Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology

Arias, Soledad; Moral, Ana; Ferrer, M.R.; Tallon, Richard; Quesada, Emilia; Béjar, Victoria

doi:10.1007/s00792-003-0325-8

Cited by 178 publications

(152 citation statements)

References 23 publications

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“…They are widely distributed throughout hypersaline environments. Some of them are recognized for their potential use in biotechnology (Margesin & Schinner, 2001;Ventosa & Nieto, 1995), producing exopolysaccharides (Arias et al, 2003;Mata et al, 2006;Martínez-Checa et al, 2002), denitrifying (Peyton et al, 2001;Yoshie et al, 2006) or degrading aromatic compounds (García et al, 2004). …”

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confidence: 99%

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

González-Domenech

Martínez‐Checa

Quesada

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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We have made a polyphasic taxonomic study of strain 5CR T , isolated from Fuente de Piedra, Má laga, southern Spain. The strain is a moderately halophilic, Gram-negative rod, oxidase-positive and motile by a single polar flagellum. It does not produce acids from sugars and shows respiratory metabolism, using oxygen, nitrate and nitrite as terminal electron acceptors. It requires NaCl and grows best with 5-7.5 % w/v at temperatures of between 32 and 45 6C within a pH range of 6-8. Its 16S rRNA gene sequence indicates that strain 5CR T belongs to the genus Halomonas in the class Gammaproteobacteria. Its closest relatives are Halomonas alimentaria, H. nitroreducens, H. shengliensis and H. ventosae, with the type strains of which our strain showed 16S rRNA gene sequence similarity values of 96.7-97.8 %. DNA-DNA hybridization studies between strain 5CR T and H. ventosae CECT 5797 T , the phylogenetically nearest type strain, showed 40 % relatedness. Its G+C content is 65.7 mol%. Its major fatty acids are C 18 : 1 v7c (31.36 %), C 16 : 0 (25.55 %), C 16 : 1 v7c/iso-C 15 : 0 2-OH (23.23 %), C 19 : 0 cyclo v8c (8.14 %), C 12 : 0 3-OH (5.76 %) and C 10 : 0 (2.22 %) and the predominant respiratory lipoquinone is ubiquinone with nine isoprene units (Q-9). The proposed name for the novel species is Halomonas fontilapidosi sp. nov., strain 5CR T (5CECT 7341 T 5LMG 24455 T ) being the type strain.The family Halomonadaceae of the class Gammaproteobacteria comprises seven genera, Carnimonas, Chromohalobacter, Cobetia, Halomonas, Halotalea, Modicisalibacter and Zymobacter (Euzéby, 2008). Chromohalobacter, Cobetia, Halomonas and Modicisalibacter are composed of halophilic bacteria whereas Carnimonas, Halotalea and Zymobacter contain non-halophilic bacteria (Ben Ali Gam et al., 2007;Garrity et al., 2005;Ntougias et al., 2007). The genus Halomonas currently contains more than 50 species (Euzéby, 2008). Its members are Gram-negative, rodshaped, non-sporulated, aerobic chemo-organotrophs, with predominantly respiratory metabolism, using oxygen, nitrate or nitrite as electron acceptors. Few Halomonas species produce acids from sugars (see Mata et al., 2002;Dobson & Franzmann, 1996;Franzmann et al., 1988;Vreeland, 2005;Arahal et al., 2007). They are widely distributed throughout hypersaline environments. Some of them are recognized for their potential use in biotechnology (Margesin & Schinner, 2001;Ventosa & Nieto, 1995), producing exopolysaccharides (Arias et al., 2003;Mata et al., 2006;Martínez-Checa et al., 2002), denitrifying (Peyton et al., 2001; Yoshie et al., 2006) or degrading aromatic compounds (García et al., 2004).We have already described some novel halophilic taxa isolated from Fuente de Piedra (Málaga, Spain): Idiomarina fontislapidosi (Martínez-Cánovas et al., 2004c), Halomonas anticariensis (Martínez-Cánovas et al., 2004b) and strain 15CR of Halomonas cerina (González-Domenech et al., 2008a). Furthering our research into bacteria in this area, we subjected strain 5CR T to a polyphasic taxonomic investigation and as ...

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confidence: 99%

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

González-Domenech

Martínez‐Checa

Quesada

et al. 2009

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLO

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“…Mutagenesis in any one or more than one gene sequences might lead to constitutive expression of genes required for EPS production and thereby enhance the synthesis and accumulation of the EPS (Tang et al, 1991). Chemical mutagenesis in H. xianhensis remarkably increased the amount of EPS from 7.05 to 12.98 g L −1 , an amount much higher (4.9 g L −1 ) than that of the reported species of the genus Halomonas (Quesada et al, 1993;Arias et al, 2003;Mata et al, 2006). As against this, the EPS production of natural isolates of H. eurihalina ranged from 1.6-2.8 g L −1 (Bejar et al, 1998;Quesada et al, 1993).…”

Section: Discussionmentioning

confidence: 95%

Chemical Mutagenesis for Improvement of Production of a Biologically Active Extracellular Polymeric Substance by <i>Halomonas xianhensis</i> SUR308

Biswas¹,

Paul²

2016

American Journal of Microbiology

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A moderately halophilic bacterium, Halomonas xianhensis SUR308 (Genbank Accession No. KJ933394) isolated from solar saltern of Surala, Odisha, India was found to produce significant amount of a biologically active extracellular polymeric substances (EPS). Under optimized cultural conditions, the strain was able to produce 7.05 g L −1 of EPS after 8 days of incubation under shake culture. To enhance the EPS production efficiency, the isolate SUR308 was subjected to chemical mutagenesis following the use of hydroxylamine (HA), acridine orange (AO) and ethyl methanesulfonate (EMS). Evaluation of the toxicity of these mutagens indicated that EMS was comparatively toxic but effective for mutagenesis. Mutagenic treatments have resulted in the isolation of 58 amoxycillin resistant morphologically distinct phenotypes. Three of these mutant phenotypes (EM3, EM13 and HM6) were capable of producing 12.98, 11.97 and 11.21 g L −1 of EPS respectively in malt extract-yeast extract medium supplemented with casein hydrolysate with significant reduction of incubation period. Compared to wild type strain, mutant strains were found to produce 1.6-1.8 fold more EPS. However, the physico-chemical nature of the EPS derived from the mutants remained unaltered when compared with that of the wild type strain. These findings, therefore, have more impact on production economy of EPS for biotechnological applications.

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“…This can be addressed by chemical modification [30]. EPS from Halomonas maura and H. eurihalina has immunomodulating activity [31][32][33][34]. H. stenophila produced EPS having antitumor activity [35].…”

Section: Introductionmentioning

confidence: 99%

“…Halomonas is the potential genus producing EPS having efficient emulsifying activity among halophilic bacteria. Halomonas alkaliantartica, H. ventose, H. anticariencis and H. maura were found to produce EPS that are proposed to have a role as an emulsifying agent in oil recovery [36,28,23,32].…”

Section: Introductionmentioning

confidence: 99%

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

Deshmukh

Kanekar²,

Bhadekar

2017

Int J Pharm Pharm Sci

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Objective: To study 1) Optimization of nutritional and environmental parameters to enhance the yield of EPS by Halomonas smyrnensis SVD III isolated from seawater, West Coast of Maharashtra, India and 2) Purification and characterization of the EPS produced. Methods:The isolate was grown in Sehgal and Gibbons (SG) medium broth supplemented with 3% glucose, at 37 °C, 120 rpm for 7 d. Optimization of different parameters was carried out with one factor at a time approach. EPS was isolated from cell-free supernatant of the culture broth by centrifugation and precipitation using chilled ethanol, after removal of proteins by trichloroacetic acid (TCA) treatment. Characterization of the purified EPS was carried out with respect to fourier-transform infrared (FTIR) spectrum, 1 H nuclear magnetic resonance (NMR) spectrum and mass spectrometry (MS) analysis.Results: Two-fold increase in the yield of EPS (23 g/l) by the selected isolate was obtained by using culture conditions as 10% inoculum size having cell density of 10 7 cells/ml, pH 6, incubation temperature 45 °C, 3% carbohydrate, 0.5% yeast extract as nitrogen source, 20% salt concentration and 7 d of incubation period. Characterization of the purified EPS suggested the presence of dominated glycosidic linkages and heptasaccharide nature of the molecule. As the present strain is halophilic, 20% NaCl was found to be optimum. Conclusion:Optimization studies resulted in two-fold increase in the yield of EPS which is of heptasaccharide nature.

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Mauran, an exopolysaccharide produced by the halophilic bacterium Halomonas maura, with a novel composition and interesting properties for biotechnology

Cited by 178 publications

References 23 publications

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

Halomonas fontilapidosi sp. nov., a moderately halophilic, denitrifying bacterium

Chemical Mutagenesis for Improvement of Production of a Biologically Active Extracellular Polymeric Substance by <i>Halomonas xianhensis</i> SUR308

Production and Characterization of Exopolysaccharide From Marine Moderately Halophilic Bacterium Halomonas Smyrnensis SVD Iii

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