Maturation of alfalfa (Medicago sativa L.) somatic embryos by abscisic acid, sucrose and chilling stress

Lecouteux, Claude G.; Lai, Fang-Ming; McKersie, Bryan D.

doi:10.1016/0168-9452(93)90021-q

Cited by 54 publications

(22 citation statements)

References 21 publications

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“…ABA application (20 lM) resulted in its highly increased content in mature embryos. This fact prevented their precocious germination and allowed the induction of desiccation tolerance (Senaratna et al , 1990Lecouteux et al 1993;Ruduś et al 2006). Thus, the pattern of ABA accumulation during the formation and maturation of somatic embryos in this regeneration system resembles that observed in zygotic embryos (Finkelstein et al 2002).…”

Section: Discussionmentioning

confidence: 79%

Do stress-related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis?

2009

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This study examined the role of endogenous abscisic acid (ABA) and jasmonic acid (JA) in indirect somatic embryogenesis of Medicago sativa L. A multiplex GC-MS/MS technique allowed quantitative single-run analyses of ABA, JA, 12-oxophytodienoic acid (OPDA) and indole-3-acetic acid (IAA). The preparation of initial explants led to a strong accumulation of ABA, JA and OPDA but not of IAA. Substantially higher levels of ABA, JA and OPDA were detected in developing somatic embryos than in callus or embryogenic suspension. Fluridone (FLD) decreased ABA, JA and OPDA levels. Indoprofen (INP) appeared to be a specific inhibitor of octadecanoid biosynthesis. Somatic embryo production and development were negatively affected by FLD or INP. Only INP (0.5 lM) applied during proliferation phase increased the number of cotyledonary embryos. The results strongly indicate the involvement of ABA and JA in somatic embryogenesis of M. sativa. Surprisingly, low IAA contents in comparison to stress-related compounds (ABA, JA and OPDA) were detected in explants, embryogenic tissues and somatic embryos.

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Section: Discussionmentioning

confidence: 79%

Do stress-related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis?

2009

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“…These findings indicate sugar and ABA, both related to stress, might also be related to the induction of somatic embryos. Many data confirm the effect of applied ABA during the late embryogenesis -stages of embryo maturation and conversion (Lecouteux et al, 1993;Mhaske et al, 1998) but there is no data concerning the application of exogenous ABA during the induction stage.…”

Section: Introductionmentioning

confidence: 92%

Embryo induction and regeneration from root explants of Medicago truncatula after osmotic pre-treatment

Iantcheva

Slavov

Prinsen

et al. 2005

Plant Cell Tiss Organ Cult

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Embryo induction and regeneration from suspension culture of two Medicago truncatula cvs. (cv. R 108 1 and cv. Jemalong) have been studied. The influence of osmotic pre-treatment (1 M solution of sucrose for 48 h and 72 h) of roots as an initial explant, on embryogenic efficiency of the suspension culture was assessed. In comparison to the control, the level of abscisic acid (ABA) increased significantly after osmotic stress. The increased ABA level did not correlate with the induction of embryogenesis neither with the improved embryogenic potential of cv. R 108 1. The shortest regeneration period and the highest percent of conversion to plants were found in cv. R 108 1 after 72-h pre-treatment of roots. The efficiency of somatic embryo conversion was less after 48-h pre-treatment and much less for the untreated control. Osmotic stress did not positively affect the process of embryogenesis from root explants of cv. Jemalong, confirming its cultivar dependence. A single cell suspension fraction was produced in both Medicago trunacatula cvs. during the somatic embryo maturation stage. A higher embryogenic potential than the initial suspension culture was established only for the cell suspension originating from 72-h pre-treated roots of cv. R 108 1. The data confirms that the process of somatic embryo induction and embryo conversion from root explants of cv. R 108 1 could be promoted by osmotic stress pre-treatment.

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“…Jacobsen (1987, 1990) searched for protein markers associated with embryogenic differentiation in pea callus cultures studying also the potential role of soluble auxin receptors (proteins binding specifically active auxins) in auxininduced pea somatic embryogenesis (Jacobsen 1991). Only a conference abstract is available on pea somatic embryos storage proteins (Lehminger-Mertens et al 1990). The authors found roughly the same protein profiles for both somatic and zygotic embryos deducing almost analogous development of both embryo types.…”

Section: Introductionmentioning

confidence: 99%

“…The main portion of knowledge accumulated so far has been obtained with alfalfa (Stuart et al 1988, Krochko et al 1992, 1994, Lai et al 1992, Lecouteux et al 1993, Lai and Mckersie 1994a and soybean (Christou and Yang 1989, Slawinska and Obendorf 1991, Komatsuda et al 1992, Dahmer et al 1992, Stejskal and Griga 1995, Chanprame and Widholm 1996, which represent traditional experimental models for somatic embryogenesis in forage and grain legumes.…”

Section: Introductionmentioning

confidence: 99%

Protein patterns associated with Pisum sativum somatic embryogenesis

Griga¹,

Horáček²,

Klenotičová³

2007

Biol. plant.

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Total protein patterns were studied in the course of development of pea somatic embryos using simple protocol of direct regeneration from shoot apical meristems on auxin supplemented medium. Protein content and total protein spectra (SDS-PAGE) of somatic embryos in particular developmental stages were analysed in Pisum sativum, P. arvense, P. elatius and P. jomardi. Expression of seed storage proteins in somatic embryos was compared with their accumulation in zygotic embryos of selected developmental stages. Pea vegetative tissues, namely leaf and root, were used as a negative control not expressing typical seed storage proteins. The biosynthesis and accumulation of seed storage proteins was observed during somatic embryo development (since globular stage), despite of the fact that no special maturation treatment was applied. Major storage proteins typical for pea seed (globulins legumin, vicilin, convicilin and their subunits) were detected in somatic embryos. In general, the biosynthesis of storage proteins in somatic embryos was lower as compared to mature dry seed. However, in some cases the cotyledonary somatic embryos exhibited comparatively high expression of vicilin, convicilin and pea seed lectin, which was even higher than those in immature but morphologically fully developed zygotic embryos. Desiccation treatments did not affect the protein content of somatic embryos. The transfer of desiccated somatic embryos on hormone-free germination medium led to progressive storage protein degradation. The expression of true seed storage proteins may serve as an explicit marker of somatic embryogenesis pathway of regeneration as well as a measure of maturation degree of somatic embryos in pea.

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Maturation of alfalfa (Medicago sativa L.) somatic embryos by abscisic acid, sucrose and chilling stress

Cited by 54 publications

References 21 publications

Do stress-related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis?

Do stress-related phytohormones, abscisic acid and jasmonic acid play a role in the regulation of Medicago sativa L. somatic embryogenesis?

Embryo induction and regeneration from root explants of Medicago truncatula after osmotic pre-treatment

Protein patterns associated with Pisum sativum somatic embryogenesis

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