MCM interference during licensing of DNA replication in<i>Xenopus</i>egg extracts-Possible Role of a C-terminal region of MCM3-

Mimura, Satoru; Kubota, Yumiko; Takisawa, Haruhiko

doi:10.1080/15384101.2017.1415681

Cited by 5 publications

(5 citation statements)

References 48 publications

(77 reference statements)

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“…Licensing of replication origins by the human MCM-DH has been implicated from studies of other vertebrates [17][18][19][20][21][22] . Indeed, most of our assumed knowledge about the molecular mechanisms of replication initiation in human are inferred from studies in yeast and other metazoans 1,2,[23][24][25] .…”

Section: Introductionmentioning

confidence: 99%

The human pre-replication complex is an open complex

Dong

Wang

et al. 2023

Cell

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Section: Introductionmentioning

confidence: 99%

The human pre-replication complex is an open complex

Dong

Wang

et al. 2023

Cell

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“…We identified four DNA replication licensing factors involved in minichromosome maintenance (MCM 2, 3, 4, and 7) from among a total of six high-connectivity degree (>10) DEPs (Additional file 24: Table S15). The DNA replication licensing factor MCM complex, consisting of six subunits, MCM 2–7, is loaded onto the replication origins through loading factors (origin recognition complex [ORC], Cdc6, and Cdt1) and forms an MCM double hexamer that licenses the initiation of DNA replication [42]. The functions of MCM have been mainly studied in medical science, especially in cancer [43].…”

Section: Discussionmentioning

confidence: 99%

Phenotypic analysis combined with tandem mass tags (TMT) labeling reveal the heterogeneity of strawberry stolon buds

et al. 2019

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BackgroundRamet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds.ResultsFrom observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB), and the new active axillary stolon buds (ASB) differentiated continually after the differentiation of the first leaf. Using the tandem mass tags (TMT) labeling method, a total of 7271 strawberry proteins were identified. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A and PK) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change DEP of LSH 10-like might be involved in the degeneration of SAM into RLBs, based on its significant function in modulating the plant shoot initiation. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)].ConclusionsBy using phenotypic observation combined with proteomic networks with different types of strawberry stolon buds, the definite dormancy phase of DSB was identified, and the biological pathways and gene networks that might be responsible for heterogeneity among different stolon buds in strawberry were also revealed.

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“…We identified four DNA replication licensing factors involved in minichromosome maintenance (MCM 2, 3, 4, and 7) from among a total of six high-connectivity degree (>10) DEPs (supplemental Table S15). The DNA replication licensing factor MCM complex, consisting of six subunits, MCM 2-7, is loaded onto the replication origins through loading factors (origin recognition complex [ORC], Cdc6, and Cdt1) and forms an MCM double hexamer that licenses the initiation of DNA replication [42]. The functions of MCM have been mainly studied in medical science, especially in cancer [43].…”

Section: Tmt Revealed the Heterogeneity Of Stolon Buds In Strawberrymentioning

confidence: 99%

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds

Guan

Zhao²,

Qian

et al. 2019

Preprint

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Background: Ramet propagation in strawberry (Fragaria × ananassa) is the most effective way in production. However, the lack of systematically phenotypic observations and high-throughput methods limits our ability to analyze the key factors regulating the heterogeneity in strawberry stolon buds. Results: From observation, we found that the axillary bud located in the first node quickly stepped into dormancy (DSB), after several bract and leaf buds were differentiated. The stolon apical meristem (SAM) degenerated as the new ramet leaf buds (RLB), and the new active axillary stolon buds (ASB) differentiated continually after the differentiation of the first leaf. Using the tandem mass tags (TMT) labeling method, a total of 7,271 strawberry proteins were identified. Between ASB and DSB, the spliceosome DEPs, such as Ser/Arg-rich (SR) and heterogeneous nuclear ribonucleoprotein particle (hnRNP), showed the highest enrichment and high PPI connectivity. This indicated that the differences in DEPs (e.g., SF-3A and PK) at the transcriptional level may be causing the differences between the physiological statuses of ASB and DSB. As expected, the photosynthetic pre-form RLB mainly differentiated from ASB and DSB judging by the DEP enrichment of photosynthesis. However, there are still other specialized features of DEPs between RLB and DSB and between ASB and DSB. The DEPs relative to DNA duplication [e.g., minichromosome maintenance protein (MCM 2, 3, 4, 7)], provide a strong hint of functional gene duplication leading the bud heterogeneity between RLB and DSB. In addition, the top fold change DEP of LSH 10-like might be involved in the degeneration of SAM into RLBs, based on its significant function in modulating the plant shoot initiation. As for RLB/ASB, the phenylpropanoid biosynthesis pathway probably regulates the ramet axillary bud specialization, and further promotes the differentiation of xylem when ASB develops into a new stolon [e.g., cinnamyl alcohol dehydrogenase 1 (CAD1) and phenylalanine ammonia-lyase 1 (PAL1)]. Conclusions: By using phenotypic observation combined with proteomic networks with different types of strawberry stolon buds, the definite dormancy phase of DSB was identified, and the biological pathways and gene networks that might be responsible for heterogeneity among different stolon buds in strawberry were also revealed.

show abstract

MCM interference during licensing of DNA replication inXenopusegg extracts-Possible Role of a C-terminal region of MCM3-

Cited by 5 publications

References 48 publications

The human pre-replication complex is an open complex

The human pre-replication complex is an open complex

Phenotypic analysis combined with tandem mass tags (TMT) labeling reveal the heterogeneity of strawberry stolon buds

Phenotypic Analysis Combined with Tandem Mass Tags (TMT) Labeling Reveal the Heterogeneity of Strawberry Stolon Buds

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