MBP-1 mediated apoptosis involves cytochrome c release from mitochondria

Ghosh, Asish K.; Majumder, Mainak; Steele, Robert; Liu, Ta Jën; Ray, Ratna B.

doi:10.1038/sj.onc.1205384

Cited by 22 publications

(27 citation statements)

References 51 publications

(47 reference statements)

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“…The results suggested that MBP-1 forms a complex with MEK5␣ in vivo. DISCUSSION We have previously shown that MBP-1 induces cell death (12,15). MEK5 overexpression in prostate cancer has been implicated with tumor metastases and unfavorable survival outcome (24).…”

Section: Mbp-1 Expression Inhibits Mek5/bmk1-mediated Activation Of Nmentioning

confidence: 99%

“…Physiologic cell death occurs primarily through an evolutionarily conserved form of cell suicide, termed apoptosis or programmed cell death, and can be influenced by a wide variety of regulatory stimuli (19 -22). Exogenous expression of MBP-1 induces apoptosis in a number of different cancer cells (15). Recently, we have shown that exogenous expression of MBP-1 in androgen-independent prostate cancer cells inhibits cell proliferation (23).…”

mentioning

confidence: 99%

“…MBP-1, ubiquitously expressed in different human tissues (9), is located at human chromosome 1p35-pter (10). MBP-1, an ϳ37-kDa cellular protein, has many intriguing properties such as transcriptional repressor activity, induction of apoptosis, and regression of tumor growth (8,(11)(12)(13)(14)(15). Sequence analysis suggests that MBP-1 has a high homology with Eno1 cDNA (8), designated as human ␣-enolase cDNA (16).…”

mentioning

confidence: 99%

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c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

Ghosh

Steele

Ray

2005

Journal of Biological Chemistry

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Prostate cancer is the most common and invasive type of cancer among American men, and the second leading cause of cancer-elated deaths in the United States. Unfortunately, an effective therapeutic regimen is still lacking for advance stages of the disease. Recently, MEK5 has been shown to overexpress in prostate cancer and is associated with poor survival outcome. MEK5 exists as ␣-and ␤-isoforms.

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Section: Mbp-1 Expression Inhibits Mek5/bmk1-mediated Activation Of Nmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

Ghosh

Steele

Ray

2005

Journal of Biological Chemistry

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“…We have shown that intratumor injection of MBP-1 inhibits prostate and lung tumor growth in xenograft nude mice and induces cell death in several cancer cells without affecting the normal cell growth (15)(16)(17). Ubiquitous antitumor activities were also observed with other growth suppressors.…”

Section: Introductionmentioning

confidence: 84%

“…Cells were cotransfected with MMP-2 or MMP-9 promoter with luciferase gene and increasing doses of cytomegalovirus MBP-1 plasmid DNAs. Luciferase activity was measured 48 h posttransfection as described previously (15).…”

Section: Methodsmentioning

confidence: 99%

MBP-1 Inhibits Breast Cancer Growth and Metastasis in Immunocompetent Mice

et al. 2009

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Breast cancer is the leading cause of cancer death among women. We have shown previously an antiproliferative effect of MBP-1 on several human cancer cells. In this study, we have examined the potential of MBP-1 as a gene therapeutic candidate in regression of breast cancer growth and metastasis in an immunocompetent mouse model. For this, we have used a mouse breast cancer cell line (EO771) and syngeneic C57BL/6 mice. EO771 cells were implanted into the mammary fat pad of C57BL/6 mice. Replication-deficient recombinant adenovirus expressing MBP-1 was administered intratumorally to determine gene therapeutic potential. The results showed a significant regression of primary and distant (lung) tumor growth. Animals exhibited prolonged survival on treatment with MBP-1 compared with the control group (dl312). Subsequent studies suggested that MBP-1 inhibits matrix metalloproteinase expression in human breast cancer cells. Cells transduced with MBP-1 displayed inhibition of migration in a wound-healing assay. The conditioned medium from MBP-1-transduced cells blocked in vitro tube formation assay and inhibited expression of several angiogenic molecules. Taken together, our study shows that MBP-1 acts as a double-edged sword by inhibiting primary and metastatic tumor growth and modulating matrix metalloproteinase expression with a therapeutic potential against breast cancer progression. [Cancer Res 2009;69(24):9354-9]

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Proteomic analysis of apoptosis related proteins regulated by proto‐oncogene protein DEK

Kim

Chae

Kim

et al. 2009

J of Cellular Biochemistry

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A nuclear phosphoprotein, DEK, is implicated in certain human diseases, such as leukemia and antoimmune disorders, and a major component of metazoan chromatin. Basically as a modulator of chromatin structure, it can involve in various DNA and RNA-dependent processes and function as either an activator or repressor. Despite of numerous efforts to suggest the biological role of DEK, direct target proteins of DEK in different physiological status remains elusive. To investigate if DEK protein triggers the changes in certain protein networks, DEK was knocked down at both types of cell clones using siRNA expression. Here we provide a catalogue of proteome profiles in total cell lysates derived from normal HeLa and DEK knock-down HeLa cells and a good in vitro model system for dissecting the protein networks due to this proto-oncogenic DEK protein. In this biological context, we compared total proteome changes by the combined methods of two-dimensional gel electrophoresis, quantitative image analysis and MALDI-TOF MS analysis. There were a large number of targets for DEK, which were differentially expressed in DEK knock-down cells and consisted of 58 proteins (41 up-regulated and 17 down-regulated) differentially regulated expression was further confirmed for some subsets of candidates by Western blot analysis using specific antibodies. In the identified 58 spots, 16% of proteins are known to be associated with apoptosis. Among others, we identified apoptosis related proteins such as Annexins, Enolase1, Lamin A, and Glutathione-S-transferase omega 1. These results are consistent with recent studies indicating the crucial role of DEK in apoptosis pathway. We further demonstrated by ChIP analysis that knock-down of DEK caused hyperacetylation of histones around Prx VI promoter which is upregulated in our profile. Using immunoblotting analysis, we have demonstrated the modulation of other caspase-dependent apoptosis related proteins by DEK knock-down and further implicate its role in apoptosis pathway.

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MBP-1 mediated apoptosis involves cytochrome c release from mitochondria

Cited by 22 publications

References 51 publications

c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

c-myc Promoter-binding Protein 1 (MBP-1) Regulates Prostate Cancer Cell Growth by Inhibiting MAPK Pathway

MBP-1 Inhibits Breast Cancer Growth and Metastasis in Immunocompetent Mice

Proteomic analysis of apoptosis related proteins regulated by proto‐oncogene protein DEK

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