MdBZR1 regulates ABA response by modulating the expression of MdABI5 in apple

et al. 2022

J. Agric. Food Chem.

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Hydrogen peroxide (H2O2), as a main component of reactive oxygen species (ROS), serves as a key signaling molecule relevant to plant stress response and health status. Many strategies have been developed for detecting or quantifying H2O2 concentration. However, reports on simply, visibly tracking H2O2 fluctuation in vivo are limited. Here, for visibly tracking the plant H2O2 wave, a green fluorescent phenotypic probe was designed by merging a H2O2-sensitive tertiary amine moiety with the core fluorophore tetraphenylethene skeleton. The green fluorescence emission is quenched up to 52% by H2O2 with good sensitivity, selectivity, and reversibility within the plant physiological range of 10–100 μM H2O2. In response to various abiotic stresses, including mechanical damage, high salt, strong light and drought, fluorescence fluctuations, response to H2O2 concentration alterations in vivo was visible to the naked eye under irradiation of commercially available UV light (365 nm) after simple injection of this H2O2 probe solution into seedling leaves. This phenotypic fluorescent H2O2 probe illustrates great potential as early sensors of plant health under stress without the aid of skillful operation and specialized equipment.

Section: Resultsmentioning

confidence: 99%

Simple Phenotypic Sensor for Visibly Tracking H₂O₂ Fluctuation to Detect Plant Health Status

Yin

Wang

et al. 2022

J. Agric. Food Chem.

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“…All data analysis was based on three biological replicates, and signifcant differences among treatments were assessed by using the DPS software as described previously (Liu et al, 2021)…”

Section: Discussionmentioning

confidence: 99%

“…It has been verified that MdABI5 positively regulates ABA signalling and is sensitive to ABA (Liu et al, 2021). We wondered whether MdTCP46 directly interacts with MdABI5, and Y2H assays…”

Section: Mdtcp46 Directly Interacts With Mdabi5mentioning

confidence: 97%

MdTCP46 interacts with MdABI5 to negatively regulate ABA signalling and drought response in apple

Plant Cell & Environment

Gao

et al. 2022

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TEOSINTE BRANCHED 1/CYCLOIDEA/PCF (TCP) transcription factors play crucial roles in plant abiotic stresses. However, little is known about the role of TCP genes in the drought stress tolerance of apple. Here, we found that abscisic acid (ABA) and drought treatment reduced the expression of MdTCP46, and overexpression of MdTCP46 reduced ABA sensitivity and drought stress resistance. MdTCP46 was found to interact with MdABI5 both in vitro and in vivo, and this interaction was essential for drought resistance via the ABA-dependent pathway. Overexpression of MdABI5 enhanced ABA sensitivity and drought stress resistance by directly activating the expression of MdEM6 and MdRD29A. MdTCP46 significantly suppressed the transcriptional activity of MdABI5, thereby negatively regulating MdABI5-mediated ABA signalling and drought response. Overall, our results demonstrate that the MdTCP46-MdABI5-MdEM6/MdRD29A regulatory module plays a key role in the modulation of ABA signalling and the drought stress response. These findings provide new insight into the role of MdTCP46 in ABA signalling and abiotic stress responses.

“…RNA extraction and reverse transcription were carried out as described by Liu et al 30 . Quantitative real-time polymerase chain reaction (qRT-PCR) was performed using Step One Plus.…”

Section: Methodsmentioning

confidence: 99%

The MdABI5 transcription factor interacts with the MdNRT1.5/MdNPF7.3 promoter to fine-tune nitrate transport from roots to shoots in apple

Gao

et al. 2021

Hortic Res

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Nitrate is a major nitrogen resource for plant growth and development and acts as both a crucial nutrient and a signaling molecule for plants; hence, understanding nitrate signaling is important for crop production. Abscisic acid (ABA) has been demonstrated to be involved in nitrate signaling, but the underlying mechanism is largely unknown in apple. In this study, we found that exogenous ABA inhibited the transport of nitrate from roots to shoots in apple, and the transcription of the nitrate transporter MdNRT1.5/MdNPF7.3 was noticeably reduced at the transcriptional level by ABA, which inhibited the transport of nitrate from roots to shoots. Then, it was found that the ABA-responsive transcription factor MdABI5 bound directly to the ABRE recognition site of the MdNRT1.5 promoter and suppressed its expression. Overexpression of MdABI5 inhibited ABA-mediated transport of nitrate from roots to shoots. Overall, these results demonstrate that MdABI5 regulates the transport of nitrate from roots to shoots partially by mediating the expression of MdNRT1.5, illuminating the molecular mechanism by which ABA regulates nitrate transport in apple.