Maximizing the enzyme immobilization of enzymatic glucose biofuel cells through hierarchically structured reduced graphene oxide

Lee, Joonyoung; Hyun, Kyuhwan; Park, Jaemin; Park, Ho Seok; Kwon, Yongchai

doi:10.1002/er.7155

Cited by 19 publications

(8 citation statements)

References 77 publications

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“…Electrochemical mediators have been extensively used to facilitate the low potential bioelectrocatalytic transformation for glucose by GOx in biosensing applications . TTF was found to produce the lowest oxidation potential and exhibit the most stable performance .…”

Section: Resultsmentioning

confidence: 99%

“…Electrochemical mediators have been extensively used to facilitate the low potential bioelectrocatalytic transformation for glucose by GOx in biosensing applications. 61 TTF was found to produce the lowest oxidation potential and exhibit the most stable performance. 53 β-D-Glucose was diffused from the solution to the electrode surface and oxidized by GOx (FAD) and GOx (FADH 2 ) was resumed to GOx (FAD) by TTF + .…”

Section: Characterization Of Cnt-aunp-gox Bioanodementioning

confidence: 99%

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Carbon Nanotube-Gold Nanoparticle-Based Self-Powered Electrochemical Biosensors for Highly Sensitive and Stable Detection of Myoglobin

Zhu

Sun

Yin

et al. 2023

ACS Appl. Nano Mater.

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A convenient and ultrasensitive self-powered aptasensing device based on an enzymatic biofuel cell (EBFC) was developed for myoglobin (Mb) detection. The self-powered aptasensor was composed of a functionalized biocathode with carbon nanotube-gold nanoparticle-aptamer (CNT-AuNP-aptamer) and a bioanode with carbon nanotube-gold nanoparticleglucose oxidase (CNT-AuNP-GOx). To ensure the performance of the self-powered aptasensor, the CNT-AuNP hybrid was selected as the electrode material because of its excellent biocompatibility, superior electrical conductivity, and chemical stability. Meanwhile, aptamer was assembled onto the CNT-AuNP functionalized biocathode through the Au−S bond. When the Mb was present, the aptamer could capture it on the biocathode via specific recognition, and the significant steric hindrance efficiently blocked the electronic transport for the redox probe [Fe(CN) 6 ] 3− at the biocathode, which caused a dramatic decrease of the open-circuit voltage (E OCV ). Promisingly, this self-powered aptasensor has particularly high sensitivity for Mb detection over a wide concentration spectrum of 0.1−1 × 10 4 ng mL −1 ; the detection limit is as low as 0.011 ng mL −1 (S/N = 3), and the sensor has excellent selectivity, reproducibility, and stability. In addition, the results of the relative standard deviation (RSD) (3.19−5.34%) and recovery (99.27−101.34%) indicated that the aptasensor can be applied to accurately assay Mb within complex biological matrices. As in situ Mb monitoring is of crucial clinical diagnostic significance, this work not only provides an intelligent method for ultrasensitive biosensing of Mb but also is a prospective prototype for a portable and in situ biomedical sensor.

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Section: Resultsmentioning

confidence: 99%

Section: Characterization Of Cnt-aunp-gox Bioanodementioning

confidence: 99%

Carbon Nanotube-Gold Nanoparticle-Based Self-Powered Electrochemical Biosensors for Highly Sensitive and Stable Detection of Myoglobin

Zhu

Sun

Yin

et al. 2023

ACS Appl. Nano Mater.

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“…[28,29] To increase such a low electron transfer rate, a mediator was considered, [30] and TTF was used for this purpose in the present work. [31,32] TTF is a kind of heterocyclic organic compounds and has excellent conductivity with a redox potential of 0.05 V, which is the proper potential for transfer of electrons between mediator and FAD cofactor having redox potential of −0.46 V. In addition, due to its unique heterocyclic chemical structure, TTF interacts with GOx by 𝜋−𝜋 interaction, enhancing adhesion between them. [33] As shown in Figure 3a, the onset potential of GOR observed in flat Au anode with PTFE substrate was 0.05 V, with current density of 0.37 mA cm −2 at 0.3 V. These were 0.05 V and 0.31 mA cm −2 at 0.3 V in non-buckled flat Au anode on PDMS substrate (Figure 3c).…”

Section: Electrochemical Characterizations Of Different Anode and Cat...mentioning

confidence: 99%

Stretchable Enzymatic Biofuel Cells Based on Microfluidic Structured Elastomeric Polydimethylsiloxane with Wrinkled Gold Electrodes

Lee,

Kim,

Kwon

et al. 2023

Adv Funct Materials

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Various sensors and electronic devices are recently developed to monitor human health in mechanically flexible or even stretchable forms for intimate contact with non‐flat curvilinear surfaces of the human body. For successful operation of these devices, finding a proper way to electrically power them is very important. In this work, glucose/oxygen fueled enzymatic biofuel cells (EBFCs) based on microfluidic structured elastomeric polydimethylsiloxane substrate with wrinkled gold (Au) electrodes are suggested for power supply. For doing that, firstly, bottom surface of microfluidic channel is covered with buckled Au electrodes for stretchability. By microfluidic design showing capillary imbibition through fluidic channels, loading of catalyts is promoted. Interestingly, buckled Au electrodes induce much better anodic and cathodic reaction rates than those of non‐buckled Au electrodes by 25% and 33%, respectively. This is because surface area and the amount of catalyst loading in electrodes increase by Au wrinkling. In evaluations of EBFCs using the buckled Au electrodes, maximum power density reaches 7.1 ± 0.64 µW cm−2, while they show decent performance of 5.4 ± 0.49 µW cm−2 even under external stretching. Taken together, it is corroborated that such proposed stretchable EBFCs are alternative for providing electrical power in wearable or implantable devices.

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“…Kwon and Park et al have made modifications to the reduced graphene oxide (rGO) structure to increase enzyme immobilization sites, leading to improved performance of EBFCs [28]. They used rGO with three-dimensional porous structure, prepared by spray freeze-drying method, as the substrate material for immobilized GOx.…”

Section: Some Nanomaterials and Nanocomposites For Enzyme Immobilizat...mentioning

confidence: 99%

Research Progresses and Application of Biofuel Cells Based on Immobilized Enzymes

Zhou

Liu

et al. 2023

Applied Sciences

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Enzymatic biofuel cells (EBFCs) are devices that use natural enzymes as catalysts to convert chemical energy from bio-sourced fuels into electrical energy. In this review, we summarize recent research progress and applications in the field of biofuel cells based on immobilized enzymes. Specifically, we discuss how to optimize and improve the electrochemical performance and operational stability of enzymatic biofuel cells through enzyme immobilization materials, enzyme immobilization methods, electron transfer improvement on enzyme electrodes, and cell construction methods. We also cover current and future practical applications of biofuel cells based on immobilized enzymes, including implantable enzymatic biofuel cells and wearable enzymatic biofuel cells. Additionally, we present some of the issues that still need to be addressed in the field of biofuel cells based on immobilized enzymes to ensure their technical and commercial viability and sustainability.

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Maximizing the enzyme immobilization of enzymatic glucose biofuel cells through hierarchically structured reduced graphene oxide

Cited by 19 publications

References 77 publications

Carbon Nanotube-Gold Nanoparticle-Based Self-Powered Electrochemical Biosensors for Highly Sensitive and Stable Detection of Myoglobin

Carbon Nanotube-Gold Nanoparticle-Based Self-Powered Electrochemical Biosensors for Highly Sensitive and Stable Detection of Myoglobin

Stretchable Enzymatic Biofuel Cells Based on Microfluidic Structured Elastomeric Polydimethylsiloxane with Wrinkled Gold Electrodes

Research Progresses and Application of Biofuel Cells Based on Immobilized Enzymes

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