Hemangi Shah would like to dedicate this article to her father, Shri Madhusudan Shah, to ignite a spark of creativity in her.Abstract. (M n , g) be a complete Riemannian manifold without conjugate points. In this paper, we show that if M is also simply connected, then M is flat, provided that M is also asymptotically harmonic manifold with minimal horospheres (AHM). The (first order) flatness of M is shown by using the strongest criterion: {ei} be an orthonormal basis of TpM and {be i } be the corresponding Busemann functions on M ., · · · , be n (x)), is an isometry and therefore, M is flat. Consequently, AH manifolds can have either polynomial or exponential volume growth, generalizing the corresponding result of [23] for harmonic manifolds. In case of harmonic manifold with minimal horospheres (HM), the (second order) flatness was proved in [28] by showing that span{b 2 v |v ∈ TpM } is finite dimensional. We conclude that, the results obtained in this paper are the strongest and wider in comparison to harmonic manifolds, which are known to be AH.In fact, our proof shows the more generalized result, viz.: If (M, g) is a non-compact, complete, connected Riemannian manifold of infinite injectivity radius and of subexponential volume growth, then M is a first order flat manifold.2010 Mathematics Subject Classification. Primary 53C20; Secondary 53C25, 53C35.