MdMYB4, an R2R3-Type MYB Transcription Factor, Plays a Crucial Role in Cold and Salt Stress in Apple Calli

Wu, Ruigang; Wang, Yi; Wu, Ting; Xu, Xuefeng; Han, Zhenhai

doi:10.21273/jashs04030-17

Cited by 15 publications

(7 citation statements)

References 50 publications

(47 reference statements)

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“…During freezing stress, low temperature induces the expression of MYB transcription factors and enhances anthocyanin synthesis to enhance freezing resistance. In addition, recent investigations in apple show that MdMYB4- transgenic apple calli exhibited enhanced tolerance to cold stress, as indicated by reduced electrolyte leakage value, and MDA content than the control calli [ 69 ]. The upregulation of MYB4 may increase cold resistance in wild apples.…”

Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Zhou

Liu

et al. 2021

BMC Genomics

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Background Freezing temperatures are an abiotic stress that has a serious impact on plant growth and development in temperate regions and even threatens plant survival. The wild apple tree (Malus sieversii) needs to undergo a cold acclimation process to enhance its freezing tolerance in winter. Changes that occur at the molecular level in response to low temperatures are poorly understood in wild apple trees. Results Phytohormone and physiology profiles and transcriptome analysis were used to elaborate on the dynamic response mechanism. We determined that JA, IAA, and ABA accumulated in the cold acclimation stage and decreased during freezing stress in response to freezing stress. To elucidate the molecular mechanisms of freezing stress after cold acclimation, we employed single molecular real-time (SMRT) and RNA-seq technologies to study genome-wide expression profiles in wild apple. Using the PacBio and Illumina platform, we obtained 20.79G subreads. These reads were assembled into 61,908 transcripts, and 24,716 differentially expressed transcripts were obtained. Among them, 4410 transcripts were differentially expressed during the whole process of freezing stress, and these were examined for enrichment via GO and KEGG analyses. Pathway analysis indicated that “plant hormone signal transduction”, “starch and sucrose metabolism”, “peroxisome” and “photosynthesis” might play a vital role in wild apple responses to freezing stress. Furthermore, the transcription factors DREB1/CBF, MYC2, WRKY70, WRKY71, MYB4 and MYB88 were strongly induced during the whole stress period. Conclusions Our study presents a global survey of the transcriptome profiles of wild apple trees in dynamic response to freezing stress after two days cold acclimation and provides insights into the molecular mechanisms of freezing adaptation of wild apple plants for the first time. The study also provides valuable information for further research on the antifreezing reaction mechanism and genetic improvement of M. sieversii after cold acclimation.

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Section: Discussionmentioning

confidence: 99%

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Zhou

Liu

et al. 2021

BMC Genomics

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“…The fragments were then cloned into the pCambia super 1300 vector between Sal I and Kpn I restriction sites to generate B1, B2, and B3 constructs. The resulting recombinant vector was inserted into A. tumefaciens LBA4404 cells, which were then used to transform ‘Orin’ callus cells for 20 min, as described in Wu, Wang, Wu, Xu, and Han (). The cells were cocultured on agar‐solidified MS medium without antibiotics in darkness at 25°C for 24 hr then transferred to screening medium for 30 days to select for transformed cells.…”

Section: Methodsmentioning

confidence: 99%

Natural variation in cytokinin maintenance improves salt tolerance in apple rootstocks

Feng

Liu

Zhai

et al. 2018

Plant Cell & Environment

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Plants experiencing salt-induced stress often reduce cytokinin levels during the early phases of stress-response. Interestingly, we found that the cytokinin content in the apple rootstock "robusta" was maintained at a high level under salt stress. Through screening genes involved in cytokinin biosynthesis and catabolism, we found that the high expression levels of IPT5b in robusta roots were involved in maintaining the high cytokinin content. We identified a 42 bp deletion in the promoter region of IPT5b, which elevated IPT5b expression levels, and this deletion was linked to salt tolerance in robusta×M.9 segregating population. The 42 bp deletion resulted in the deletion of a Proline Response Element (ProRE), and our results suggest that ProRE negatively regulates IPT5b expression in response to proline. Under salt stress, the robusta cultivar maintains high cytokinin levels as IPT5b expression cannot be inhibited by proline due to the deletion of ProRE, leading to improve salt tolerance.

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“…Studies have shown that MeJA can activate antioxidant metabolic pathways and defense mechanisms in various crops and enhance cold tolerance [10]. In addition, some members of ERF, bHLH, MYB, DREB, and WRKY transcription factor families, such as ERF53 [11], ICE1, DREB1A [12], MYB4 [13], and WRKY19 [14], are also involved in regulating the expression of cold-stress response genes.…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis and differential gene expression profiling of wucai (Brassica campestris L.) in response to cold stress

et al. 2022

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Background Wucai suffers from low temperature during the growth period, resulting in a decline in yield and poor quality. But the molecular mechanisms of cold tolerance in wucai are still unclear. Results According to the phenotypes and physiological indexes, we screened out the cold-tolerant genotype “W18” (named CT) and cold-sensitive genotype “Sw-1” (named CS) in six wucai genotypes. We performed transcriptomic analysis using seedling leaves after 24 h of cold treatment. A total of 3536 and 3887 differentially expressed genes (DEGs) were identified between the low temperature (LT) and control (NT) comparative transcriptome in CT and CS, respectively, with 1690 DEGs specific to CT. The gene ontology (GO) analysis showed that the response to cadmium ion (GO:0,046,686), response to jasmonic acid (GO:0,009,753), and response to wounding (GO:0,009,611) were enriched in CT (LT vs NT). The DEGs were enriched in starch and sucrose metabolism and glutathione metabolism in both groups, and α-linolenic acid metabolism was enriched only in CT (LT vs NT). DEGs in these processes, including glutathione S-transferases (GSTs), 13S lipoxygenase (LOX), and jasmonate ZIM-domain (JAZ), as well as transcription factors (TFs), such as the ethylene-responsive transcription factor 53 (ERF53), basic helix-loop-helix 92 (bHLH92), WRKY53, and WRKY54.We hypothesize that these genes play important roles in the response to cold stress in this species. Conclusions Our data for wucai is consistent with previous studies that suggest starch and sucrose metabolism increased the content of osmotic substances, and the glutathione metabolism pathway enhance the active oxygen scavenging. These two pathways may participated in response to cold stress. In addition, the activation of α-linolenic acid metabolism may promote the synthesis of methyl jasmonate (MeJA), which might also play a role in the cold tolerance of wucai.

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MdMYB4, an R2R3-Type MYB Transcription Factor, Plays a Crucial Role in Cold and Salt Stress in Apple Calli

Cited by 15 publications

References 50 publications

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Transcriptome profiling of Malus sieversii under freezing stress after being cold-acclimated

Natural variation in cytokinin maintenance improves salt tolerance in apple rootstocks

Transcriptome analysis and differential gene expression profiling of wucai (Brassica campestris L.) in response to cold stress

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