mBAND analysis of chromosome aberrations in lymphocytes exposed<i>in vitro</i>to α-particles and γ-rays

Ej, Tawn; Ca, Whitehouse; Holdsworth, Duncan; Ruyck, Kim De; Vandenbulcke, Katia; Thierens, Hubert

doi:10.1080/09553000802078412

Cited by 17 publications

(6 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…In vitro studies on the induction of chromosome aberrations by a-particle radiation have revealed the complexity of the damage (Anderson et al 2000;Moquet et al 2001;Anderson et al 2002Anderson et al , 2003Barquinero et al 2004;Anderson et al 2007;Tawn et al 2007Tawn et al , 2008Curwen et al 2012). A densely ionizing a-particle will only intersect a small fraction of the cell volume and the deposition of energy and resulting DNA damage will be localized along the particle track, resulting in multiple rearrangements.…”

Section: Introductionmentioning

confidence: 97%

“…However, more detailed analyses revealed that most insertions and inversions were part of complex unstable aberrations, and therefore very few would be transmissible (Barquinero et al 2004;Tawn et al 2007Tawn et al , 2008Curwen et al 2012). Nevertheless, the application of multi-coloured FISH (mFISH) has shown that, whilst the majority of aparticle-irradiated cells will suffer complex non-transmissible chromosome damage, a significant proportion survive with simple exchanges, in particular translocations, that can be passed on to descendant cells (Anderson et al 2005;Curwen et al 2012).…”

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Sotnik

et al. 2015

Radiat Environ Biophys

Self Cite

View full text Add to dashboard Cite

mFISH analysis of chromosome aberration profiles of 47 and 144 h lymphocyte cultures following exposure to 193 mGy α-particle radiation confirmed that the frequency of stable aberrant cells and stable cells carrying translocations remains constant through repeated cell divisions. Age-specific rates and in vitro dose-response curves were used to derive expected translocation yields in nine workers from the Mayak nuclear facility in Russia. Five had external exposure to γ-radiation, two of whom also had exposure to neutrons, and four had external exposure to γ-radiation and internal exposure to α-particle radiation from incorporated plutonium. Doubts over the appropriateness of the dose response used to estimate translocations from the neutron component made interpretation difficult in two of the workers with external exposure, but the other three had translocation yields broadly in line with expectations. Three of the four plutonium workers had translocation yields in line with expectations, thus supporting the application of the recently derived in vitro α-particle dose response for translocations in stable cells. Overall this report demonstrates that with adequate reference in vitro dose-response curves, translocation yield has the potential to be a useful tool in the validation of red bone marrow doses resulting from mixed exposure to external and internal radiation.

show abstract

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 98%

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Sotnik

et al. 2015

Radiat Environ Biophys

Self Cite

View full text Add to dashboard Cite

show abstract

“…Hybridisation, post-hybridisation washes and signal detection conditions were carried out following the method described by Tawn et al . 5 …”

Section: Methodsmentioning

confidence: 99%

Breakpoint mapping by whole genome sequencing identifiesPTH2Rgene disruption in a patient with midline craniosynostosis and a de novo balanced chromosomal rearrangement

et al. 2015

View full text Add to dashboard Cite

BackgroundCraniosynostosis (CRS) is a premature closure of calvarial sutures caused by gene mutation or environmental factors or interaction between the two. Only a small proportion of non-syndromic CRS (NSC) patients have a known genetic cause, and thus, it would be meaningful to search for a causative gene disruption for the development NSC. We applied a whole genome sequencing approach on a 15-month-old boy with sagittal and metopic synostosis to identify a gene responsible for the development of the disease.Methods and resultsConventional chromosome study revealed a complex paracentric inversion involving 2q14.3 and 2q34. Array comparative genomic hybridisation did not show any copy number variation. Multicolour banding analysis was carried out and the breakpoints were refined to 2q14 and 2q34. An intronic break of the PTH2R gene was detected by whole genome sequencing and fluorescence in situ hybridisation analysis confirmed disruption of PTH2R.ConclusionsWe report PTH2R as a gene that is disrupted in NSC. The disruption of the PTH2R gene may cause uncontrolled proliferation and differentiation of chondrocytes, which in turn results in premature closure of sutures. This addition of PTH2R to the list of genes associated with NSC expands our understanding of the development of NSC.

show abstract

“…However, the ratio of complex to simple interchanges (C ratio) was found higher for high LET radiation as compared to X-rays and the authors suggested that the C ratio should therefore be considered for biophysical modeling studies. Tawn et al [129] demonstrated a much higher induction of intrachromosomal changes by α-particles relative to rays. Using sister chromatid differentiation technique, Cartwright et al [130] reported the dependence of both radiation quality and chromatin structure on inversion frequencies in CHO cells.…”

Section: Multicolor Band For Detection Of Intrachromosomal Changesmentioning

confidence: 97%

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Balajee¹

2018

obm genet

View full text Add to dashboard Cite

The technique of in situ hybridization (ISH) using radioactively labeled DNA probes was first described in the late 1960s and early 1970s. The first use of fluorescence in situ hybridization (FISH) was reported in 1980s where RNA labeled with a fluorophore at the 3' end was used to detect specific DNA sequences. Since then, the technique has undergone various modifications for detecting single genes, chromosomes and whole genomes on various targets such as interphase nucleus, prematurely condensed chromosomes, metaphase chromosomes, fresh and paraffinized tissue sections. Although FISH is quite frequently used in clinical diagnostics, its use has recently been extended to the field of radiation biodosimetry where both stable (translocations) and unstable aberrations (dicentrics and rings) are detected with high resolution for estimating the absorbed radiation dose in humans after incidental, accidental or occupational exposure to ionizing radiation. This review summarizes the diverse applications of FISH in radiation biodosimetry that range from radiation dose estimation to prediction of deterministic (i.e. acute radiation syndrome severity) and stochastic (i.e. genetic mutations and cancer) effects in the affected human population.

show abstract

mBAND analysis of chromosome aberrations in lymphocytes exposedin vitroto α-particles and γ-rays

Cited by 17 publications

References 34 publications

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Chromosome aberrations in workers with exposure to α-particle radiation from internal deposits of plutonium: expectations from in vitro studies and comparisons with workers with predominantly external γ-radiation exposure

Breakpoint mapping by whole genome sequencing identifiesPTH2Rgene disruption in a patient with midline craniosynostosis and a de novo balanced chromosomal rearrangement

Applications of Fluorescence in Situ Hybridization in Radiation Cytogenetic Biodosimetry and Population Monitoring

Contact Info

Product

Resources

About