In this paper we study continuous semigroups of positive operators on general vector lattices equipped with the relative uniform topology τ ru . We introduce the notions of strong continuity with respect to τ ru and relative uniform continuity for semigroups. These notions allow us to study semigroups on non-locally convex spaces such as L p (R) for 0 < p < 1 and non-complete spaces such as Lip(R), UC(R), and C c (R). We show that the (left) translation semigroup on the real line, the heat semigroup and some Koopman semigroups are relatively uniformly continuous on a variety of spaces.