Measurement and significance of sperm morphology

Menkveld, Roelof; Holleboom, Cas A G; Rhemrev, Johann

doi:10.1038/aja.2010.67

Cited by 146 publications

(119 citation statements)

References 69 publications

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“…24 Sperm morphology assessment, when performed according to welldefined procedures, gives data of high predictive value for male fertility potential. 29 However, in our study, 7% of the NCR respondents did not analyse this parameter at all. For years, sperm morphology has been recognized as probably the most confusing and subjective area of semen analysis.…”

Section: Variations In Methods Of Semen Analysis In Poland R Walczak-mentioning

confidence: 42%

Semen analysis standardization: is there any problem in Polish laboratories?

Walczak-Jędrzejowska¹,

Marchlewska²,

Oszukowska³

et al. 2013

Asian J Androl

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The aim of the study was to determine the degree of compliance of Polish laboratories with World Health Organization (WHO) recommendations, with regard to semen analysis methodology. A survey requesting information about methods of semen analysis was distributed to employees of 55 laboratories. Respondents who had participated in external seminological workshops (31%) were termed certified respondents (CR), the remaining (69%)-non-certified respondents (NCR). Only one laboratory (6%) in the CR group and none in the NCR were compliant with WHO guidelines for methods and equipment used to evaluate seminal volume, sperm motility, concentration, vitality and morphology. Most problems were of volume measurement (weighing method was reported by 17% of CR and 10% of NCR) and staining method for sperm morphology (Papanicolau or Diff-Quik were found in 33% of CR and 23% of NCR). A three-or four-point grading of sperm motility was used by the majority of respondents; however, 17% of CR and 37% of NCR did not use a laboratory counter to tally spermatozoa. Although a haemocytometer method was used by 80% of laboratories in each group, the improved Neubauer chamber was used only by 42% of CR and 19% of NCR. In each group, 24% of laboratories did not perform a vitality test. Procedural errors and the interchangeable utilization of two or even three methods to analyse a given parameter was observed in both groups. The results indicate a need for standardisation of the methods and continuous, unified training in semen analysis in Polish laboratories.

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Section: Variations In Methods Of Semen Analysis In Poland R Walczak-mentioning

confidence: 42%

Semen analysis standardization: is there any problem in Polish laboratories?

Walczak-Jędrzejowska¹,

Marchlewska²,

Oszukowska³

et al. 2013

Asian J Androl

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show abstract

“…In conclusion, if sperm morphology is done correctly and with care, and with strict application of the guidelines as outlined in the 2010 WHO manual, sperm morphology measurement still has a very important role to play in the clinical evaluation of male fertility potential. 95 …”

Section: Discussionmentioning

confidence: 99%

Semen analysis and sperm function testing

Franken¹,

Oehninger²

2011

Asian J Androl

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Despite controversy regarding the clinical value of semen analysis, male fertility investigation still relies on a standardized analysis of the semen parameters. This is especially true for infertility clinics in both developing and developed countries. Other optional tests or sophisticated technologies have not been widely applied. The current review addresses important changes in the analysis of semen as described in the new World Health Organization (WHO) manual for semen analysis. The most important change in the manual is the use of evidence-based publications as references to determine cutoff values for normality. Apart from the above mentioned changes, the initial evaluation and handling methods remain, in most instances, the same as in previous editions. Furthermore, the review evaluates the importance of quality control in andrology with emphasis on the evaluation of sperm morphology. WHO sperm morphology training programmes for Sub-Saharan countries were initiated at Tygerberg Hospital in 1995. The external quality control programme has ensured that the majority of participants have maintained their morphological reading skills acquired during initial training. This review reports on current sperm functional tests, such as the induced acrosome reaction, and sperm-zona pellucida binding assays, as well as the impact of sperm quality in terms of DNA integrity, and the relationship of sperm function tests to sperm morphology.

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“…Это приводит к низким показателям оплодотворения при проведе-нии классического ЭКО и/или ЭКО с применением микрохирургической техники оплодотворения ме-тодом интрацитоплазматической инъекции еди-ничного сперматозоида в цитоплазму ооцита (ИКСИ). Вероятными причинами образования га-мет с данной патологией являются гипертермия яичек и оксидативный стресс: большая доля спер-матозоидов с удлиненными головками часто выяв-ляется у мужчин с инфекциями урогенитального тракта и при варикоцеле [17]. Утолщение прослой-ки жировой ткани в мошонке, наблюдающееся у мужчин с избыточной массой тела и ожирением, также приводит к гипертермии яичек, увеличению количества свободных радикалов и оксидативному стрессу, что, по мнению K. Shiraishi и соавт.…”

Section: патология головкиunclassified

“…Влияние ИМТ на патологию головки сперматозоидов По данным литературы [15,17,23,24], одними из наиболее тяжелых патологий структуры сперматозо-идов являются элонгация головки и микроцефалия. Согласно исследованиям, у гамет с удлиненными и микроголовками отмечаются повреждения акросо-мы и нарушения в шейке, высокий индекс фрагмен-тации ДНК.…”

Section: влияние имт на подвижность сперматозоидовunclassified