Measles virus protein‐specific IgM, IgA, and IgG subclass responses during the acute and convalescent phase of infection

Abstract

The availability of new generation serological assays allowed re-evaluation of the antibody response to measles virus. IgM, IgA, total IgG, and IgG subclass responses were studied to the three major immunogenic measles virus proteins: the fusion protein (F), haemagglutinin (H), and nucleoprotein (N). Plasma samples were obtained from clinically diagnosed measles cases (n = 146) in Khartoum (Sudan) within a week after onset of the rash. Convalescent phase samples were collected from 32 of 117 laboratory-confirmed measles cases at different time points after onset of rash. Glycoprotein-specific IgM, IgG, and IgA antibody levels correlated well to the N-specific response. For IgG and IgA, responses to F were higher than to H. IgA antibody levels were undetectable in about one third of the laboratory-confirmed cases during the acute phase, but positive in all patients tested 1-4 weeks after infection. IgM levels declined rapidly and were lost 3-6 months after infection. IgA levels declined slowly during the first year but did not return to background levels during the subsequent 2 years. IgG avidity maturation was detected during a 3-6 month period after infection. The predominant IgG subclasses during the acute phase were IgG(1) and IgG(3). The latter was lost in the convalescent phase, while the IgG(4) isotype showed a slight rise afterwards. Interestingly, acute phase IgG(3) and IgA responses were associated, and were only detected in samples with high IgG. This study provides a comprehensive perspective on the antibody response to wild-type measles virus infection.

Age-Dependent Features of Evolving Humoral Immunity in Children

“…Почему же тогда у маленьких детей, несмотря на большое количество В1-лимфоцитов, в кро-ви определяются буквально следы IgA? По-видимому, это связано с тем, что плазматические клетки, производные от В1-лимфоцитов, распо-ложены в основном в слизистых и синтезируют львиную долю секреторных IgA [12] [8,11], IgG2-антитела стимулируются углеводными антигена-ми [17], тогда как IgG4, наиболее вероятно, игра-ют важную роль при хронической антигенной стимуляции [18,19]. Однако все не так просто.…”

A Study on the Clinical Application of a Rapid Diagnostic Reagent for Measles

“…Also rise of serum IgM antibody is a biological reaction due to infections (25)(26)(27), the sensitivity and specificity of difference of immunochromatography and had been estimated in this study.…”

“…Commercially available EIA kits were used to detect serum antibodies against measles (25)(26)(27). Using serum and extract liquid residual by using real-time RT-PCR and antibody measurement conducted.…”

Measles-specific Neutralizing Antibodies in Rural Mozambique: Seroprevalence and Presence in Breast Milk

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