MdTFL1, a TFL1-like gene of apple, retards the transition from the vegetative to reproductive phase in transgenic Arabidopsis

Kotoda, Nobuhiro; Wada, Masato

doi:10.1016/j.plantsci.2004.07.024

Cited by 110 publications

(89 citation statements)

References 31 publications

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“…Several flowering-related genes, including LFY, TFL1, and AP1 homologs, have been isolated from grapevine (Vitis vinifera L.), citrus (Citrus sinensis Osbeck), kiwifruit (Actinidia deliciosa C. F. Liang & A. R. Ferguson), and apple (Malus domestica Borkh.) (Boss et al, 2001(Boss et al, , 2006Calonje et al, 2004;Carmona et al, 2002;Jeong et al, 1999;Joly et al, 2004;Kotoda et al, 2000Kotoda et al, , 2002Kotoda and Wada, 2005;Pillitteri et al, 2004;Sung et al, 1999Sung et al, , 2000Wada et al, 2002;Walton et al, 2001). We previously isolated and compared LFY and TFL1 homologs from six maloid fruit trees, including Japanese pear and quince, to elucidate the molecular basis of the differences in inflorescence architecture (Esumi et al, 2005); however, the derived amino acid sequences differed only slightly in their primary structures.…”

Section: Introductionmentioning

confidence: 99%

Relationship between Floral Development and Transcription Levels of LEAFY and TERMINAL FLOWER 1 Homologs in Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Esumi

Tao

Yonemori

2007

J. Japan. Soc. Hort. Sci.

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Using real-time reverse-transcription polymerase chain reaction (real-time RT-PCR) and in situ hybridization, we investigated the temporal and spatial expression of LEAFY (LFY) and TERMINAL FLOWER 1 (TFL1) homologs in Japanese pear (Pyrus pyrifolia) and quince (Cydonia oblonga) buds, in order to elucidate their roles in the flower and inflorescence development of fruit trees in the subfamily Maloideae (Rosaceae). Japanese pear and quince were selected because they are very close phylogenetically but develop distinct inflorescence architectures. Floral differentiation in Japanese pear began in late June to early July in Osaka, Japan, forming a raceme inflorescence with about eight flowers, whereas that in quince took place from late October to November in Nagano, Japan, forming a solitary flower in each floral bud. LFY homolog expression levels in both species increased at the floral differentiation stage and remained at relatively high levels in flower meristems after the flower organ differentiation stage. In contrast, TFL1 homolog expression levels in both species were high in vegetative-stage buds, but decreased significantly just before floral differentiation. Japanese pear TFL1 homologs were expressed in the subepidermal layer of the apical meristem before floral differentiation, whereas those of quince were expressed in the epidermal layer of the apical meristem and leaf primordia. We discuss the possible involvement of maloid LFY and TFL1 homologs in triggering floral differentiation, as well as the involvement of the different spatial expression patterns of TFL1 homologs in the inflorescence architectures of Japanese pear and quince.

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Section: Introductionmentioning

confidence: 99%

Relationship between Floral Development and Transcription Levels of LEAFY and TERMINAL FLOWER 1 Homologs in Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Esumi

Tao

Yonemori

2007

J. Japan. Soc. Hort. Sci.

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“…Members of the Rosaceae family have conserved orthologs of TFL1 (Chen et al 2013;Iwata et al 2012;Mimida et al 2012), which are functionally similar to TFL1. A TFL1-like gene family is found in apple (Kotoda and Wada 2005;Mimida et al 2009Mimida et al , 2012, which might function redundantly as a flowering repressor and regulator of vegetative growth. Suppression of MdTFL1 expression by antisense oligomers or gene silencing results in induction of precocious flowering in transgenic apples (Flachowsky et al 2012;Kotoda et al 2006) and simultaneously reduces vegetative growth.…”

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confidence: 99%

Overexpression of Arabidopsis FT gene in apple leads to perpetual flowering

Tanaka

Ureshino

Shigeta

et al. 2014

Plant Biotechnology

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Transgenic apple plants that overexpressed Arabidopsis FLOWERING LOCUS T (FT) under the control of the rolC promoter showed early flowering, while the introduction of FT driven by the 35S promoter induced flower development directly from transgenic apple callus in vitro, but vegetative growth was not maintained and the explant died. GFP-FT fusion proteins were detectable in transgenic apple tissues but never caused early flowering in the transformants. Under the control of the rolC promoter, the fused protein was localized in vascular tissues and fluorescence was detectable in companion cells in the stem and petiole. The transgenic apple lines that expressed AtFT driven by the rolC promoter showed differences in inflorescence architecture and floral organ number from those typical of nontransformed apple. Flowers of transgenic apple lines often contained more numerous petals, fewer stamens, and no pistils, and the pollen grains were incapable of germinating. The transgenic apple lines showed perpetual flowering without the requirement for low temperature and obvious photoperiodism. The expression patterns of six floral meristem identity genes and floral organ genes in flowers of transgenic apple lines were investigated. Among the floral meristem identity genes, expression of MdFT2 was suppressed and that of AFL2 was dramatically enhanced in the transformants. Of the floral organ genes, expression of MdMADS13, which functions as a class B gene, was unchanged from that of the flower of wild type one, whereas expression of MdMADS-NB, a possible class C gene, was suppressed.

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“…Violet. The degenerate primers containing inosine (I) used for the initial isolation were as follows: TFL-1S 5′-AAC/ TGGICAC/TGAA/GC/TTITTC/TCC-3′ and TFL-3A 5′-CG/ TC/TTGIGCA/GTTA/GAAA/GAAIAC-3′ (provided by Dr. Wada; Kotoda and Wada 2005). PCR was carried out under standard conditions using a thermal cycler (TP600, Takara Bio Inc., Ohtsu, Japan), with an initial denaturation step at 94°C for 12 min, followed by 40 cycles at 94°C for 60 s, 50°C for 60 s, and 72°C for 120 s, using AmpliTaq Gold DNA polymerase (Takara Bio, Inc.).…”

Section: Isolation Of Pntfl1 Cdnasmentioning

confidence: 99%

Effects of 9,10-ketol-octadecadienoic acid (KODA) application on single and marginal short-day induction of flowering in Pharbitis nil cv. Violet

Ono

Kataoka

Yokoyama

et al. 2013

Plant Biotechnology

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Seedlings of the Japanese morning glory (Pharbitis nil, formerly Ipomoea nil) cv. Violet are induced to flower by a single short day treatment, and endogenous levels of 9,10-ketol-octadecadienoic acid (KODA) in cotyledons were found to correlate with this mode of short-day induction. When 100 µM KODA solution was sprayed on 7-day-old seedlings before and after a marginal short-day induction, the seedlings bore approximately 4 flower buds, a one-bud increase compared with control seedlings. In treated seedlings, the second node most commonly bore the first flower bud, one node lower than in the control seedlings. We then used RT-PCR to determine expression levels of 10 genes related to photoperiodic induction of flowering. In the cotyledons, no differences were observed in expression of any genes, including P. nil FLOWERING TIME LOCUS T (PnFT1 and PnFT2), between the KODA-treated and the control seedlings. In the apical buds, P. nil APETALA1 (PnAP1) was expressed earlier in the KODA-treated seedlings than in the control seedlings. A decrease in the expression of P. nil TERMINAL FLOWER1b (PnTFL1b) was also observed in the KODA-treated seedlings. These results suggest that KODA acts as a weak enhancer of flower bud formation.

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MdTFL1, a TFL1-like gene of apple, retards the transition from the vegetative to reproductive phase in transgenic Arabidopsis

Cited by 110 publications

References 31 publications

Relationship between Floral Development and Transcription Levels of LEAFY and TERMINAL FLOWER 1 Homologs in Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Relationship between Floral Development and Transcription Levels of LEAFY and TERMINAL FLOWER 1 Homologs in Japanese Pear (Pyrus pyrifolia Nakai) and Quince (Cydonia oblonga Mill.)

Overexpression of Arabidopsis FT gene in apple leads to perpetual flowering

Effects of 9,10-ketol-octadecadienoic acid (KODA) application on single and marginal short-day induction of flowering in Pharbitis nil cv. Violet

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