MdKNOX15, a class I knotted-like transcription factor of apple, controls flowering and plant height by regulating GA levels through promoting the MdGA2ox7 transcription

Jia, Peng; Xing, Libo; Zhang, Chenguang; Chen, Hao; Li, Youmei; Zhang, Dong; Ma, Junsheng; Zhao, Caiping; Han, Mingyu; Ren, Xiaolin; An, Na

doi:10.1016/j.envexpbot.2021.104411

Cited by 22 publications

(13 citation statements)

References 53 publications

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“…Intriguingly, verification of subcellular locations of AsKNOX6 and AsKNOX8 showed that AsKNOX6 was consistently localized in the nucleus, while AsKNOX8 was unexpectedly localized on the cytoplasm (Figure 8). The subcellular localization of KNOX proteins outside the nucleus was also found in other plant species, such as the MdKNOX15 in apple located in both the nucleus and plasma membrane [28] and the KNAT1 in Arabidopsis when co-expressed with AtOFP1 or AtOFP5, in which the AtOFP negatively controls the activity of KNOX-BELL protein dimers by changing their localization from the nucleus to the cytoplasmic space [29]. Transcription factors might be retained outside the nucleus for several potential reasons, for examples containing membrane-binding domains, interacting with protein partners that inhibit nuclear import or directly interplaying with membranes, and the posttranslational modifications (phosphorylation, acetylation, glycosylation, ubiquitination, sumoylation, and redox-dependent changes) [30].…”

Section: Discussionsupporting

confidence: 56%

Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic (Allium sativum L.) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin

Zhang

Pan

Zhi

et al. 2021

IJMS

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Garlic (Allium sativum L.) is an important vegetable and is cultivated and consumed worldwide for its economic and medicinal values. Garlic cloves, the major reproductive and edible organs, are derived from the axillary meristems. KNOTTED-like homeobox (KNOX) proteins, such as SHOOT MERISTEM-LESS (STM), play important roles in axillary meristem formation and development. However, the KNOX proteins in garlic are still poorly known. Here, 10 AsKNOX genes, scattered on 5 of the 8 chromosomes, were genome-wide identified and characterized based on the newly released garlic genome. The typical conserved domains of KNOX proteins were owned by all these 10 AsKNOX homologs, which were divided into two Classes (Class I and Class II) based on the phylogenetic analysis. Prediction and verification of the subcellular localizations revealed the diverse subcellular localization of these 10 AsKNOX proteins. Cis-element prediction, tissue expression analysis, and expression profilings in responding to exogenous GA3 and 6-BA showed the potential involvement of AsKNOX genes in the gibberellin and cytokinin signaling pathways. Overall, the results of this work provided a better understanding of AsKNOX genes in garlic and laid an important foundation for their further functional studies.

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Section: Discussionsupporting

confidence: 56%

Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic (Allium sativum L.) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin

Zhang

Pan

Zhi

et al. 2021

IJMS

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“…Although some studies have reported KNOX I was localized on nucleus, it was also found that KNOX was localized on the cell membrane except the nucleus. 32–37 Therefore, the subcellular localization pattern is different in species. In addition, the subcellular localization of the LtKNOX1 protein may be affected by intercellular transport.…”

Section: Discussionmentioning

confidence: 99%

Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Zhou

Fan

Zhao

et al. 2022

Plant Signaling & Behavior

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Leaves are the main vegetative organs of the aboveground part of plants and play an important role in plant morphogenesis. KNOTTED-LIKE HOMEOBOX ( KNOX ) plays a crucial role in regulating leaf cell fate and maintaining leaf development. In this study, we analyzed LtKNOX1 from Lilium tsingtauense and illustrated its function in transgenic plants. Tissue-specific expression analysis indicated that LtKNOX1 was highly expressed in stems, young flower buds, and shoot apical meristems (SAMs). Ectopic overexpression of LtKNOX1 in Nicotiana benthamiana suggested that transformants with mild phenotypes were characterized by foliar wrinkles and mildly curled leaves; transformants with intermediate phenotypes showed severely crimped blades and narrow leaf angles, and the most severe phenotypes lacked normal SAMs and leaves. Moreover, the expression levels of genes involved in the regulation of KNOX in transgenic plants were detected, including ASYMMETRIC LEAVES1, PIN-FORMED 1, GA20-oxidase, CUP-SHAPED COTYLEDON 2, CLAVATA 1 and WUSCHEL ( WUS ), and the expression of other genes were down-regulated except WUS . This study contributes to our understanding of the LtKNOX1 function.

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“…The transformants were identified based on kanamycin resistance, and the T 3 generation seedlings were used for functional analyses. The flowering phenotype of different Arabidopsis lines, including JrAHL2 -overexpressing ( JrAHL2-OE ), wild type (Col-0), and AtAHL22 mutant ( ahl22-1 , Salk_018866), were identified according to the previous description [ 58 ]. To analyze the hypocotyl growth phenotype, Arabidopsis seeds were surface-sterilized and sown on Murashige & Skoog (MS) medium [ 56 ].…”

Section: Methodsmentioning

confidence: 99%

Systematical Characterization of the AT-Hook Gene Family in Juglans regia L. and the Functional Analysis of the JrAHL2 in Flower Induction and Hypocotyl Elongation

Jia

Liu

Yan

et al. 2023

IJMS

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AT-hook motif nuclear localization (AHL) proteins play essential roles in various plant biological processes. Yet, a comprehensive understanding of AHL transcription factors in walnut (Juglans regia L.) is missing. In this study, 37 AHL gene family members were first identified in the walnut genome. Based on the evolutionary analysis, JrAHL genes were grouped into two clades, and their expansion may occur due to segmental duplication. The stress-responsive nature and driving of developmental activities of JrAHL genes were revealed by cis-acting elements and transcriptomic data, respectively. Tissue-specific expression analysis showed that JrAHLs had a profound transcription in flower and shoot tip, JrAHL2 in particular. Subcellular localization showed that JrAHL2 is anchored to the nucleus. Overexpression of JrAHL2 in Arabidopsis adversely affected hypocotyl elongation and delayed flowering. Our study, for the first time, presented a detailed analysis of JrAHL genes in walnut and provided theoretical knowledge for future genetic breeding programs.

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MdKNOX15, a class I knotted-like transcription factor of apple, controls flowering and plant height by regulating GA levels through promoting the MdGA2ox7 transcription

Cited by 22 publications

References 53 publications

Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic (Allium sativum L.) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin

Genome-Wide Identification and Characterization of KNOTTED-Like Homeobox (KNOX) Homologs in Garlic (Allium sativum L.) and Their Expression Profilings Responding to Exogenous Cytokinin and Gibberellin

Overexpression of LtKNOX1 from Lilium tsingtauense in Nicotiana benthamiana affects the development of leaf morphology

Systematical Characterization of the AT-Hook Gene Family in Juglans regia L. and the Functional Analysis of the JrAHL2 in Flower Induction and Hypocotyl Elongation

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