Maturation of the head of bacteriophage T4

Laemmli, Ulrich Karl; Quittner, S.F.

doi:10.1016/0042-6822(74)90409-7

Cited by 461 publications

(462 citation statements)

References 28 publications

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“…SDS-PAGE was carried out in 7-15% gradient minigels, using a discontinuous system (Laemmli and Favre, 1973). Western blotting to nitrocellulose membranes (Hybond-C extra from Amersham, Buckinghamshire, UK) was performed according to Towbin et al (1979).…”

Section: Gel Electrophoresis and Immunoblottingmentioning

confidence: 99%

Immunohistochemical localization of myosin va in the adult rat brain

et al. 2003

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Abstract-Brain myosin Va (MVa) is a molecular motor associated with plastic changes during development. MVa has previously been detected in the cell body and in dendrites of neuronal cells in culture, in cells of the guinea-pig cochlea, as well as in cerebellar cells. Adult Wistar rats (n‫,)41؍‬ 250 -300 g, were perfused with standard methods for immunohistochemistry, using a polyclonal, affinity-purified rabbit antibody against MVa tail domain. Anti-MVa antibody specifically stained neuronal nuclei from forebrain to cerebellar regions, and more intensely sensory nuclei. Differences in MVa immunoreactivity were detected between brain nuclei, ranging from very intense to weak staining. The analysis of MVa and glial fibrillary acidic protein staining in adjacent brain sections demonstrated a clear-cut neuronal labeling rather than an astroglial staining. The studies presented here represent a comprehensive map of MVa regional distribution in the CNS of the adult rat and may contribute to the basic understanding of its role in brain function and plasticity, particularly in relationship to phenomena that involve molecular motors, such as neurite outgrowth, organelle transport and neurotransmitter-vesicle cycling. It is important to highlight that this is a pioneer immunohistochemical study on the distribution of MVa on the whole brain of adult rats, a first step toward the understanding of its function in the CNS.

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Section: Gel Electrophoresis and Immunoblottingmentioning

confidence: 99%

Immunohistochemical localization of myosin va in the adult rat brain

et al. 2003

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“…Samples with 20-30 μg of protein per well, were separated on a 12% SDS gel [13]. After Western blotting [14], blots with primary antibodies (OLFM3 (Santa Cruz, Germany), Caspase-3 and FAK (BD, Europe, cat 610322 and 610087), phospho-FAK and phospho-PAX (Cell Signalling Cat 8556, 2541) or alpha-tubulin, LC3 (Sigma, cat T6199) were incubated overnight at 4°C.…”

Section: Western Blottingmentioning

confidence: 99%

Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line

Keenan¹,

Joyce²,

Aherne³

et al. 2012

Experimental Cell Research

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“…Lysis was achieved by freezing for 15 min at − 70°C and thawing [14]. One volume of double strength loading buffer [15] was added, the lysate vortexed 5 min, then heated for 3 min at 100°C and centrifuged for 2 min at 16 000 × g before separation by SDS-PAGE. The protein bands were transferred to nitrocellulose and tested with antibodies raised against the holoenzyme and against each subunit.…”

Section: Analysis Of Ftr Expressionmentioning

confidence: 99%

A dicistronic construct for the expression of functional spinach chloroplast ferredoxin:thioredoxin reductase in Escherichia coli

et al. 2000

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Ferredoxin:thioredoxin reductase (FTR) is a heterodimeric Fe S containing disulfide reductase involved in the light-dependent activation of photosynthetic enzymes. We have designed a dicistronic construct for the heterologous expression of this nucleus encoded chloroplast protein in Escherichia coli. The coding sequences for the two mature subunits have been inserted in tandem into the expression vector pET-3d. This dicistronic construct is correctly translated yielding soluble, perfectly functional FTR. The recombinant enzyme is composed of both subunits, contains the correctly inserted Fe S cluster as evidenced by its spectral properties and is indistinguishable from the enzyme isolated from leaves in its capacity to activate chloroplast fructose-1,6-bisphosphatase, one of the well known light activated enzymes of the Calvin cycle.

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Maturation of the head of bacteriophage T4

Cited by 461 publications

References 28 publications

Immunohistochemical localization of myosin va in the adult rat brain

Immunohistochemical localization of myosin va in the adult rat brain

Olfactomedin III expression contributes to anoikis-resistance in clonal variants of a human lung squamous carcinoma cell line

A dicistronic construct for the expression of functional spinach chloroplast ferredoxin:thioredoxin reductase in Escherichia coli

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