MAVS induces a host cell defense to inhibit CSFV infection

Wang, Dong; Lv, Huifang; Li, Cheng; Liu, Yaru; Wang, Chengbao; Lin, Jihui; Wang, Yifan; Gui, Qian; Guo, Kangkang; Zhang, Yanming

doi:10.1007/s00705-018-3804-z

Cited by 10 publications

(8 citation statements)

References 48 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…CSFV infection results in highly significant economic losses in the swine industry [37,38]. With the rampant prevalence of CSF, increasing attention has been paid to the prevention and control of CSF.…”

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Pang

et al. 2019

Cells

View full text Add to dashboard Cite

Successful RNAi applications depend on strategies allowing stable and persistent expression of minimal gene silencing triggers without perturbing endogenous gene expression. In this study, we proposed an endogenous microRNA (miRNA) cluster as a novel integration site for small hairpin RNAs (shRNAs). We successfully integrated exogenous shRNAs at the porcine miRNA-17-92 (pmiR-17-92) cluster via a CRISPR/Cas9-mediated knock-in strategy. The anti-EGFP or anti-CSFV shRNAs could be stably and effectively expressed at the control of the endogenous promoter of the pmiR-17-92 cluster. Importantly, we confirmed that hitchhike expression of anti- classical swine fever (CSFV) shRNA had no effect on cell growth, blastocyst development and endogenous pmiR-17-92 expression in selected transgene (TG) porcine fetal fibroblasts (PFFs) clones. Moreover, these TG PFFs could inhibit the replication of CSFV by half and could be further used for generation of transgenic pigs. Taken together, these results show that our RNA interference (RNAi) expression strategy benefits numerous applications, from miRNA, genome and transgenic research, to gene therapy.

show abstract

Section: Discussionmentioning

confidence: 99%

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Pang

et al. 2019

Cells

View full text Add to dashboard Cite

show abstract

“…There may be other reasons as well. It is well known that most acute infection deaths are caused by storms of inflammatory factors [33][34][35][36][37]. Inflammatory cytokine storms can cause a variety of diseases, including infection, acute respiratory distress syndrome, and sepsis.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers

Sun

Zhang

Fan

et al. 2021

Viruses

View full text Add to dashboard Cite

African swine fever virus (ASFV) causes acute hemorrhagic fever in domestic pigs and wild boars, resulting in incalculable economic losses to the pig industry. As the mechanism of viral infection is not clear, protective antigens have not been discovered or identified. In this study, we determined that the p30, pp62, p72, and CD2v proteins were all involved in the T cell immune response of live pigs infected with ASFV, among which p72 and pp62 proteins were the strongest. Panoramic scanning was performed on T cell epitopes of the p72 protein, and three high-frequency positive epitopes were selected to construct a swine leukocyte antigen (SLA)-tetramer, and ASFV-specific T cells were detected. Subsequently, the specific T cell and humoral immune responses of ASFV-infected pigs and surviving pigs were compared. The results demonstrate that the specific T cellular immunity responses gradually increased during the infection and were higher than that in the surviving pigs in the late stages of infection. The same trend was observed in specific humoral immune responses, which were highest in surviving pigs. In general, our study provides key information for the exploration of ASFV-specific immune responses and the development of an ASFV vaccine.

show abstract

“…(2) Some proteins can inhibit CSFV replication by regulating the NF-κB signaling pathway, such as TRAF6 [47], Trx2 [48], and Hsp27 [49]. (3) Some proteins can inhibit virus replication by affecting the activity of the RIG-I-like signaling pathway, such as HB [50] and MAVS [51]. (4) Proteins involved in the ubiquitin-proteasome system, such as pRNF114 [52] and PSMB10 [53], could also inhibit CSFV replication.…”

Section: Host Proteins With Anti-csfv Effectsmentioning

confidence: 99%

Anti-Classical Swine Fever Virus Strategies

et al. 2021

View full text Add to dashboard Cite

Classical swine fever (CSF), caused by CSF virus (CSFV), is a highly contagious swine disease with high morbidity and mortality, which has caused significant economic losses to the pig industry worldwide. Biosecurity measures and vaccination are the main methods for prevention and control of CSF since no specific drug is available for the effective treatment of CSF. Although a series of biosecurity and vaccination strategies have been developed to curb the outbreak events, it is still difficult to eliminate CSF in CSF-endemic and re-emerging areas. Thus, in addition to implementing enhanced biosecurity measures and exploring more effective CSF vaccines, other strategies are also needed for effectively controlling CSF. Currently, more and more research about anti-CSFV strategies was carried out by scientists, because of the great prospects and value of anti-CSFV strategies in the prevention and control of CSF. Additionally, studies on anti-CSFV strategies could be used as a reference for other viruses in the Flaviviridae family, such as hepatitis C virus, dengue virus, and Zika virus. In this review, we aim to summarize the research on anti-CSFV strategies. In detail, host proteins affecting CSFV replication, drug candidates with anti-CSFV effects, and RNA interference (RNAi) targeting CSFV viral genes were mentioned and the possible mechanisms related to anti-CSFV effects were also summarized.

show abstract

MAVS induces a host cell defense to inhibit CSFV infection

Cited by 10 publications

References 48 publications

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

CRISPR/Cas9-Mediated Hitchhike Expression of Functional shRNAs at the Porcine miR-17-92 Cluster

Evaluation of Cellular Immunity with ASFV Infection by Swine Leukocyte Antigen (SLA)—Peptide Tetramers

Anti-Classical Swine Fever Virus Strategies

Contact Info

Product

Resources

About