Maximizing fibroblast adhesion on protein-coated surfaces using microfluidic cell printing

Davidoff, Sherry N.; Au, D.; Gale, Bruce K.; Brooks, Benjamin D.; Brooks, Amanda E.

doi:10.1039/c5ra18673k

Cited by 4 publications

(2 citation statements)

References 50 publications

(97 reference statements)

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“…Therefore, if the cell culture can survive the highest concentration, then the cell growth density would even increase with decreasing SWCNT concentration in the composite. The fluorescence images ( Figure 5 a−h) show exemplary images of living fibroblasts cultured directly on the SWCNT-based nanocomposites within 24 and 48 h. For the 24 h reference ( Figure 5 d) samples, cell densities of ~90 mm −2 are obtained, which is comparable to standard values for fibroblast-coated substrates [ 57 ]. For 24 h albumin and acrylic polymer-based samples ( Figure 5 a,c), the density of cells increases strongly (~210 mm −2 ) in comparison to the reference.…”

Section: Resultsmentioning

confidence: 54%

Biocompatible SWCNT Conductive Composites for Biomedical Applications

Markov

Wördenweber²,

Ичкитидзе

et al. 2020

Nanomaterials

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The efficiency of devices for biomedical applications, including tissue engineering and neuronal stimulation, heavily depends on their biocompatibility and performance level. Therefore, it is important to find adequate materials that meet the necessary requirements such as (i) being intrinsically compatible with biological systems, (ii) providing a sufficient electronic conductivity that promotes efficient signal transduction, (iii) having “soft” mechanical properties comparable to biological structures, and (iv) being degradable in physiological solution. We have developed organic conducting biocompatible single-walled carbon nanotubes (SWCNT) composites based on bovine serum albumin, carboxymethylcellulose, and acrylic polymer and investigated their properties, which are relevant for biomedical applications. This includes ζ-potential measurements, conductivity analyses, and SEM micrographs, the latter providing a local analysis of SWCNT distribution in the base material. We observed the development of the electrical conductivity of the SWCNT composites exposed to 1 mM KCl electrolyte for 40 days, representing a high stability of the samples. The conductivity of samples reaches 1300 S/m for 0.45 wt.% nanotubes. Moreover, we demonstrated the biocompatibility of the composites via cultivating fibroblast cell culture. Finally, we showed that composite coating results in the longer lifespan of cells on the surface. Overall, the SWCNT-based conductive composites might be a promising material for extended biomedical applications.

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Section: Resultsmentioning

confidence: 54%

Biocompatible SWCNT Conductive Composites for Biomedical Applications

Markov

Wördenweber²,

Ичкитидзе

et al. 2020

Nanomaterials

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show abstract

“…Hence, they hold great applications in drug discovery chips, cell staining devices [9] and microbioreactors [10]. For cell immobilization, there are several approaches such as encapsulation within photo cross-linkable polymers [11], adhesion to patterned proteins [12] and protein coatings [13].…”

Section: Introductionmentioning

confidence: 99%

Novel criteria for the optimum design of grooved microchannels based on cell shear protection and docking regulation: a lattice Boltzmann method study

2020

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Grooved channel bioreactors have shown great applications in cell biology studies by creating a controlled cellular microenvironment and protecting it from destructive influences of fluidic shear stress. Despite numerous studies on improvement in cell docking and retention in microchannels, the lack of reliable criteria for determining optimal groove geometries seems to be a great barrier in the field. In this study, a systematic approach was used to find the critical geometrical parameters that yield to the highest cell shear protection against the upstream flow. To achieve this goal, the lattice Boltzmann method was used to simulate the flow inside a grooved microchannel due to its incredible reliability for portraying complex streamlines in microflow phenomenon. The simulation results showed that the flow behavior within microgrooves considerably varies with groove/channel geometry and that based on the generated microcirculation regions, there are correlations between groove/channel width, depth and the maximum shear protection factor, which led toward finding reliable criteria for optimization of such parameters. The results could be beneficial for researchers to design such devices based on different cell sizes, cell behavior and geometrical constraints while ensuring protected cell culture environment.

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Microfluidic Flow Cell Array for Controlled Cell Deposition in Engineered Musculoskeletal Tissues

Ede

Davidoff

Blitch

et al. 2018

Tissue Engineering Part C: Methods

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Musculoskeletal tissues contain critical gradients in extracellular matrix (ECM) composition and cell types that allow for proper mechanical function of tissues and integration between adjacent tissues. However, properly controlling these patterns in engineered tissues is difficult and tissue engineering (TE) is presently in need of methods to generate integration zones for tissue anchoring, transition zones between tissues, and controlled ECM gradients for proper mechanical function. In this study, we present a novel method of using a microfluidic flow cell array (MFCA) to precisely control cell deposition onto TE constructs to produce tunable cell patterns on engineered constructs. In this study, we characterized MFCA cell deposition to efficiently and reliably deposit cells in controllable patterns and densities. We developed methods for deposition of human adipose-derived stem cells and human osteoblasts using a 12-channel pilot printhead. We mimicked key gradients and transitions by creating two-cell and three-cell-type transitions characteristic of the integration zones of musculoskeletal tissues. Overall, we demonstrate the ability to precisely and reproducibly control cell deposition on engineered constructs using this method and control cell population gradients. We establish the production of multicell transitions and multicell interfaces utilizing MFCA cell deposition, to demonstrate the potential of the method to create an extensive variety of engineered musculoskeletal tissues. Furthermore, customization of the printhead design can accommodate various structures, sizes, shapes, and number of flow cell channels to meet specific requirements for a broad range of musculoskeletal tissues.

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Maximizing fibroblast adhesion on protein-coated surfaces using microfluidic cell printing

Cited by 4 publications

References 50 publications

Biocompatible SWCNT Conductive Composites for Biomedical Applications

Biocompatible SWCNT Conductive Composites for Biomedical Applications

Novel criteria for the optimum design of grooved microchannels based on cell shear protection and docking regulation: a lattice Boltzmann method study

Microfluidic Flow Cell Array for Controlled Cell Deposition in Engineered Musculoskeletal Tissues

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