events leading to aggregation of nanoparticles are ascertainable by calculating the relaxation time and from this, the hydrodynamic diameters of the involved particles from the optical signal. Interaction between insulin-like growth factor 1 (IGF-1) and its antibody was utilized for demonstration of the measurement setup applicability as an immunoassay. Furthermore, a formerly developed kinetic model was utilized in order to determine kinetic parameters of the interaction. Beside utilization of the method as an immunoassay it can be applied for the characterization of diverse magnetic nanoparticles regarding their size and size distribution.

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Section: Resultsmentioning

confidence: 99%