MBW complexes impinge on anthocyanidin reductase gene regulation for proanthocyanidin biosynthesis in persimmon fruit

Abstract

MBW protein complexes containing MYB, bHLH and WD40 repeat factors are known transcriptional regulators of secondary metabolites production such as proanthocyanidins and anthocyanins, and developmental processes such as trichome formation in many plant species. DkMYB2 and DkMYB4 (MYB-type), DkMYC1 (bHLH-type) and DkWDR1 (WD40-type) factors have been proposed by different authors to take part of persimmon MBW complexes for proanthocyanidin accumulation in immature fruit, leading to its characteristic astringent flavour with important agronomical and ecological effects. We have confirmed the nuclear localization of these proteins and their mutual physical interaction by bimolecular fluorescence complementation analysis. In addition, transient expression of DkMYB2, DkMYB4 and DkMYC1 cooperatively increase the expression of a persimmon anthocyanidin reductase gene (ANR), involved in the biosynthesis of cis-flavan-3-ols, the structural units of proanthocyanidin compounds. collectively, these data support the presence of MBW complexes in persimmon fruit and suggest their coordinated participation in ANR regulation for proanthocyanidin production.

The impact of 1‐MCP treatment and controlled atmosphere storage on the postharvest performance of four ( Chaenomeles japonica (Thunb.) Lindl. ex Spach) fruit cultivars

“…The astringency, being a distinctive characteristic of Chaenomeles fruit taste, is present due to the high content of proanthocyanidins (TPCC). According to the literature, the amount of TPCC in fruit may vary depending on the maturity, agricultural practices as well species (Gil‐Muñoz et al., 2020; Yu et al., 2020). The research available so far indicates that the amount of TPCC in JQ fruit varies from 4.0 to 6.3 g/kg (Miao et al., 2016; Watychowicz et al., 2017).…”

Regulation of Plant Tannin Synthesis in Crop Species

“…Several transcription factors crucial for PA accumulation in persimmon have been identified as DkMYB2/4 , DkMYC1, and DkWDR1 as homologs of TT2 , TT8, and TTG1 , respectively ( Akagi et al, 2009b ; Akagi et al, 2010a ; Naval et al, 2016 ). In yeast and Nicotiana benthamiana, it has been shown that DkMYB2, Shalini, and DkWDR1 interact together to act as a MBW-complex ( Gil-Muñoz et al, 2020 ). Moreover, in PCNA fruits, Dk MYB4 regulates the expression of ANR and F3′5′H ( Akagi et al, 2009b ).…”

Recent Advanced Metabolic and Genetic Engineering of Phenylpropanoid Biosynthetic Pathways

“…It is the polymers of flavan-3-ols and the resultant product of flavonoid pathway [81]. PA is the most extensively spread secondary metabolites and is mainly prominent in forest trees and woody plants [82]. In trees, proanthocyanidins are general constituents of vegetative parts which consist of flowers, leaves, bark, seed and roots [83], and provide protection to plants from various abiotic and biotic stressors.…”