Meaningful connections: Interrogating the role of physical fibroblast cell–cell communication in cancer

Gardiner, Jaye C.; Cukierman, Edna

doi:10.1016/bs.acr.2022.01.004

Cited by 10 publications

(11 citation statements)

References 152 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[27][28][29] Previous studies found that fibroblasts participate in important intercellular interactions in kidney injury, skin wound repair, and cancer. [30][31][32] These results suggest that fibroblasts in rat ileal tissue may affect intestinal damage and repair processes through intercellular interactions during SAP-induced intestinal injury and MAGL therapy.…”

Section: Discussionmentioning

confidence: 77%

Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis

Wang,

Xu,

et al. 2023

JIR

View full text Add to dashboard Cite

To explore the molecular mechanisms of intestinal injury and treatment by analyzing changes in cellular heterogeneity and composition in rat ileal tissue during injury and treatment processes. Methods:We constructed a rat model of SAP and evaluated treatment with an injected of monoacylglycerol lipase (MAGL) inhibitor (JZL184) solution using three experimental groups: healthy male Sprague-Dawley (SD) rats injected with vehicle (CON), male SD SAP model rats injected with vehicle (SAP), and male SAP rats injected with JZL184. We obtained and prepared a single-cell suspension of ileal tissue of each rat for single-cell transcriptome sequencing. Results: This project classified changes in cellular heterogeneity and composition in rat ileal tissue during SAP-induced intestinal injury and MAGL treatment. We found that the number of fibroblast clusters was decreased in the SAP group relative to the CON group, and increased after JZL184 treatment. Further analysis of differences in gene expression between cell clusters in each group reveals that fibroblasts had the greatest number of differentially expressed genes. Most notably, expression of genes involved in communication between cells was found to vary during SAP-induced intestinal injury and JZL184 treatment. Among these changes, the degree of difference in expression of genes involved in communication between fibroblasts and other cells was the highest, indicating that fibroblasts in rat ileal tissue affect intestinal injury and repair through cell-to-cell communication. In addition, our results reveal that differentially expressed RNA-binding proteins in fibroblasts may affect their functions in intestinal injury and treatment by affecting the expression of genes regulating communication between cells. Conclusion: These findings emphasize the importance of understanding the interactions between fibroblasts and other cells in the context of intestinal injury, providing valuable insights for further exploring molecular mechanisms and insight for discovering new treatment targets and strategies.

show abstract

Section: Discussionmentioning

confidence: 77%

Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis

Wang,

Xu,

et al. 2023

JIR

View full text Add to dashboard Cite

show abstract

“…Recent studies have confirmed the existence of TNTs in various types of cells, especially in different kinds of immune cells [77]. Direct contact of CAFs with cancer cells has been proposed [36,78,79].…”

Section: Communication Between Cells Describes the Way A Cell Can Pro...mentioning

confidence: 94%

“…The types of contacts that these cells make with the ECM are among the most unique and have critical implications for TME biology. For example, CAF-ECM adhesions have been shown to have significant effects on CAF-regulated functions and represent stromal signatures predictive of overall patient survival [26,36].…”

Section: Extracellular Matrixmentioning

confidence: 99%

“…Extracellular pathways are used first, for example, in the secretion of signaling molecules (e.g., exosomes, cytokines, and metabolites). Physical communication describes direct interactions between cells that change the behavior of neighboring cells [36]. Intercellular contacts are crucial for the existence of the organism, in particular, for the emergence and evolution of tumors.…”

Section: Communication Between Cells Describes the Way A Cell Can Pro...mentioning

confidence: 99%

See 1 more Smart Citation

The Untouched Reserve of Cancer Chemotherapy: Tumor Cell Communication as a Promising Supramolecular Target

Alekseenko,

Zhukova,

Kondratyeva

et al. 2024

Preprint

View full text Add to dashboard Cite

52 years have passed since President Nixon launched the "War on Cancer". The goals outlined by the President were not achieved because cancer treatment applied such as chemotherapy, radiotherapy, and targeted therapy have not fully met expectations. We suggest a new chemotherapeutic strategy: disrupting the communication between cancer cells and their microenvironment by chemical means. Immunological synapses that form between cancer cells and immune or other stromal cells provide an attractive target for this approach. Synapses form ligand-receptor clusters within interface of the interacting cells. Despite their differences, synapses share common properties: intercellular protein clusters; the proximity of these proteins; and their cooperative interaction making a synapse an unified functional unit. Synapses provide the limited space for the focused intercellular exchange of signaling molecules and particles. Therefore, destruction of synapses is expected to cause collapse of various tumor types. Additionally, the clustered arrangement of synapse components offers opportunities to increase treatment safety by reducing the concentration of cell impermeable agents used, to enhance its specificity applying cross-linking reagents, thus restricting modifications of surface-exposed molecules. By attaching a cleavable cell permeable toxic agent to a crosslinker should further enhance a killing potential in treating cancer. The proposed approach promises to be simple, universal, and less expensive than existing cancer therapy methods.

show abstract

“…LATS2 was lowexpressed in ESCC, which may explain the shorter survival rate. In addition, LATS2 may be involved in cell migration (Yamaguchi and Condeelis, 2007;Duff and Long, 2017), adhesion (Läubli and Borsig, 2019), and connection (Wu et al, 2017;Gardiner and Cukierman, 2022), which are closely related to tumor occurrence and development. Additionally, we assessed the association between LATS2 expression and key molecules associated with the immune system.…”

Section: Introductionmentioning

confidence: 99%

Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

et al. 2022

View full text Add to dashboard Cite

According to the TIMER database, large tumor suppressor 2 (LATS2) is differentially expressed in various tumors. However, the correlation between LATS2 and esophageal squamous cell carcinoma (ESCC) and the association between LATS2 and immune infiltration in ESCC remain unclear. Our synthetic research on LATS2 in ESCC revealed that the expression was low in esophageal squamous epithelium tissues, revealing the pernicious and adverse prognosis of ESCC. The Kaplan–Meier survival investigation pointed out that low LATS2 expression would result in an adverse prognosis. Biological investigation indicated that LATS2 was engaged in cell migration, adhesion, and junction. To further explore the relationship between LATS2 and tumor immunity, we utilized CIBERSORT to assess immune infiltration. The findings revealed that specimens with lower LATS2 expression showed higher immune infiltration, including T-cell follicular helper cells, M0 macrophages, M1 macrophages, and myeloid dendritic cell resting. An association investigation indicated that LATS2 was negatively relevant to immune checkpoints that restrain operative antitumor immune reactions. We also conducted immunohistochemical staining to explore the link between LATS2 expression and immunophenotype. The indicated association between low LATS2 expression and an immunophenotype is conducive to our understanding of ESCC mini-environments and might offer new indications for enhancing new therapeutic targets.

show abstract

Meaningful connections: Interrogating the role of physical fibroblast cell–cell communication in cancer

Cited by 10 publications

References 152 publications

Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis

Single-Cell RNA Sequencing Reveals Roles of Fibroblasts During Intestinal Injury and Repair in Rats with Severe Acute Pancreatitis

The Untouched Reserve of Cancer Chemotherapy: Tumor Cell Communication as a Promising Supramolecular Target

Identifying LATS2 as a prognostic biomarker relevant to immune infiltrates in human esophageal squamous cell carcinoma

Contact Info

Product

Resources

About