Measles virus-specific immunoglobulin G subclass response in serum and cerebrospinal fluid

Narita, Mitsuo; Yamada, Satoshi; Matsuzono, Yoshihiro; Itakura, Osamu; Togashi, Takehiro; Kikuta, Hídeaki

doi:10.1016/s0928-0197(97)10007-1

Cited by 13 publications

(9 citation statements)

References 19 publications

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“…Interestingly although our patient's IgG was high when she first presented in April 1999, titres rose even higher during the next 2 weeks. The detection of IgG3 as well as IgG1 in Nipah virus infection is similar to the IgG subclass responses seen in measles and subacute sclerosing panencephalitis 15. Unfortunately no specimens were taken during her original febrile illness in December 1998, but by comparison with other Nipah patients we expect her IgM would have been positive then.…”

Section: Discussionsupporting

confidence: 57%

Late presentation of Nipah virus encephalitis and kinetics of the humoral immune response

Wong

2001

Journal of Neurology, Neurosurgery &Amp; Psychiatry

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Nipah virus is a newly discovered paramyxovirus transmitted directly from pigs to humans. During a large encephalitis outbreak in Malaysia and Singapore in 1998-9, most patients presented acutely. A 12 year old child is described who developed encephalitis 4 months after exposure to the virus. She was diagnosed by a new indirect IgG enzyme linked immunosorbent assay (ELISA), which is also described. The late presentation and IgG subclass responses had similarities to subacute sclerosing panencephalitis. Nipah virus should be considered in patients with encephalitis even months after their possible exposure. (J Neurol Neurosurg Psychiatry 2001;71:552-554)

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Section: Discussionsupporting

confidence: 57%

Late presentation of Nipah virus encephalitis and kinetics of the humoral immune response

Wong

2001

Journal of Neurology, Neurosurgery &Amp; Psychiatry

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“…In spite of that, IgG3 was not used, until now, as an IgM-like antibody response, that is, as a serological index of recent measles infection. This could be attributed to a lower rate of IgG3 detection as compared with that reported for IgM antibody in the early immune response (8,14).…”

Section: Discussionmentioning

confidence: 73%

“…Narita et al suggested that the IgG3 response could play a major role in acute-phase immunity during primary infection, while the IgG1 response could be related to maintenance of measles immunity (14).…”

mentioning

confidence: 99%

Measles Virus-Specific Immunoglobulin G Isotype Immune Response in Early and Late Infections

et al. 2001

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A total of 154 human serum samples (32 acute-phase and 22 convalescent-phase serum samples obtained within a week and between days 8 and 26 after the onset of rash, respectively, and 100 samples drawn from healthy immune adults) were processed by an immunofluorescence assay for the detection of immunoglobulin M (IgM), total immunoglobulin G (IgG), IgG1, IgG2, IgG3, and IgG4 measles virus-specific antibodies. In the acute phase, IgG1 was seen first, followed by IgG2, IgG3, and IgG4 responses, the mean seropositivity of which gradually increased during convalescence, reaching 100% (standard deviation [SD], 84 to 100%), 57% (SD, 34 to 80%), 86% (SD, 66 to 100%), and 86% (SD, 66 to 100%), respectively. IgG2 rose and fell in connection with Measles has been targeted for global eradication by the World Health Organization's Expanded Programme on Immunization; for the effective control and eventual eradication of measles, it is necessary to impair measles transmission by establishing herd immunity. To accomplish this aim, a sensitive surveillance system is essential to detect wild-virus circulation, as well as sensitive and specific diagnostic tests (4, 5). The diagnosis of measles infection is serologically confirmed by the presence of a fourfold rise in antibody titers for paired acuteand convalescent-phase sera or most often by detection of anti-measles virus immunoglobulin M (IgM) antibody. The performance of IgM detection for the differentiation of primary and secondary measles antibody responses depends upon (i) propagation of the virus within the community, (ii) characteristics of the individual immune response, (iii) time of specimen collection, and (iv) assay sensitivity.Similar to other serological markers, a subclass-restricted response to antigens has been recently demonstrated (8, 9, 10, 12, 18); however, a limited amount of data is available on the virus-specific immunoglobulin G (IgG) subclass responses during the ordinary course of measles viral infection. Narita et al. suggested that the IgG3 response could play a major role in acute-phase immunity during primary infection, while the IgG1 response could be related to maintenance of measles immunity (14).These data offer early support for the hypothesis that the IgG isotypic immune response could also be a useful serologic tool, in addition to specific measles IgM antibody detection, to eventually distinguish between early and late measles infection.The present study was undertaken to point out the specific antiviral IgG1, IgG2, IgG3, and IgG4 subclass response patterns elicited during natural infection (acute and convalescent phases) as well as in the long-lasting humoral immunity to measles virus.The aim of this paper is to contribute to the global understanding of antibody responses to measles virus infections. MATERIALS AND METHODSSerum specimens. A total of 154 positive human serum samples for measles antibodies were used in this study. Serum specimens were classified within two groups according to the characteristics of the measles cases from whic...

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“…No role has been described for IgA in protection against blood-stage merozoites [38], however the induction of this isotype may be more relevant to the clinical development of the adenovirus-MVA vaccine platform against mucosal pathogens including HIV-1 [15], [17]. The strong induction of the cytophilic human IgG1 and IgG3 isotypes is consistent with a viral-based vaccine delivery platform [61], [62], and would also be of relevance to other pathogen targets, including HIV-1, where antibody-mediated cellular effector functions or specific IgG isotypes may be associated with protection [63]. In the context of blood-stage malaria immunity, the interaction of IgG1 and IgG3 with monocytes has been reported to be important for mediating effective antibody-dependent cellular inhibition (ADCI) via monocytes when using antibodies present in the serum of naturally exposed individuals [64]–[66] or those specific for antigens such as MSP3 or GLURP [67]–[69].…”

Section: Discussionmentioning

confidence: 80%

Assessment of Humoral Immune Responses to Blood-Stage Malaria Antigens following ChAd63-MVA Immunization, Controlled Human Malaria Infection and Natural Exposure

et al. 2014

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The development of protective vaccines against many difficult infectious pathogens will necessitate the induction of effective antibody responses. Here we assess humoral immune responses against two antigens from the blood-stage merozoite of the Plasmodium falciparum human malaria parasite – MSP1 and AMA1. These antigens were delivered to healthy malaria-naïve adult volunteers in Phase Ia clinical trials using recombinant replication-deficient viral vectors – ChAd63 to prime the immune response and MVA to boost. In subsequent Phase IIa clinical trials, immunized volunteers underwent controlled human malaria infection (CHMI) with P. falciparum to assess vaccine efficacy, whereby all but one volunteer developed low-density blood-stage parasitemia. Here we assess serum antibody responses against both the MSP1 and AMA1 antigens following i) ChAd63-MVA immunization, ii) immunization and CHMI, and iii) primary malaria exposure in the context of CHMI in unimmunized control volunteers. Responses were also assessed in a cohort of naturally-immune Kenyan adults to provide comparison with those induced by a lifetime of natural malaria exposure. Serum antibody responses against MSP1 and AMA1 were characterized in terms of i) total IgG responses before and after CHMI, ii) responses to allelic variants of MSP1 and AMA1, iii) functional growth inhibitory activity (GIA), iv) IgG avidity, and v) isotype responses (IgG1-4, IgA and IgM). These data provide the first in-depth assessment of the quality of adenovirus-MVA vaccine-induced antibody responses in humans, along with assessment of how these responses are modulated by subsequent low-density parasite exposure. Notable differences were observed in qualitative aspects of the human antibody responses against these malaria antigens depending on the means of their induction and/or exposure of the host to the malaria parasite. Given the continued clinical development of viral vectored vaccines for malaria and a range of other diseases targets, these data should help to guide further immuno-monitoring studies of vaccine-induced human antibody responses.

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Measles virus-specific immunoglobulin G subclass response in serum and cerebrospinal fluid

Cited by 13 publications

References 19 publications

Late presentation of Nipah virus encephalitis and kinetics of the humoral immune response

Late presentation of Nipah virus encephalitis and kinetics of the humoral immune response

Measles Virus-Specific Immunoglobulin G Isotype Immune Response in Early and Late Infections

Assessment of Humoral Immune Responses to Blood-Stage Malaria Antigens following ChAd63-MVA Immunization, Controlled Human Malaria Infection and Natural Exposure

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