MC13-0080 Somatic mutations of the EGFR, KRAS and BRAF genes: Heterogeneity in circulating epithalial tumor cells (CETC) as determined using the Cobas® Z 480 analyzer

Pachmann, Katharina; Pizon, Monika; Zimon, Dorothea; Stein, Emily

doi:10.1016/s0959-8049(13)70185-0

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“…As shown in the materials and methods section, the approach for picking individual cells ensured that only the targeted cell was aspirated and deposited. Previous studies showed that cells isolated in this way carry the same mutations as their corresponding primary tumor and also express its tumor tissue characteristics [ 17 , 24 ].…”

Section: Resultsmentioning

confidence: 99%

Prospective Monitoring of Circulating Epithelial Tumor Cells (CETC) Reveals Changes in Gene Expression during Adjuvant Radiotherapy of Breast Cancer Patients

et al. 2021

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Circulating epithelial tumor cells (CETC) are considered to be responsible for the formation of metastases. Therefore, their importance as prognostic and/or predictive markers in breast cancer is being intensively investigated. Here, the reliability of single cell expression analyses in isolated and collected CETC from whole blood samples of patients with early-stage breast cancer before and after radiotherapy (RT) using the maintrac® method was investigated. Single-cell expression analyses were performed with qRT-PCR on a panel of selected genes: GAPDH, EpCAM, NANOG, Bcl-2, TLR 4, COX-2, PIK3CA, Her-2/neu, Vimentin, c-Met, Ki-67. In all patients, viable CETC were detected prior to and at the end of radiotherapy. In 7 of the 9 (77.8%) subjects examined, the CETC number at the end of the radiotherapy series was higher than before. The majority of genes analyzed showed increased expression after completion of radiotherapy compared to baseline. Procedures and methods used in this pilot study proved to be feasible. The method is suitable for further investigation of the underlying molecular biological mechanisms occurring in cells surviving radiotherapy and possibly the development of radiation resistance.

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Section: Resultsmentioning

confidence: 99%