mdm2 mRNA Level is a Prognostic Factor in Soft Tissue Sarcoma

Täubert, Helge; Kœhler, Thomas; Meye, Axel; Bartel, Frank; Lautenschläger, C; Borchert, Silke; Bache, Matthias; Schmidt, Hannelore; Würl, Peter

doi:10.1007/bf03401934

Cited by 24 publications

(18 citation statements)

References 53 publications

(60 reference statements)

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“…Despite the importance of p53 in the regula- tion of these vital cell functions, and the fact that mutated p53 has been shown to be of prognostic importance in other forms of cancer, the prognostic importance of p53 in STS is controversial [30,67,160]. Amplification of mdm2 is an especially important mechanism leading to p53 inactivation in STS, and some studies suggest that a possible prognostic importance of p53 may depend on coexpression of mdm2 [167,184,186]. However, the great number of studies that have been performed without reaching an answer as to a prognostic role of p53 in STS indicate that a possible prognostic importance of p53 immunostaining is small.…”

Section: P53 Mdm2 and Bcl-2mentioning

confidence: 99%

Prognostic factors in soft tissue sarcomaTissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence

Engellau¹

2004

Acta Orthopaedica Scandinavica

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Section: P53 Mdm2 and Bcl-2mentioning

confidence: 99%

Prognostic factors in soft tissue sarcomaTissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence

Engellau¹

2004

Acta Orthopaedica Scandinavica

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“…The repeatability of the Roboscreen assays was good: the coefficients of variation of the mean reference curves slopes were usually below 5% which indicates reliable standard stability. 21,29 The precision of the assays was tested by analysis of cDNAs prepared from the K562 and CCRF ADR5000 cell lines for Bcl-2 and GAPDH expression, respectively. Input analyte amounts at the attomole detection level, ie typical for GAPDH reference transcripts, were detected within a 10% precision rate, whereas Bcl-2 transcripts which are usually at a low zeptomole detection level were detected within a precision of ±28% (eight repeated analyses performed for each cell line, data not shown).…”

Section: Validation Of Quantitative Rt-pcrmentioning

confidence: 99%

“…19,20 We recently showed that low levels of Mdm-2 mRNA are associated with poor prognosis in soft tissue sarcoma. 21 Several attempts have been made to correlate expression levels of resistance genes with clinical outcome of leukemia. 14,[22][23][24][25] Only a few studies have assessed the association between candidate gene expression and survival in acute myelogenous leukemia (AML), most of them with rather small patient numbers, and sometimes with conflicting results.…”

Section: Introductionmentioning

confidence: 99%

High Bad and Bax mRNA expression correlate with negative outcome in acute myeloid leukemia (AML)

et al. 2002

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The search for molecular markers in AML that allow prediction of outcome has recently focused on genes involved in the regulation of programmed cell death (PCD). The aim of our study was to determine whether mRNA levels of Mdm-2, Bcl-2, Bclx L , Bad, and Bax are independent prognostic parameters for outcome. Transcript levels were analyzed by real-time quantitative RT-PCR in 232 samples collected either at diagnosis or following induction chemotherapy (ICT). Multivariate COX regression analysis adjusted for chemotherapy protocol, de novo vs secondary AML, and de novo vs relapsed AML indicated: (1) At diagnosis, high expression of Bad (P ‫؍‬ 0.015) and even more so high Bax and Bad levels (P ‫؍‬ 0.018) predicted adverse outcome, regardless of the response to ICT. In patients who subsequently failed to enter complete remission (CR), high levels of Bad, Bax and Bax high/Bad high were associated with an increased relative risk (RR) to die from tumor (RR ‫؍‬ 5.0 for Bad, 3.4 for Bax and 6.14 for Bax high/Bad high). (2) Following ICT, high expression of Bax (P ‫؍‬ 0.005) and high Bcl-2/Bax ratios (P ‫؍‬ 0.004) were independent predictors of unfavorable outcome, regardless of response to ICT. We conclude that high levels of Bax and Bad correlate with poor outcome, particularly in patients who do not enter CR and may serve as prognostic markers in AML.

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“…Godfrey et al 12 have shown that sensitive and linear RNA transcript measurements can be obtained from formalinfixed paraffin embedded clinical samples despite significant RNA degradation, as long as the amplicon size is Ͻ130 bases.We chose to validate performance of the TaqMan assay in our clinical application (cells scraped from the cervix) using ␤-GUS and GAPDH genes, as they are well accepted as internal standards [13][14][15] and have been optimized for this assay. 12 To test the effect of degraded RNA on the RT efficiency, GAPDH was amplified from a pooled clinical sample equally divided and extracted in parallel by RNeasy and Trizol/phenol-chloroform procedures.…”

Section: Measurement Of Housekeeping Genesmentioning

confidence: 99%

Measurements of Human Papillomavirus Transcripts by Real Time Quantitative Reverse Transcription-Polymerase Chain Reaction in Samples Collected for Cervical Cancer Screening

Lamarcq

Deeds

Ginzinger³

et al. 2002

The Journal of Molecular Diagnostics

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Specific assays capable of distinguishing normal and atypical cervical changes from pre-cancerous lesions are direly needed to improve screening for cervical cancer. Specific genes transcripts that are up-regulated in dysplastic and cancer cells can be exploited as new markers for cervical cancer screening provided that they can be detected in heterogeneous populations such as those collected for Papanicolaou tests. We hypothesized that expression of the HPV early region gene E7 might distinguish between normal samples (absent expression) and high-grade lesions (detectable E7 expression). Our goal was to detect and measure gene expression in cells scraped from the cervix using real time quantitative reverse transcription-polymerase chain reaction (TaqMan). We have optimized collection and extraction procedures to provide suitable RNA for TaqMan analysis in clinical samples collected for cervical cancer screening and have demonstrated efficient measurements of housekeeping genes in these samples. HPV 16 or 18 early gene E7 transcripts were detected in 47% of samples with a clinical diagnosis of high-grade SIL and in 0% of cytologically normal samples (P ‫؍‬ 0.006). Our study demonstrates that the TaqMan assay can be reliably applied to samples collected for cervical cancer screening, and that presence of detectable HPV E7 transcripts can distinguish between normal and abnormal samples. World-wide, cervical cancer is the leading cause of cancer morbidity in women.

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mdm2 mRNA Level is a Prognostic Factor in Soft Tissue Sarcoma

Cited by 24 publications

References 53 publications

Prognostic factors in soft tissue sarcomaTissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence

Prognostic factors in soft tissue sarcomaTissue microarray for immunostaining, the importance of whole-tumor sections and time-dependence

High Bad and Bax mRNA expression correlate with negative outcome in acute myeloid leukemia (AML)

Measurements of Human Papillomavirus Transcripts by Real Time Quantitative Reverse Transcription-Polymerase Chain Reaction in Samples Collected for Cervical Cancer Screening

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