MCA1 detection of Histone H3 serine 10 phosphorylation, a novel biomarker for determination of mitotic indices

Rayzman, Veronika; Sentry, John W.

doi:10.3233/hab-2006-15302

Cited by 6 publications

(4 citation statements)

References 39 publications

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“…The clinical relevance of the five mitotic patterns is summarised in table 3,102–122 with more detailed information in online supplementary table S3. As for the cytoplasmic patterns, clinical associations for the mitotic patterns are primarily based on antigen-specific immunoassays and not on the HEp-2 IIFA pattern as such.…”

Section: Resultsmentioning

confidence: 99%

Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective

Damoiseaux

Andrade

Carballo³

et al. 2019

Ann Rheum Dis

254

304

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The indirect immunofluorescence assay (IIFA) on HEp-2 cells is widely used for detection of antinuclear antibodies (ANA). The dichotomous outcome, negative or positive, is integrated in diagnostic and classification criteria for several systemic autoimmune diseases. However, the HEp-2 IIFA test has much more to offer: besides the titre or fluorescence intensity, it also provides fluorescence pattern(s). The latter include the nucleus and the cytoplasm of interphase cells as well as patterns associated with mitotic cells. The International Consensus on ANA Patterns (ICAP) initiative has previously reached consensus on the nomenclature and definitions of HEp-2 IIFA patterns. In the current paper, the ICAP consensus is presented on the clinical relevance of the 29 distinct HEp-2 IIFA patterns. This clinical relevance is primarily defined within the context of the suspected disease and includes recommendations for follow-up testing. The discussion includes how this information may benefit the clinicians in daily practice and how the knowledge can be used to further improve diagnostic and classification criteria.

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Section: Resultsmentioning

confidence: 99%

Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective

Damoiseaux

Andrade

Carballo³

et al. 2019

Ann Rheum Dis

254

304

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“…3A). Double immunohistochemistry using AUF1 antibody and an antibody raised against PH3, a marker of proliferating cells in the M phase of cell cycle (Brenner et al 2003;Rayzman and Sentry 2006 showed that virtually all PH3+ cells in the VZ also expressed AUF1 (Fig. 3B,C).…”

Section: Auf1 Is Expressed By Proliferating Precursors/ Progenitors and Postmitotic Differentiating Neurons In The Cerebral Cortexmentioning

confidence: 99%

Members of the NuRD Chromatin Remodeling Complex Interact with AUF1 in Developing Cortical Neurons

et al. 2008

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Chromatin remodeling plays an important role in coordinating gene expression during cortical development, however the identity of molecular complexes present in differentiating cortical neurons that mediate the process remains poorly understood. The A + U-rich element-binding factor 1 (AUF1) is a known regulator of messenger RNA stability and also acts as a transcription factor upon binding to AT-rich DNA elements. Here we show that AUF1 is specifically expressed in subsets of proliferating neural precursors and differentiating postmitotic neurons of the developing cerebral cortex. Moreover, AUF1 is coexpressed with histone deacetylase 1 (HDAC1) and metastasis-associated protein 2 (MTA2), members of the nucleosome remodeling and histone deacetylase complex. AUF1 specifically and simultaneously binds to HDAC1, MTA2, and AT-rich DNA element, its gene regulatory function is modulated by the extent of histone acetylation and in animals lacking AUF1, the composition of the complex is modified. These results suggest that AUF1 is involved in integrating genetic and epigenetic signals during cortical development through recruiting HDAC1 and MTA2 to AT-rich DNA elements.

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“…Although autoAbs to the non-modified H3 N-terminal tail have been reported [8], the existence of autoAbs to the modified H3 1–19 N-terminal tail is unknown. One study reported H3K9me3S10ph in an isolated case from a patient who had discoid lupus erythematosus and chronic lymphocytic leukemia [23].…”

Section: Introductionmentioning

confidence: 99%

Systemic Lupus Erythematosus Patients Contain Significantly Less IgM against Mono-Methylated Lysine than Healthy Subjects

Guo

Liu

et al. 2013

PLoS ONE

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Post-translational modifications on proteins are important in biological processes but may create neo-epitopes that induce autoimmune responses. In this study, we measured the serum IgG and IgM response to a set of non-modified or acetyl- and methyl-modified peptides corresponding to residues 1–19 of the histone 3 N-terminal tail in systemic lupus erythematosus (SLE) patients and healthy subjects. Our results indicated that the SLE patients and healthy subjects produced antibodies (Abs) to the peptides, but the two groups had different Ab isotype and epitope preferences. Abs to the non-modified form, H31–19, were of the IgG isotype and produced by SLE patients. They could not recognize the scrambled H31–19, which contained the same amino acid composition but a different sequence as H31–19. In comparison, healthy subjects in general did not produce IgG against H31–19. However, about 70% of the healthy subjects produced IgM Abs against mono-methylated K9 of H31–19 (H31–19K9me). Our further studies revealed that ε-amine mono-methylated lysine could completely inhibit the IgM binding to H31–19K9me, but lysine had no inhibitory effect. In addition, the IgM Abs could bind peptides containing a mono-methylated lysine residue but with totally different sequences. Thus, mono-methylated lysine was the sole epitope for the IgM. Interestingly, SLE patients had much lower levels of this type of IgM. There was no obvious correlation between the IgM levels and disease activity and the decreased IgM was unlikely caused by medical treatments.We also found that the IgM Abs were not polyreactive to dsDNA, ssDNA, lipopolysaccharide (LPS) or insulin and they did not exist in umbilical cord serum, implying that they were not natural Abs. The IgM Abs against mono-methylated lysine are present in healthy subjects but are significantly lower in SLE patients, suggesting a distinct origin of production and special physiological functions.

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MCA1 detection of Histone H3 serine 10 phosphorylation, a novel biomarker for determination of mitotic indices

Cited by 6 publications

References 39 publications

Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective

Clinical relevance of HEp-2 indirect immunofluorescent patterns: the International Consensus on ANA patterns (ICAP) perspective

Members of the NuRD Chromatin Remodeling Complex Interact with AUF1 in Developing Cortical Neurons

Systemic Lupus Erythematosus Patients Contain Significantly Less IgM against Mono-Methylated Lysine than Healthy Subjects

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