Maturation of Layer V Pyramidal Neurons in the Rat Prefrontal Cortex: Intrinsic Properties and Synaptic Function

Zhang, Zhong-wei

doi:10.1152/jn.00855.2003

Cited by 185 publications

(211 citation statements)

References 58 publications

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“…Consistent with our findings for Kv-mediated currents, the most dramatic changes in AP parameters occur between weeks 1 and 3, approaching adult values by weeks 3-5 (Lorenzon and Foehring 1993;McCormick and Prince 1987;Zhang 2004). Because of its relatively hyperpolarized activation range and fast kinetics (Foehring and Surmeier 1993;Yuan et al 2005), the maturation of A-type current would be expected to lead to more rapid spike repolarization and narrower spikes.…”

Section: Development Of Excitability and Firing Patternssupporting

confidence: 85%

“…Pyramidal cell resting membrane potential (RMP) becomes more hyperpolarized with postnatal development and input resistance and membrane time constant decrease Beique et al 2004;Christophe et al 2005;Kasper et al 1994aKasper et al , 1994bLorenzon and Foehring 1993;Maravall et al 2004a;McCormick and Prince 1987;Metherate and Aramkis 1999;Oswald and Reyes 2008;Tyzio et al 2003). Repetitive firing behavior also changes with age Christophe et al 2005;Kasper et al 1994aKasper et al , 1994bLorenzon and Foehring 1993;Maravall et al 2004aMaravall et al , 2004bMcCormick and Prince 1987;Metherate and Aramkis 1999;Oswald and Reyes 2008;van Brederode et al 2000;Zhang 2004). In mature pyramidal neurons, voltage-gated K ϩ channels contribute to the filtering and integration of synaptic inputs, as well as shaping firing rates and pattern (Guan et al 2007a;Higgs and Spain 2009;Schwindt et al 1988).…”

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confidence: 99%

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Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Guan

Horton

Armstrong

et al. 2011

Journal of Neurophysiology

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Guan D, Horton LR, Armstrong WE, Foehring RC. Postnatal development of A-type and Kv1-and Kv2-mediated potassium channel currents in neocortical pyramidal neurons. J Neurophysiol 105: 2976-2988, 2011. First published March 30, 2011 doi:10.1152/jn.00758.2010.-Potassium channels regulate numerous aspects of neuronal excitability, and several voltage-gated K ϩ channel subunits have been identified in pyramidal neurons of rat neocortex. Previous studies have either considered the development of outward current as a whole or divided currents into transient, A-type and persistent, delayed rectifier components but did not differentiate between current components defined by ␣-subunit type. To facilitate comparisons of studies reporting K ϩ currents from animals of different ages and to understand the functional roles of specific current components, we characterized the postnatal development of identified Kv channel-mediated currents in pyramidal neurons from layers II/III from rat somatosensory cortex. Both the persistent/slowly inactivating and transient components of the total K ϩ current increased in density with postnatal age. We used specific pharmacological agents to test the relative contributions of putative Kv1-and Kv2-mediated currents (100 nM ␣-dendrotoxin and 600 nM stromatoxin, respectively). A combination of voltage protocol, pharmacology, and curve fitting was used to isolate the rapidly inactivating A-type current. We found that the density of all identified current components increased with postnatal age, approaching a plateau at 3-5 wk. We found no significant changes in the relative proportions or kinetics of any component between postnatal weeks 1 and 5, except that the activation time constant for A-type current was longer at 1 wk. The putative Kv2-mediated component was the largest at all ages. Immunocytochemistry indicated that protein expression for Kv4.2, Kv4.3, Kv1.4, and Kv2.1 increased between 1 wk and 4 -5 wk of age. Kv channel; somatosensory cortex; voltage clamp; A current; delayed rectifier AT BIRTH, the rodent somatosensory system is immature, and somatosensory cortex undergoes dramatic changes over the first postnatal month. Neocortical pyramidal cells are generated prenatally in an "inside-out" laminar pattern (Caviness 1982; Caviness et al.

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Section: Development Of Excitability and Firing Patternssupporting

confidence: 85%

mentioning

confidence: 99%

Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Guan

Horton

Armstrong

et al. 2011

Journal of Neurophysiology

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“…In human studies, McKinstry et al (2002) reported that the time course of anisotropy diminishment coincides with the period of "cortical maturation" (Sidman and Rakic, 1982) and attributed the decrease to the growth of dendrites, as well as formation of cell processes and their connections, to the arrival of local circuit neurons and their differentiation, and to the disappearance of the radial glia (Sidman and Rakic, 1973;Kostovic and Rakic, 1990). In rat brains, axonal and dendritic branching in the cortex begins at approximately P1-P7, and the peak of synapse formation occurs at approximately P7-P14, whereas dendrite growth extends to the third week (Miller, 1981;Miller and Peters, 1981;Harris et al, 1992;Wong and Ghosh, 2002;Zhang, 2004). …”

Section: Discussionmentioning

confidence: 99%

Quantitative Cortical Mapping of Fractional Anisotropy in Developing Rat Brains

Huang¹,

Yamamoto²,

Hossain³

et al. 2008

J. Neurosci.

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Cortical development is associated with a series of events that involve axon and dendrite growth and synaptic formation. Although these developmental processes have been investigated in detail with histology, three-dimensional and quantitative imaging methods for rodent brains may be useful for genetic and pharmacological studies in which cortical developmental abnormalities are suspected. It has been shown that diffusion tensor imaging (DTI) can delineate the columnar organization of the fetal and early neonatal cortex based on a high degree of diffusion anisotropy along the columnar structures. This anisotropy is known to decrease during brain development. In this study, we applied DTI to developing rat brains at five developmental stages, postnatal days 0, 3, 7, 11 and 19, and used diffusion anisotropy as an index to characterize the structural change. Statistical analysis reveals four distinctive cortical areas that demonstrate a characteristic time course of anisotropy loss. This method may provide a means to delineate specific cortical areas and a quantitative method to detect abnormalities in cortical development in rodent pathological models.

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“…As described previously by Zhang (2004), slices possessing biocytin-filled cells were carefully removed from the recording chamber and fixed by immersion in 4% paraformaldehyde in 0.1 M PBS overnight at 4°C, followed by two rinses in PBS (0.1 M). The slices were then incubated in 3% H 2 O 2 and 10% methanol in PBS (0.1 M) for 1 h at room temperature to quench endogenous peroxidase activity, which was followed by five 5 min washes in PBS plus 0.1% Triton.…”

Section: Methodsmentioning

confidence: 99%

GABAergic Synaptic Inhibition Is Reduced before Seizure Onset in a Genetic Model of Cortical Malformation

Trotter¹,

Kapur²,

Anzivino³

et al. 2006

J. Neurosci.

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Malformations of the neocortex are a common cause of human epilepsy; however, the critical issue of how disturbances in cortical organization render neurons epileptogenic remains controversial. The present study addressed this issue by studying inhibitory structure and function before seizure onset in the telencephalic internal structural heterotopia (tish) rat, which is a genetic model of heightened seizure susceptibility associated with a prominent neocortical malformation. Both normally positioned (normotopic) and misplaced (heterotopic) pyramidal neurons in the tish neocortex exhibited lower resting membrane potentials and a tendency toward higher input resistance compared with pyramidal neurons from control brains. GABAergic synaptic transmission was attenuated in the tish cortex, characterized by significant reductions in the frequency of spontaneous IPSCs (sIPSCs) and miniature IPSCs recorded from pyramidal neurons. In addition, the amplitudes of sIPSCs were reduced in the tish neocortex, an effect that was more profound in the normotopic cells. Immunohistochemical assessment of presynaptic GABAergic terminals showed a reduction in terminals surrounding pyramidal cell somata in normotopic and heterotopic tish neocortex. The attenuation of inhibitory innervation was more prominent for normotopic neurons and was associated with a reduction in a subset of GABAergic interneurons expressing the calcium-binding protein parvalbumin. Together, these findings indicate that key facets of inhibitory GABAergic neurotransmission are disturbed before seizure onset in a brain predisposed to developing seizures. Such alterations represent a rational substrate for reduced seizure thresholds associated with certain cortical malformations.

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Maturation of Layer V Pyramidal Neurons in the Rat Prefrontal Cortex: Intrinsic Properties and Synaptic Function

Cited by 185 publications

References 58 publications

Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Postnatal development of A-type and Kv1- and Kv2-mediated potassium channel currents in neocortical pyramidal neurons

Quantitative Cortical Mapping of Fractional Anisotropy in Developing Rat Brains

GABAergic Synaptic Inhibition Is Reduced before Seizure Onset in a Genetic Model of Cortical Malformation

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