Maximal adherence and invasion of INT 407 cells by Campylobacter jejuni requires the CadF outer-membrane protein and microfilament reorganization

Campylobacter jejuni adhere to and invade chicken intestinal epithelial cells in vitro

“…In the present study, C. jejuni 81-176 was inhibited from invading primary cells previously exposed to inhibitors of actin accumulation. These results are in keeping with those recently reported for C. jejuni invasion of INT 407 cells by Monteville et al (2003). The contribution to cell entry of a variety of different cell processes, including lipid rafts and cytoskeleton components, is an emerging paradigm among enteric pathogens (Cossart & Sansonetti, 2004).…”

PCR Detection of Four Virulence-Associated Genes of Campylobacter jejuni Isolates from Thai Broilers and Their Abilities of Adhesion to and Invasion of INT-407 Cells

“…This result may be caused by geographic and species differences. The previous studies revealed that dnaJ, cadF, pldA and ciaB genes are important to host cell invasion and caecal colonization [20,31,34,43,44]. However, we could not find a clear association between the invasion ability and the presence of virulence-associated genes in this experiment, suggesting that more genes may be involved in the invasion process.…”

“…Four mutant strains derived from F38011, an excellent colonizer of chicken caeca, dnaJ, cadF, pldA and ciaB are incapable of colonizing the ceca [20,23,43,44]. cadF (Campylobacter adhesin to fibronectin) is an outer membrane protein, which encodes a protein that interacts with a host extracellular matrix protein fibronectin [31], and is required for Campylobacter adherence to and colonization of the host cell surface [20]. Other genes such as ciaB (Campylobacter invasive antigen B) [21,34] and pldA encoded outer membrane phospholipase A [34] are involved in host cell invasion and are important for caecal colonization [21,43].…”

Molecular mechanisms and biological role ofCampylobacter jejuniattachment to host cells

“…Binding of bacteria to Fn is required for host cell invasion and colonisation [11,16,22]. For example, cadF mutants had a 50% reduced ability to adhere to human INT 407 cells compared to the wild type [16].…”

“…Host cell receptors have been identified only for outer membrane proteins CadF and FlpA specific to fibronectin (Fn) [16][17][18][19] and JlpA, specific to heat shock protein 90 [20].…”