Measurement of 8-oxo-2′-deoxyguanosine and 8-oxo-2′-deoxyadenosine in DNA and human urine by high performance liquid chromatography-electrospray tandem mass spectrometry

Analysis of 8-hydroxy-2′-deoxyguanosine in human urine using hydrophilic interaction chromatography with tandem mass spectrometry

“…fmol [17], 7 fmol (S/N=3) [18], 5 fmol (S/N=4) [19] and 7.5 fmol/injection (S/N=3) [27,31]. The low detection limit of method enables the measurement of basal levels of urinary 8-OHdG in non-exposed healthy subjects that were not quantified in the previous report [27,31].…”

“…The low detection limit of method enables the measurement of basal levels of urinary 8-OHdG in non-exposed healthy subjects that were not quantified in the previous report [27,31]. Furthermore, the analysis can be completed in 10 min and doesn't require washing and re-equilibrating the column, which makes it well suited for continuous analyses.…”

“…In addition, aqueous mobile phases that are used to retain polar compounds on reversed-phase columns are not suited for electrospray ionization (ESI) conditions [30]. Indeed, urinary 8-OHdG levels have even 5 been below the detection limit of LC-MS/MS method (7.5 fmol/injection, S/N= 3) [27,31]. Therefore, a more sensitive LC-MS/MS method is required to measure basal levels of urinary 8-OHdG in non-exposed healthy subjects.…”

Sucrose and IQ induced mutations in rat colon by independent mechanism

“…Liver cytosol was prepared as described elsewhere [17] and the products of protein oxidation, 2-amino adipic semialdehyde and ␥-glutamyl semialdehyde, were determined by HPLC [16]. The level of oxidative DNA damage in terms of 7-hydro-8-oxo-2 -deoxyguanosine (8-oxodG) was determined by HPLC-EC in liver and colon mucosa cells [18] and by HPLC MS/MS in urine [19]. In liver and colon mucosa cells DNA strand breaks were determined by the comet assay [11].…”

Development of Point-of-Care Testing Sensors for Biomarker Detection

“…The traditional methods for 8-OHdG quantitative analysis are high-performance liquid chromatography (HPLC) with electrochemical detection (ECD), 91,92 gas chromatographymass spectrometry (GC-MS), 93 HPLC tandem mass spectrometry, 94,95 and Enzyme-linked Immunosorbent Assay (ELISA). This POCT device would provide results in minutes rather than days or weeks and eliminate the concerns involved with the transport and storage of biological samples.…”