Mean Particle Diameters. Part VIII. Computer Program to Decompose Mixtures of (Truncated) Lognormal Particle Size Distributions Using Differential Evolution to Generate Starting Values for Nonlinear Least Squares

Alderliesten, Maarten

doi:10.1002/ppsc.201600086

Cited by 5 publications

(1 citation statement)

References 44 publications

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“…The particle sizes of materials and aerosols have been well described by log-normal distribution (Alderliesten, 2016;Finlay and Darquenne, 2020;Heintzenberg, 1994;Patterson and Gillette, 1977;Waldo et al, 1971;Yang et al, 2012). The cumulative frequency distributions of condensate sizes in pixel were fitted with a lognormal cumulative distribution function based on the F-test implemented in OriginLab.…”

Section: 𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶𝐶 𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝑓𝐶𝐶𝐶𝐶mentioning

confidence: 99%

Principles of assembly and regulation of condensates of Polycomb repressive complex 1 through phase separation

Brown

Chew

Ingersoll

et al. 2022

Preprint

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PRC1 (Polycomb repressive complex 1) plays a significant role in cellular differentiation and development by repressing lineage-inappropriate genes. PRC1 proteins phase separate to form Polycomb condensates (bodies) that are multi-component hubs for silencing Polycomb target genes; however, the molecular principles that underpin the condensate assembly and biophysical properties remain unknown. Here, by using biochemical reconstitution, cellular imaging, and multiscale molecular simulations, we show that PRC1 condensates are assembled via a scaffold-client liquid-liquid phase separation (LLPS) model by which Chromobox 2 (CBX2) is the scaffold and other subunits of the CBX2-PRC1 complex act as clients. The clients induce a reentrant phase transition of CBX2 condensates in a concentration-dependent manner. The composition of the multi-component, heterotypic LLPS systems directs the assembly and biophysical properties of CBX2-PRC1 condensates and selectively promotes the formation of CBX4-PRC1 condensates, but specifically dissolves condensates of CBX6-, CBX7-, and CBX8-PRC1. Additionally, the composition of CBX2-PRC1 condensates controls the enrichment of CBX4-, CBX7-, and CBX8-PRC1 into condensates but the exclusion of CBX6-PRC1 from condensates. Our results show the composition- and stoichiometry-dependent scaffold-client assembly of multi-component PRC1 condensates and supply a conceptual framework underlying the molecular basis and dynamics of Polycomb condensate assembly.

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