MDA-468, a human breast cancer cell line with a high number of epidermal growth factor (EGF) receptors, has an amplified EGF receptor gene and is growth inhibited by EGF

Abstract

Epidermal growth factor (EGF) has been noted to stimulate prolifereation of a variety of normal and malignant cells including those of human breast epithelium. We report here that MDA-468, a human breast cancer cell line with a very high number of EGF receptors, is growth-inhibited at EGF concentrations that stimulate most other cells. The basis for the elevated receptor level is EGF receptor gene amplification and over-expression. An MDA-468 clone selected for resistance to EGF-induced growth inhibition shows a number of receptors within the normal range. The results are discussed in relation to a threshold model for EGF-induced growth inhibition

Growth inhibition of breast cancer cells by Grb2 downregulation is correlated with inactivation of mitogen-activated protein kinase in EGFR, but not in ErbB2, cells

“…MDA-MB-468 cells grew faster in the presence of 10 versus 0.5% FCS, and their growth could be modulated upon addition of exogenous EGF. Low doses of EGF stimulate MDA-MB-468 cell growth while high doses of EGF are growth-inhibitory (Filmus et al, 1985). Under our culture conditions, EGF did not inhibit the growth of MDA-MB-468 cells.…”

“…Liposomal Grb2 antisense oligos (L-Grb2) inhibited the proliferation of EGFR-overexpressing breast cancer cells in an EGF-dependent manner MDA-MB-468 cells overexpress EGFR as a result of gene ampli®cation (Filmus et al, 1985). These cells do not need any exogenous growth factors to grow when cultured in the presence of fetal calf serum (FCS).…”

“…These cells do not need any exogenous growth factors to grow when cultured in the presence of fetal calf serum (FCS). However, their growth rate may be modulated by the amount of serum or growth factors used (Filmus et al, 1985). MDA-MB-468 cells grew faster in the presence of 10 versus 0.5% FCS, and their growth could be modulated upon addition of exogenous EGF.…”

Epiregulin binds to epidermal growth factor receptor and ErbB-4 and induces tryosine phosphorylation of epidermal growth factor receptor, ErbB-2, ErbB-3 and ErbB-4

“…The MDA-MB-468 cell line expresses a high level of EGFR (Filmus et al, 1985) and a moderate level of ErbB-3 protein (Kraus et al, 1987;Alimandi et al, 1995), but does not express of ErbB-2 (Lupu et al, 1990;Hancock et al, 1991) or ErbB-4 mRNA (Plowman et al, 1993a). The SK-BR-3 cell line expresses EGFR, ErbB-2 (Kraus et al, 1987) and ErbB-3 (Alimandi et al, 1995) but not ErbB-4 mRNA (Plowman et al, 1993a).…”

Analysis of EGF receptor amplicons reveals amplification of multiple expressed sequences

“…Cell lines MDA468 and A431, both of which stably maintain ampli®ed EGFR (Merlino et al, 1984;Filmus et al, 1985), and cell lines U138 and U87 were obtained from American Type Culture Collection.…”