MazG, a Nucleoside Triphosphate Pyrophosphohydrolase, Interacts with Era, an Essential GTPase in<i>Escherichia coli</i>

Zhang, Junjie; Inouye, Masayori

doi:10.1128/jb.184.19.5323-5329.2002

Cited by 73 publications

(77 citation statements)

References 32 publications

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“…Another protein involved in this system is MazG, which was originally identified in a yeast two-hybrid screen for proteins interacting with Era (an essential E. coli GTPase) (287). As its name implies, mazG is located immediately downstream from mazEF (287) and is cotranscribed with mazEF (98). While the inactivation of mazG alone did not affect the viability of E. coli under standard growth conditions (287), the ectopic overexpression of mazG in a mazEFG mutant severely inhibited cell growth (98).…”

Section: Toxin-antitoxin Systemsmentioning

confidence: 99%

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Molecular Control of Bacterial Death and Lysis

Rice

Bayles

2008

Microbiol Mol Biol Rev

319

312

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SUMMARY Although the phenomenon of bacterial cell death and lysis has been studied for over 100 years, the contribution of these important processes to bacterial physiology and development has only recently been recognized. Contemporary study of cell death and lysis in a number of different bacteria has revealed that these processes, once thought of as being passive and unregulated, are actually governed by highly complex regulatory systems. An emerging paradigm in this field suggests that, analogous to programmed cell death in eukaryotes, regulated cell death and lysis in bacteria play an important role in both developmental processes, such as competence and biofilm development, and the elimination of damaged cells, such as those irreversibly injured by environmental or antibiotic stress. Further study in this exciting field of bacterial research may provide new insight into the potential evolutionary link between control of cell death in bacteria and programmed cell death (apoptosis) in eukaryotes.

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Section: Toxin-antitoxin Systemsmentioning

confidence: 99%

Section: Toxin-antitoxin Systemsmentioning

confidence: 99%

Molecular Control of Bacterial Death and Lysis

Rice

Bayles

2008

Microbiol Mol Biol Rev

319

312

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“…2B) as described previously (6). The synthesis of MazG protein (30 kDa) (15) from plasmid pET-11a-MazG was tested at 37°C for 1 h in the absence and presence of YoeB using an E. coli T7 S30 extract system (Promega) (Fig. 2C).…”

Section: Volume 284 • Number 11 • March 13 2009mentioning

confidence: 99%

The Inhibitory Mechanism of Protein Synthesis by YoeB, an Escherichia coli Toxin

Zhang

Inouye

2009

Journal of Biological Chemistry

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101

163

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YoeB is a toxin encoded by the yefM-yoeB antitoxin-toxin operon in the Escherichia coli genome. Here we show that YoeB, a highly potent protein synthesis inhibitor, specifically blocks translation initiation. In in vivo primer extension experiments using two different mRNAs, a major band was detected after YoeB induction at three bases downstream of the initiation codon at 2.5 min. An identical band was also detected in in vitro toeprinting experiments after the addition of YoeB to the reaction mixtures containing 70 S ribosomes and the same mRNAs, even in the absence of tRNA f Met . Notably, this band was not detected in the presence of YoeB alone, indicating that YoeB by itself does not have endoribonuclease activity under the conditions used. The 70 S ribosomes increased upon YoeB induction, and YoeB was found to be specifically associated with 50 S subunits. Using tetracycline and hygromycin B, we demonstrated that YoeB binds to the 50 S ribosomal subunit in 70 S ribosomes and interacts with the A site leading to mRNA cleavage at this site. As a result, the 3-end portion of the mRNA was released from ribosomes, and translation initiation was effectively inhibited. These results demonstrate that YoeB primarily inhibits translation initiation.

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“…These are highly conserved proteins in bacteria and to date there has been no tangible link demonstrated between MazG and salt tolerance. MazG is a nucleoside triphosphate pyrophosphohydrolase (NTPase), which can hydrolyse (deoxy)ribonucleoside triphosphates ((d)NTPs) to their corresponding (deoxy)ribonucleoside monophosphates ((d)NMPs) and pyrophosphate (PPi) (Zhang and Inouye, 2002). It has been proposed that MazG has a role in cellular 'house-cleaning' by removing abnormal (d)NTP's from nascent DNA strands (Galperin et al, 2006), in addition to regulating programmed cell death in E. coli (Gross et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome

et al. 2012

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Metagenomics is a powerful tool that allows for the culture-independent analysis of complex microbial communities. One of the most complex and dense microbial ecosystems known is that of the human distal colon, with cell densities reaching up to 10 12 per gram of faeces. With the majority of species as yet uncultured, there are an enormous number of novel genes awaiting discovery. In the current study, we conducted a functional screen of a metagenomic library of the human gut microbiota for potential salt-tolerant clones. Using transposon mutagenesis, three genes were identified from a single clone exhibiting high levels of identity to a species from the genus Collinsella (closest relative being Collinsella aerofaciens) (COLAER_01955, COLAER_01957 and COLAER_01981), a high G þ C, Gram-positive member of the Actinobacteria commonly found in the human gut. The encoded proteins exhibit a strong similarity to GalE, MurB and MazG. Furthermore, pyrosequencing and bioinformatic analysis of two additional fosmid clones revealed the presence of an additional galE and mazG gene, with the highest level of genetic identity to Akkermansia muciniphila and Eggerthella sp. YY7918, respectively. Cloning and heterologous expression of the genes in the osmosensitive strain, Escherichia coli MKH13, resulted in increased salt tolerance of the transformed cells. It is hoped that the identification of atypical salt tolerance genes will help to further elucidate novel salt tolerance mechanisms, and will assist our increased understanding how resident bacteria cope with the osmolarity of the gastrointestinal tract.

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MazG, a Nucleoside Triphosphate Pyrophosphohydrolase, Interacts with Era, an Essential GTPase inEscherichia coli

Cited by 73 publications

References 32 publications

Molecular Control of Bacterial Death and Lysis

Molecular Control of Bacterial Death and Lysis

The Inhibitory Mechanism of Protein Synthesis by YoeB, an Escherichia coli Toxin

Functional metagenomics reveals novel salt tolerance loci from the human gut microbiome

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