MCP-1-Induced Protein Promotes Endothelial-Like and Angiogenic Properties in Human Bone Marrow Monocytic Cells

Abstract

Monocytic cells enhance neovascularization by releasing proangiogenic mediators and/or by transdifferentiating into endothelial-like cells. However, the mechanisms that govern this transdifferentiation process are largely unknown. Recently, monocyte chemotactic protein-1 (MCP-1)-induced protein (MCPIP) has been identified as a novel CCCH-type zinc-finger protein expressed primarily in monocytic cells. Here, we analyzed whether MCPIP might exert angiogenic effects by promoting differentiation of monocytic cells into endothelial cell ( These results demonstrate that MCPIP may be an important regulator of inflammatory angiogenesis and provide novel mechanistic insights into the link between MCP-1 and cardiovascular diseases.

MCP-1-induced protein attenuates post-infarct cardiac remodeling and dysfunction through mitigating NF-κB activation and suppressing inflammation-associated microRNA expression

“…Cells with central nuclei as judged by eye were chosen for measurements. Cell death by apoptosis was assessed by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) using the In Situ Cell Death Detection Kit, TMR red (Roche Diagnostics) as described previously [44]. Then, the slides were washed in PBS and stained with anti-sarcomeric a-actin antibody (Abcam Inc.) overnight at 4°C followed by incubation with an FITC-conjugated secondary antibody (Chemicon International) in the dark at room temperature.…”

“…Myocardial cytosolic and nuclear proteins were extracted from mice myocardium with ice-cold tissue lysis buffer as we described previously [44]. Equal amounts of proteins were run on 12 % SDS-PAGE and transferred to nitrocellulose membranes.…”

“…The total TUNEL-positive cells were calculated in five randomly selected fields for each animal, and six to eight animals were studied per group. The activity of caspase-3/7 was also determined using the Apo-ONE homogenous caspase-3/7 Assay kit (Promega) as described previously [44].…”

“…All samples were run in duplicate. Relative expression was calculated using the comparative threshold cycle (Ct) method as we described previously [44].…”

“…Monocyte chemoattractant protein-1 (MCP-1)-induced protein (MCPIP, also known as ZC3H12A or Regnase-1) was first identified as a ZCCCH-zinc finger protein which manifests a transcription factor-like activity [59], and regulates several biological function such as angiogenesis [39,44,47] and adipogenesis [57]. MCPIP is primarily expressed in monocytes and macrophages, and its transcription is rapidly induced in various types of cells upon NF-jB activation [49].…”