Maturation of Lobster Stomatogastric Ganglion Rhythmic Activity

Richards, Kathryn S.; Miller, William L.; Marder, Eve

doi:10.1152/jn.1999.82.4.2006

Cited by 30 publications

(20 citation statements)

References 22 publications

(22 reference statements)

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“…Receptor and channel density regulation must be appropriately matched to the increase in membrane surface area, and synaptic function must be altered in accordance with changes in input resistance in the postsynaptic cells (Edwards et al, 1994b). The STG has the same number of neurons in embryos and adults (Casasnovas and Meyrand, 1995), and the characteristic triphasic pattern and the regularity of the pyloric rhythm emerge over the course of larval development (Richards et al, 1999). We show here that the STG grows substantially from juvenile to adult animal.…”

Section: Stability Of Network Output Across Animals and During Growthmentioning

confidence: 56%

Animal-to-Animal Variability in Motor Pattern Production in Adults and during Growth

Bucher¹,

Prinz²,

Marder³

2005

J. Neurosci.

175

196

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Which features of network output are well preserved during growth of the nervous system and across different preparations of the same size? To address this issue, we characterized the pyloric rhythms generated by the stomatogastric nervous systems of 99 adult and 12 juvenile lobsters (Homarus americanus). Anatomical studies of single pyloric network neurons and of the whole stomatogastric ganglion (STG) showed that the STG and its neurons grow considerably from juvenile to adult. Despite these changes in size, intracellularly recorded membrane potential waveforms of pyloric network neurons and the phase relationships in the pyloric rhythm were very similar between juvenile and adult preparations. Across adult preparations, the cycle period and number of spikes per burst were not tightly maintained, but the mean phase relationships were independent of the period of the rhythm and relatively tightly maintained across preparations. We interpret this as evidence for homeostatic regulation of network activity.

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Section: Stability Of Network Output Across Animals and During Growthmentioning

confidence: 56%

Animal-to-Animal Variability in Motor Pattern Production in Adults and during Growth

Bucher¹,

Prinz²,

Marder³

2005

J. Neurosci.

175

196

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“…However, recent work has emphasized that virtually undistinguishable motor patterns can nevertheless be produced by different ion channels and synaptic combinations within a given network (Prinz et al, 2004). Therefore, we cannot exclude that the combination of expressed channels is different in embryonic and adult STG neurons as suggested in Homarus americanus (Richards et al, 1999;Richards and Marder, 2000), although both can express either embryonic-or adult-like motor patterns. Finally, the presence of gap junctions could allow the sharing of conductances across several cells, one cell being strongly affected by the currents expressed by electrically coupled cells.…”

Section: Network Reconfiguration As a Developmental Mechanismmentioning

confidence: 84%

Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network

Ducret¹,

Feuvre²,

Meyrand³

et al. 2007

J. Neurosci.

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The maturation and operation of neural networks are known to depend on modulatory neurons. However, whether similar mechanisms may control both adult and developmental plasticity remains poorly investigated. To examine this issue, we have used the lobster stomatogastric nervous system (STNS) to investigate the ontogeny and role of GABAergic modulatory neurons projecting to small pattern generating networks. Using immunocytochemistry, we found that modulatory input neurons to the stomatogastric ganglion (STG) express GABA only after metamorphosis, a time that coincides with the developmental switch from a single to multiple pattern generating networks within the STNS. We demonstrate that blocking GABA synthesis with 3-mercapto-propionic acid within the adult modulatory neurons results in the reconfiguration of the distinct STG networks into a single network that generates a unified embryoniclike motor pattern. Using dye-coupling experiments, we also found that gap-junctional coupling is greater in embryos and GABAdeprived adults exhibiting the unified motor pattern compared with control adults. Furthermore, GABA was found to diminish directly the extent and strength of electrical coupling within adult STG networks. Together, these observations suggest the acquisition of a GABAergic phenotype by modulatory neurons after metamorphosis may induce the reconfiguration of the single embryonic network into multiple adult networks by directly decreasing electrical coupling. The findings also suggest that adult neural networks retain the ability to express typical embryonic characteristics, indicating that network ontogeny can be reversed and that changes in electrical coupling during development may allow the segregation of multiple distinct functional networks from a single large embryonic network.

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“…8,9 The progressive increases in the frequency and regularity of the rhythms coincide well with the gradually increased amount of tachykinin neuropeptides detected by MS. It is possible that besides descending neuromodulatory input, many other factors like synaptic connections and/or cellular properties of the network itself could also influence output maturation.…”

Section: ■ Results and Discussionmentioning

confidence: 57%

Mass Spectral Charting of Neuropeptidomic Expression in the Stomatogastric Ganglion at Multiple Developmental Stages of the Lobster Homarus americanus

Jiang

Chen

Wang

et al. 2012

ACS Chem. Neurosci.

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ABSTRACT:The stomatogastric nervous system (STNS) of the American lobster Homarus americanus serves as a useful model for studies of neuromodulatory substances such as peptides and their roles in the generation of rhythmic behaviors. As a central component of the STNS, the stomatogastric ganglion (STG) is rich in neuropeptides and contains welldefined networks of neurons, serving as an excellent model system to study the effect of neuropeptides on the maturation of neural circuits. Here, we utilize multiple mass spectrometry (MS)-based techniques to study the neuropeptide content and abundance in the STG tissue as related to the developmental stage of the animal. Capillary electrophoresis (CE)-MS was employed to unambiguously identify low abundance neuropeptide complements, which were not fully addressed using previous methods. In total, 35 neuropeptides from 7 different families were detected in the tissue samples. Notably, 10 neuropeptides have been reported for the first time in this study. In addition, we utilized a relative quantitation method to compare neuropeptidomic expression at different developmental stages and observed sequential appearance of several neuropeptides. Multiple isoforms within the same peptide family tend to show similar trends of changes in relative abundance during development. We also determined that the relative abundances of tachykinin peptides increase as the lobster grows, suggesting that the maturation of circuit output may be influenced by the change of neuromodulatory input into the STG. Collectively, this study expands our knowledge about neuropeptides in the crustacean STNS and provides useful information about neuropeptide expression in the maturation process.

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Maturation of Lobster Stomatogastric Ganglion Rhythmic Activity

Cited by 30 publications

References 22 publications

Animal-to-Animal Variability in Motor Pattern Production in Adults and during Growth

Animal-to-Animal Variability in Motor Pattern Production in Adults and during Growth

Removal of GABA within Adult Modulatory Systems Alters Electrical Coupling and Allows Expression of an Embryonic-Like Network

Mass Spectral Charting of Neuropeptidomic Expression in the Stomatogastric Ganglion at Multiple Developmental Stages of the Lobster Homarus americanus

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