Maximal locomotor depression follows maximal ankle swelling during the progression of arthritis in K/BxN mice

Frommholz, David; Illges, Harald

doi:10.1007/s00296-011-2337-5

Cited by 6 publications

(6 citation statements)

References 16 publications

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“…Rajasekaran et al[ 66 ] observed maximum decline in locomotor activity during the period when peak joint thickness occurred in the arthritic mice. Similar observation was made by Frommholz and Illges [ 67 ] who also showed an indirect correlation of ankle thickness and locomotor activity in arthritic K/BxN mice. Our study also demonstrates similar correlation between oedema scoring of infected limb and locomotion counts.…”

Section: Discussionsupporting

confidence: 89%

In Vivo Assessment of Phage and Linezolid Based Implant Coatings for Treatment of Methicillin Resistant S. aureus (MRSA) Mediated Orthopaedic Device Related Infections

2016

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Staphylococcus comprises up to two-thirds of all pathogens in orthopaedic implant infections with two species respectively Staphylococcus aureus and Staphylococcus epidermidis, being the predominate etiological agents isolated. Further, with the emergence of methicillin-resistant S. aureus (MRSA), treatment of S. aureus implant infections has become more difficult, thus representing a devastating complication. Use of local delivery system consisting of S.aureus specific phage along with linezolid (incorporated in biopolymer) allowing gradual release of the two agents at the implant site represents a new, still unexplored treatment option (against orthopaedic implant infections) that has been studied in an animal model of prosthetic joint infection. Naked wire, hydroxypropyl methylcellulose (HPMC) coated wire and phage and /or linezolid coated K-wire were surgically implanted into the intra-medullary canal of mouse femur bone of respective groups followed by inoculation of S.aureus ATCC 43300(MRSA). Mice implanted with K-wire coated with both the agents i.e phage as well as linezolid (dual coated wires) showed maximum reduction in bacterial adherence, associated inflammation of the joint as well as faster resumption of locomotion and motor function of the limb. Also, all the coating treatments showed no emergence of resistant mutants. Use of dual coated implants incorporating lytic phage (capable of self-multiplication) as well as linezolid presents an attractive and aggressive early approach in preventing as well as treating implant associated infections caused by methicillin resistant S. aureus strains as assessed in a murine model of experimental joint infection.

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Section: Discussionsupporting

confidence: 89%

In Vivo Assessment of Phage and Linezolid Based Implant Coatings for Treatment of Methicillin Resistant S. aureus (MRSA) Mediated Orthopaedic Device Related Infections

2016

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“…Inflammation and suppression of pain-related spontaneous behaviors, such as locomotor activity, operant responses, and place avoidance, are strongly correlated in rapid-onset models of arthritis [134]. However, the more slowly progressing K/BxN mouse strain shows a significant delay between peak clinical progression and decreased mobility [178]. This finding is suggestive of a psychoaffective component to pain-suppressed behaviors, which is commonly observed in human RA.…”

Section: Understanding the Molecular Mechanism Underlying The Pathogementioning

confidence: 94%

Understanding the Molecular Mechanisms Underlying the Pathogenesis of Arthritis Pain Using Animal Models

Hong

Park

Kim

2020

IJMS

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Arthritis, including osteoarthritis (OA) and rheumatoid arthritis (RA), is the leading cause of years lived with disability (YLD) worldwide. Although pain is the cardinal symptom of arthritis, which is directly related to function and quality of life, the elucidation of the mechanism underlying the pathogenesis of pain in arthritis has lagged behind other areas, such as inflammation control and regulation of autoimmunity. The lack of therapeutics for optimal pain management is partially responsible for the current epidemic of opioid and narcotic abuse. Recent advances in animal experimentation and molecular biology have led to significant progress in our understanding of arthritis pain. Despite the inherent problems in the extrapolation of data gained from animal pain studies to arthritis in human patients, the critical assessment of molecular mediators and translational studies would help to define the relevance of novel therapeutic targets for the treatment of arthritis pain. This review discusses biological and molecular mechanisms underlying the pathogenesis of arthritis pain determined in animal models of OA and RA, along with the methodologies used.

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“…The appraisal of horizontal locomotion was evaluated by the method of Frommholz et al The mice underwent an adapting phase of 5 minutes and were then recorded for 10 minutes. The actual motion tracking was done with SwisTrack (École Polytechnique Fédérale de Lausanne, Switzerland).…”

Section: Methodsmentioning

confidence: 99%

Comparative study on serum‐induced arthritis in the temporomandibular and limb joint of mice

et al. 2019

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Introduction:The subject of the present study was a systematic comparative analysis of the rheumatoid arthritis (RA)-induced pathomechanisms in the temporomandibular joint with those of the limb joints using the serum-induced arthritis K/BxN model. Methods:In 18 BALB/c mice the induction of RA was performed according to the Kouskoff method. Another healthy cohort served as controls (n = 12). Joint swelling of the paws was measured using a micrometer. Functional data were obtained analyzing locomotion. Three-dimensional examination of the temporomandibular joint was performed with micro-computed tomography imaging, followed by histological evaluation of the extremity joints and the temporomandibular joint. Additionally, immunohistochemical investigations were carried out to evaluate inflammatory and immunological changes.Results: Measurement of joint swelling showed a significant increase in the diameter of the paws, as well as a decrease in locomotor activity compared to control animals and the time before arthritis induction. Histological and immunohistochemical investigations showed clear signs of inflammation in the extremity joints. In contrast, no histological or immunohistochemical indications of an inflammatory process were detectable in the temporomandibular joint. In addition, the three-dimensional analysis by micro-computed tomography of the temporomandibular joints did not show any obvious morphological changes. Conclusion:For the first time, using the K/BxN model we could demonstrate that, due to its anatomical and mechanical conditions, the temporomandibular joint seems to be less susceptible to the initiation of RA compared to limb joints. Therefore, additional investigations are needed on other arthritis models as well, in order to further improve our understanding of the pathogenesis and defense mechanisms of the disease. K E Y W O R D SµCT, arthritis, immunhistochemistry, K/BxN, rheumatoid arthritis, temporomandibular joint

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Maximal locomotor depression follows maximal ankle swelling during the progression of arthritis in K/BxN mice

Cited by 6 publications

References 16 publications

In Vivo Assessment of Phage and Linezolid Based Implant Coatings for Treatment of Methicillin Resistant S. aureus (MRSA) Mediated Orthopaedic Device Related Infections

In Vivo Assessment of Phage and Linezolid Based Implant Coatings for Treatment of Methicillin Resistant S. aureus (MRSA) Mediated Orthopaedic Device Related Infections

Understanding the Molecular Mechanisms Underlying the Pathogenesis of Arthritis Pain Using Animal Models

Comparative study on serum‐induced arthritis in the temporomandibular and limb joint of mice

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