Measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method

Chen, Huiping; McCoy, Leslie F.; Schleicher, Rosemary L.; Pfeiffer, Christine M.

doi:10.1016/j.cca.2008.01.017

Cited by 116 publications

(84 citation statements)

References 19 publications

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“…Studies on the levels of 25(OH)D2 in adults are limited and the results are mixed; however, the majority have demonstrated higher concentrations than that found in our study. [16][17][18] It should be mentioned that the low proportion of individuals in the cohort with 25(OH) D2 levels >0.5 ng/mL (12%) did not enable evaluation of the correlation between vitamin D2 and the studied variables.…”

Section: Discussionmentioning

confidence: 99%

Smoking is a significant determinant of low serum vitamin D in young and middle-aged healthy males

Kassi¹,

Stavropoulos²,

Kokkoris³

et al. 2014

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Section: Discussionmentioning

confidence: 99%

Smoking is a significant determinant of low serum vitamin D in young and middle-aged healthy males

Kassi¹,

Stavropoulos²,

Kokkoris³

et al. 2014

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“…Recently, liquid chromatography tandem mass spectrometry technique (LC-MSMS) has been utilized and this is currently considered to be the most accurate technique for these measurements [19,20] . Other strengths of our study are the large number of cases examined and adjustment of the measured concentrations for the maternal factors that affect this measurement.…”

Section: Discussionmentioning

confidence: 99%

Normal Range of Maternal Serum Vitamin D at 11–13 Weeks’ Gestation

Ertl

Brawura-Biskupski-Samaha

et al. 2011

Fetal Diagn Ther

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Objective: To establish a normal range of maternal serum levels of total vitamin D at 11–13 weeks’ gestation and examine factors from maternal characteristics and obstetric history affecting these levels. Methods: Serum 25-hydroxyvitamin D [25(OH)D] was measured by liquid chromatography-tandem mass spectrometry in 1,000 singleton pregnancies with normal outcome. Multiple regression analysis was used to determine the factors from maternal characteristics and obstetric history affecting the measured 25(OH)D levels and the regression model was used to convert each measurement into a multiple of the median (MoM). Results: Serum 25(OH)D increased with maternal age, decreased with body mass index (BMI), it was higher in the summer than other months and when conception was assisted than spontaneous and it was lower in cigarette smokers and in women of African and Asian racial origin compared to Caucasians. In Caucasian, non-smoking women, with BMI below 25, conceiving spontaneously and sampled in the summer months the estimated 5th, 10th, 50th, 90th and 95th percentiles of 25(OH)D were 10.8, 14.7, 30.7, 48.2 and 55.7 ng/ml, respectively. Conclusion: Maternal serum 25(OH)D concentration at 11–13 weeks is affected by season of blood sampling and maternal characteristics.

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“…Some of them even offer a sensitivity of 3-4 nmol/l, which should be sufficient to detect vitamin D deficiency. The current automated vitamin D assays appear in general to show acceptable correlation with reference LC-MS/MS methods (68,69,70,71). Nevertheless, LC-MS/MS is considered by experts the best available technique to measure 25-OHD and there are strong recommendations to replace IAs whenever possible with the former (72).…”

Section: Oestradiolmentioning

confidence: 99%

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

Taylor¹,

Keevil²,

Huhtaniemi³

2015

European Journal of Endocrinology

252

157

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SummaryThe recent onslaught of mass spectrometry (MS) to measurements of steroid hormones, including demands that they should be the only acceptable method, has confused clinicians and scientists who have relied for more than 40 years on a variety of immunoassay (IA) methods in steroid hormone measurements. There is little doubt that MS methods with their superior specificity will be the future method of choice in many clinical and research applications of steroid hormone measurement. However, the majority of steroid measurements are currently, and will continue to be, carried out using various types of IAs for several reasons, including their technical ease, cost and availability of commercial reagents. Speedy replacement of all IAs with MS is an unrealistic and unnecessary goal, because the availability of MS measurements is limited by cost, need of expensive equipment, technical demands and lack of commercial applications. Furthermore, IAs have multiple well-known advantages that vindicate their continuing use. The purpose of this article is to elucidate the advantages and limitations of the MS and IA techniques from two angles, i.e. promotion of MS and defence of IA. The purpose of the text is to give the reader an unbiased view about the current state and future trends of steroid analysis and to help him/her choose the correct assay method to serve his/her diagnostic and research needs.

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Measurement of 25-hydroxyvitamin D3 (25OHD3) and 25-hydroxyvitamin D2 (25OHD2) in human serum using liquid chromatography-tandem mass spectrometry and its comparison to a radioimmunoassay method

Cited by 116 publications

References 19 publications

Smoking is a significant determinant of low serum vitamin D in young and middle-aged healthy males

Smoking is a significant determinant of low serum vitamin D in young and middle-aged healthy males

Normal Range of Maternal Serum Vitamin D at 11–13 Weeks’ Gestation

Mass spectrometry and immunoassay: how to measure steroid hormones today and tomorrow

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