Measurement of 3-Hydroxyanthranilic Acid and Anthranilic Acid in Urine by High-performance Liquid Chromatography

Urinary Excretory Ratio of Anthranilic Acid/Kynurenic Acid as an Index of the Tolerable Amount of Tryptophan

“…The urinary content of Nam, 2-Py and 4-Py was simultaneously measured by the HPLC method of Shibata et al, 8) and that for MNA by the method of Shibata. 9) Urinary concentration of 3-HA was measured by the HPLC method of Shibata and Onodera, 10) while the urinary concentration of KA was measured by the method of Shibata. 11) The urinary concentration of XA was measured by the method of Shibata and Onodera, 12) and QA was measured by the method of Mawatari et al 13) Statistical analysis.…”

Pharmacological Doses of Nicotinic Acid and Nicotinamide Are Independently Metabolized in Rats

“…Nam, 2-Py, and 4-Py were simultaneously measured by HPLC and so were XA and 3-HA (17, 24). MNA, KA, Nam N-oxide, and AnA were also measured by HPLC (16,19,25,26). QA and 3-HK were converted to fluorometric compounds and then measured by HPLC (27,28).…”

Difference in Tryptophan-nicotinamide Conversion According to Dietary Nitrogen Sources; Casein, Casein Hydrolysate, and Mixtures of Amino Acids

“…These homogenates were used as enzyme sources for measuring the activitie~ of Trp' oxygenase, kynureninase, kynurenine aminotransferase, Nam methyl transferase, ,2-Py-formirig MNA oxidase, and 4~Py-forming MNA oxidase. One part of each homogenate w&s centrifuged at 15,000 x 9 for 20 min to measure the activities, of 3-hydroxyanthranilic acid oxygenase and aminocarboxymuconate:semialdehyde decarboxylase. The total Nam in the 'liver was extracted as described in the literature.…”