MceG stabilizes the Mce1 and Mce4 transporters in Mycobacterium tuberculosis

Fieweger, Rachael A; Wilburn, Kaley M; Montague, Christine R; Roszkowski, Emma K; Kelly, Carolyn M.; Southard, Teresa; Sondermann, Holger; Nazarova, Evgeniya V.; VanderVen, Brian C.

doi:10.1016/j.jbc.2023.102910

Cited by 4 publications

(3 citation statements)

References 56 publications

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“…It is reported that the Mce1 and Mce4 transporters are consist of subunits that provide substrate specificity and proteins that couple lipid transport to ATP hydrolysis. Therefore, potent chemical inhibitors of Mce-family lipoprotein would be predicted to block M. tuberculosis capability to use various key lipid nutrients concurrently while negatively impacting bacterial fitness, which may improve antibiotic treatment options for tuberculosis 38 .…”

Section: Discussionmentioning

confidence: 99%

A potent subset of Mycobacterium tuberculosis glycoproteins as relevant candidates for vaccine and therapeutic target

Yari,

Afrough,

Yari

et al. 2023

Sci Rep

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Tuberculosis (TB) remains one of the most afflictive bacterial infections globally. In high burden TB countries, surveillance, diagnosis and treatment of drug resistant TB (RR and X/MDRTB) display a crucial public health challenge. Therefore, we need new TB vaccines; diagnostic and therapeutic strategies to briskly prevent disease promotion; reduce drug-resistant TB and protect everyone from disease. The study identified various potent membrane and cell wall M. tuberculosis glycolipoproteins that are relevant for diagnostics, drug and vaccine discovery. A M. tuberculosis Proskauer and Beck broth culture was extracted for total proteins by ammonium sulfate method. After ConA-Affinity Chromatography reputed glycoproteins were collected followed by 2DE gel electrophoresis and LC Mass spectrometry. A total of 293 glycoproteins were identified using GlycoPP and IEDB database. Probable conserved trans-membrane protein (Rv0954), LpqN (Rv0583), PPE68 (Rv3873), Phosphate-binding protein (Rv0932c), PPE61 (Rv3532) and LprA (Rv1270c), had the highest glycosylation percentage value with 13.86%, 11.84%, 11.68%, 11.1%, 10.59% and10.2%, respectively. Our study discloses several dominant glycoproteins that play roles in M. tuberculosis survival, and immunogenicity. These include glycoproteins involved in antigenicity, transport and biosynthesis of M. tuberculosis cell envelope, pathogen-host interaction and drug efflux pumps, which are considered as a feasible drug targets or TB new vaccine candidates.

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Section: Discussionmentioning

confidence: 99%

A potent subset of Mycobacterium tuberculosis glycoproteins as relevant candidates for vaccine and therapeutic target

Yari,

Afrough,

Yari

et al. 2023

Sci Rep

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“…The metabolism of cholesterol requires the activity of many oxygenates and is probably an explanation of why MTB would infect the lungs, where large concentrations of oxygen are found. The mce 4 operon has been shown to be responsible for encoding a cholesterol transport system and host membrane lipid uptake, in addition to participating in energy pathways from these lipids, which allows the infection and permanence of the bacilli by long periods of time [102][103][104][105][106][107][108][109][110][111][112].…”

Section: A Complex Metabolism For Survive and Infectmentioning

confidence: 99%

“…The MTB presents membrane proteins important for the transport of several substrates, these transport systems correspond to the four main superfamilies: ATP Binding Cassette (ABC) that are considered primary transporters, since they use ATP as energy source for the system; Small Multidrug Resistance (SMR), Resistance Nodulation Division (RND), Major Facilitator Super-family (MFS), which are considered secondary transporters because they use the proton motor force (H+ or Na+) as energy. The Multidrug and Toxic-compound Extrusion (MATE) family is another important superfamily of transporters but not reported in MTB [103,[136][137][138][139][140][141].…”

Section: Transport Substrates Across the Membrane: The Set Transporte...mentioning

confidence: 99%

The <em>Mycobacterium tuberculosis</em>: A Silent Killer

Oliveira,

Ferreira,

Matta

et al. 2023

Preprint

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Mycobacterium tuberculosis is the agent of tuberculosis, one of the most important infectious diseases in the world. This microorganism stands out from other bacteria, not only for its extremely high infection capacity, but also for its cellular characteristics that include an extremely resistant and hydrophobic cellular parade the passage of antibiotics. An incredible ability to adapt to adverse conditions inside the host as well as its vast arsenal of virulence factors that allow its survival within the inhospitable environment within the macrophage can be highlighted. This review aims to discuss several aspects of MTB microbiology, genetics, and physiology. We will address in this review details of the metabolism of MTB that allows it to replicate in the active phase and remain viable during latency, as well as the characteristics of its cell wall that contribute to the blockade of the immune response and its resistance to antibiotics.

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