Measurement of 4-hydroxyphenylacetic aciduria as a screening test for small-bowel disease.

Chalmers, R. J.; Valman, H B; Liberman, Meytal

doi:10.1093/clinchem/25.10.1791

Cited by 37 publications

(21 citation statements)

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“…In our study, 4-hydroxybenzeneacetic acid was higher in the CHD group than in the control group ( OR =4.74,95% CI: 1.06-21.06). As reported, tyrosine is converted from phenylalanine under the action of intestinal bacteria [20], and tyrosine decarboxylation is further converted into 4-hydroxybenzeneacetic acid [21]. It can be seen that the increase in 4-hydroxybenzeneacetic acid in the case group may be due to a disorder of microecological bacteria in the pregnant mother of the CHD fetus or the increasing of tyrosine.…”

Section: Discussionmentioning

confidence: 90%

Study on the Potential Biomarkers of Maternal Urine Metabolomics for Fetus with Congenital Heart Diseases Based on Modified Gas Chromatograph-Mass Spectrometer

Xie

Luo

Xiong

et al. 2019

BioMed Research International

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Background. There has been significant research on the genetic and environmental factors of congenital heart defects (CHDs), but few causes of teratogenicity, especially teratogenic mechanisms, can be clearly identified. Metabolomics has a potential advantage in researching the relationship between external factors and CHD. Objective. To find and identify the urinary potential biomarkers of pregnancy (including in the second and third trimesters) for fetuses with CHD based on modified gas chromatograph-mass spectrometer (GC-MS), which could reveal the possibility of high-risk factors for CHD and lay the foundation for early intervention, treatment, and prevention. Methods. Using a case-control design, we measured the urinary potential biomarkers of maternal urine metabolomics based on GC-MS in a population-based sample of women whose infants were diagnosed with CHD (70 case subjects) or were healthy (70 control subjects). SIMCA-P 13.0 software, principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), Wilcoxon-Mann-Whitney test, and logistics regression were used to find significant potential biomarkers. Result. The 3D score graph of the OPLS-DA showed that the CHD and control groups were fully separated. The fitting parameters were R2x=0.78 and R2y=0.69, and the forecast rate was Q2=0.61, indicating a high forecast ability. According to the ranking of VIPs from the OPLS-DA models, we found 34 potential metabolic markers with a VIP > 1, and after two pairwise rank sum tests, we found 20 significant potential biomarkers, which were further used in multifactor logistic regressions. Significant substances, including 4-hydroxybenzeneacetic acid (OR=4.74, 95% CI: 1.06-21.06), 5-trimethylsilyloxy-n-valeric acid (OR=15.78, 95% CI: 2.33-106.67), propanedioic acid (OR=5.37, 95% CI: 1.87-15.45), hydracrylic acid (OR=6.23, 95% CI: 1.07-36.21), and uric acid (OR=5.23, 95% CI: 1.23-22.32), were associated with CHD. Conclusion. The major potential biomarkers in maternal urine associated with CHD were 4-hydroxybenzeneacetic acid, 5-trimethylsilyloxy-n-valeric acid, propanedioic acid, hydracrylic acid, and uric acid, respectively. These results indicated that the short chain fatty acids (SCFAs) and aromatic amino acid metabolism may be relevant with CHD.

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Section: Discussionmentioning

confidence: 90%

Study on the Potential Biomarkers of Maternal Urine Metabolomics for Fetus with Congenital Heart Diseases Based on Modified Gas Chromatograph-Mass Spectrometer

Xie

Luo

Xiong

et al. 2019

BioMed Research International

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“…Small intestinal bacterial overgrowth is frequent in CF . One of the bacterial metabolites with increased concentration, hydroxyphenylacetic acid, has been described previously to be increased in urine of CF patients and is also a marker of small bowel bacterial overgrowth syndrome in non‐CF patients . This dysbiosis seen in CF is likely secondary to a combination of altered motility, bacterial overgrowth, use of antibiotics and abnormal enterohepatic metabolism of bile acids.…”

Section: Discussionmentioning

confidence: 99%

Serum metabolomics indicate altered cellular energy metabolism in children with cystic fibrosis

et al. 2013

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“…Another feasible application of metabolic profiling is the evaluation of intestinal disease. Thus, 4-hydroxyphenylacetic acid, an intestinal bacterial flora metabolite, was found to be a marker of small-bowel disease due to bacterial overgrowth as and in the case of Giardia infestation ( 29 ). The utilization of OAs as disease markers is still limited due to the lack of stringent, reproducible RVs of healthy controls, as well as to difficulties when comparing data from different platforms ( 30 ).…”

Section: Discussionmentioning

confidence: 99%

Application of metabolomics: Focus on the quantification of organic acids in healthy adults

Tsoukalas

Alegakis

Fragkiadaki

et al. 2017

International Journal of Molecular Medicine

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Metabolomics, a 'budding' discipline, may accurately reflect a specific phenotype which is sensitive to genetic and epigenetic interactions. This rapidly evolving field in science has been proposed as a tool for the evaluation of the effects of epigenetic factors, such as nutrition, environment, drug and lifestyle on phenotype. Urine, being sterile, is easy to obtain and as it contains metabolized or non-metabolized products, is a favored study material in the field of metabolomics. Urine organic acids (OAs) reflect the activity of main metabolic pathways and have been used to assess health status, nutritional status, vitamin deficiencies and response to xenobiotics. To date, a limited number of studies have been performed which actually define reference OA values in a healthy population and as reference range for epigenetic influences, and not as a reference to congenital metabolic diseases. The aim of the present study was thus the determination of reference values (RVs) for urine OA in a healthy adult population. Targeted metabolomics analysis of 22 OAs in the urine of 122 healthy adults by gas chromatography-mass spectrometry, was conducted. Percentile distributions of the OA concentrations in urine, as a base for determining the RVs in the respective population sample, were used. No significant differences were detected between female and male individuals. These findings can facilitate the more sensitive determination of OAs in pathological conditions. Therefore, the findings of this study may contribute or add to the information already available on urine metabolite databases, and may thus promote the use of targeted metabolomics for the evaluation of OAs in a clinical setting and for pathophysiological evaluation. However, further studies with well-defined patients groups exhibiting specific symptoms or diseases are warranted in order to discern between normal and pathological values.

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Measurement of 4-hydroxyphenylacetic aciduria as a screening test for small-bowel disease.

Cited by 37 publications

References 0 publications

Study on the Potential Biomarkers of Maternal Urine Metabolomics for Fetus with Congenital Heart Diseases Based on Modified Gas Chromatograph-Mass Spectrometer

Study on the Potential Biomarkers of Maternal Urine Metabolomics for Fetus with Congenital Heart Diseases Based on Modified Gas Chromatograph-Mass Spectrometer

Serum metabolomics indicate altered cellular energy metabolism in children with cystic fibrosis

Application of metabolomics: Focus on the quantification of organic acids in healthy adults

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