Measurement of activity of peroxidase isoenzymes in flax (<i>Linum usitatissimum</i>)

Hart, Michelle; Tyson, H.; Bloomberg, R.

doi:10.1139/b71-301

Cited by 32 publications

(15 citation statements)

References 3 publications

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“…SOD and APX activities were analysed by Native-PAGE (10% separating and 4% stacking gels). Specified staining of SOD was done according to the method of Beauchamp and Fridovich (1971), specified staining of APX was carried out following the procedure described by Rao et al (1996), POD (EC 1.11.1.7) isoforms were detected according to the method of Hart et al (1971), and finally to illustrate specified staining of CAT, we used Robertson et al (1987) method.…”

Section: Enzyme Activity Assay and Isoenzyme Analysismentioning

confidence: 99%

Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress

Ahmad¹,

Haddad²

2011

Czech J. Genet. Plant Breed.

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Abstract:The effect of silicon (Si) was investigated on the major antioxidant enzyme activities including superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX), peroxidase (POD), relative water content (RWC), chlorophyll and soluble protein contents, proline (Pro) and glycine betaine (GB) accumulation in three different growth stages (2 nd , 4 th leaf and tillering stages) of wheat (Triticum aestivum L.) plants under drought stress. The experiment was performed in a completely randomized design for three treatments including control, drought and Si-drought (2mM silicate sodium/kg) with three replications in a greenhouse. The results indicated that Si partially offset the negative impacts of drought stress increasing the tolerance of wheat by rising Pro and GB accumulation and soluble protein content. Compared with the plants treated with drought, applied Si significantly enhanced the activities of SOD, CAT, APX and POD. In contrast, drought stress caused a considerable decrease in RWC, chlorophyll and soluble protein contents. This Si effect was time-dependent and became stronger in the tillering stage. The results of the present experiment coincided with the conclusion that Si alleviates water deficit of wheat by preventing the oxidative membrane damage and may be associated with plant osmotic adjustment.

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Section: Enzyme Activity Assay and Isoenzyme Analysismentioning

confidence: 99%

Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress

Ahmad¹,

Haddad²

2011

Czech J. Genet. Plant Breed.

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“…GPX isoenzymes were analysed on 7.5% non-denaturing polyacrylamide gel at 4°C. The gels were loaded with protein equivalent of equal FW and were stained for GPX activity according to Hart et al (1971).…”

Section: Antioxidant Enzyme Isoformsmentioning

confidence: 99%

Antioxidative protection and proteolytic activity in tolerant and sensitive wheat (Triticum aestivum L.) varieties subjected to long-term field drought

Simova‐Stoilova

Demirevska

Petrova³

et al. 2009

Plant Growth Regul

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Field drought studies were performed in order to assess oxidative stress, proteolytic activity and yield loss under natural stress conditions. Flag leaves of two drought-tolerant (Yantar and Zlatitsa) and two drought-sensitive (Miziya and Dobrudjanka) winter wheat varieties were analyzed. Stress intensity was assessed by relative electrolyte leakage and proline accumulation. Senescence progression was followed by loss of chlorophyll and protein. Lipid peroxidation, H 2 O 2 content, activities of superoxide dismutase (SOD), catalase (CAT), and non-specific peroxidase (GPX) isoforms, as well as proteolytic activities were analyzed from heading throughout grain filling. Weakening of membrane integrity and oxidative damage to lipids were more pronounced in the sensitive varieties under field drought. The activities of Fe-and Cu/Zn SOD isoforms decreased in the controls, but remained high in drought-treated plants. The activities of

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“…CAT isoenzymes were stained according to Woodbury et al (1971). GPX isoenzymes were separated and revealed according to Hart et al (1971).…”

Section: Sds Page Of Soluble Leaf Proteinsmentioning

confidence: 99%

UV-B response of green and etiolated barley seedlings

Fedina¹,

Velitchkova²,

Georgieva³

et al. 2007

Biologia plant.

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7-d-old etiolated and green barley seedlings (Hordeum vulgare L. cv. Alfa) were irradiated with UV-B for 30 min and then kept for 24 h in light or darkness. Chlorophyll (Chl) synthesis was inhibited by about 30 % as a result of UV-B irradiation, but there were no significant changes in photochemical activity measured by variable to maximum fluorescence ratio (F v /F m ), quantum yield ( PS2 ) and oxygen evolution rate. Electron transport of etiolated seedlings was similar to that of green ones, nevertheless, the Chl content was more then 2-fold lower. Ribulose-1,5-bisphosphate carboxylase/oxygenase large and small subunits were diminished as a result of UV-B irradiation in etiolated and green plants, especially in those kept in the darkness. Catalase activity decreased and total superoxide dismutase activity increased in green and etiolated plants following UV-B treatment. When benzidine was used as a substrate, an isoform located between guaiacol peroxidases 2 and 3 (guaiacol peroxidase X) appeared, which was specific for UV-B treatment. As a result of irradiation, the contents of UV-B absorbing and UV-B induced compounds increased in green seedlings but not in etiolated seedlings.Additional key words: chlorophyll fluorescence, flavonoids, Hordeum vulgare, oxygen evolution, ribulose-1,5-bisphosphate carboxylase/oxygenase.

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Measurement of activity of peroxidase isoenzymes in flax (Linum usitatissimum)

Cited by 32 publications

References 3 publications

Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress

Study of silicon effects on antioxidant enzyme activities and osmotic adjustment of wheat under drought stress

Antioxidative protection and proteolytic activity in tolerant and sensitive wheat (Triticum aestivum L.) varieties subjected to long-term field drought

UV-B response of green and etiolated barley seedlings

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