Maturation in Vitro of Human Ovarian Oocytes

Edwards, Robert G.

doi:10.1016/s0140-6736(65)92903-x

Cited by 367 publications

(131 citation statements)

References 7 publications

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“…Early in 1965, Robert G. Edwards discovered that morphologically immature human oocytes could spontaneously mature in vitro and be subsequently fertilized [5]. Eighteen years later, the first in vitro maturation (IVM) baby came into this world [30].…”

Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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Purpose Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM). Methods Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10 −2 , 1, 10 2 , 10 4 , 10 6 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture. Results The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P<0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P<0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (10 6 nM). Conclusions The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.

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Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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“…The introduction of in vitro maturation in human reproduction represented a valuable choice of protocol, when dealing with patients at risk of ovarian hyperstimulation using exogenous gonadotrophins for classical AR technologies. Since the first report of human oocyte IVM [6], the methodology has been refined and considerable progress has been achieved in terms of oocyte maturation and pregnancy rates.…”

Section: Introductionmentioning

confidence: 99%

Fertilization outcome, embryo development and birth after unstimulated IVM

Bös-Mikich

Ferreira²,

Höher³

et al. 2010

J Assist Reprod Genet

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Background/Purposes A common observation in oocyte in vitro maturation (IVM) cycles is poor embryo quality. However, no study was dedicated to assess zygote and early cleavage embryo quality in IVM cycles. The objective of this study is to analyze fertilization outcome, embryo development and the resulting pregnancy and births in unstimulated IVM cycles. Methods IVM oocytes were collected 36 h post hCG and matured in vitro for 28-30 h. All oocytes were inseminated by ICSI. Resulting zygotes and embryos were assessed on day-1, 2 and 3, when transfers were made. Results The overall oocyte maturation and fertilization rates were 63% and 62%, respectively. Abnormal fertilization rate was 1.7%. Ninety five and 14.6% of the 2Pn zygotes reached the 2-cell and 8 -cell stage at day-2 and day-3, respectively. Embryo quality assessment on day-3 at transfer revealed that only 9% of the embryos were of very good quality. Most embryos showed developmental delay. An average of 3.29 embryos were transferred per patient resulting in implantation and clinical gestation rates of 16% and 32%, respectively. Overall 14 healthy babies were born and there is one ongoing pregnancy. ConclusionResults show a significant rate of abnormal fertilization and poor embryo quality after IVM, which is reflected in a higher than average number of embryos being transferred. However, pregnancy, implantation and birth rates are reasonably high and allow us to consider IVM a valuable approach for the treatment of infertility in PCO or PCOS patients.

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“…Edwards (Edwards, 1965;Steptoe and Edwards, 1978). Indeed, Edwards is a giant of human ART and science in general.…”

Section: Clinical Regimens In Human In Vitro Maturationmentioning

confidence: 99%

Human oocyte maturation in vitro

Coticchio¹,

Canto²,

Guglielmo³

et al. 2012

Int. J. Dev. Biol.

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Oocytes from medium-sized antral follicles have already completed their growth phase and, if released from the follicular environment and cultured in vitro, are able to resume the meiotic process and mature. However, in vitro maturation (IVM) does not entirely support all the nuclear and cytoplasmic changes that occur physiologically as an effect of the ovulatory stimulus. Regardless, oocyte IVM is widely applied for the breeding of agriculturally important species. In assisted reproduction technology, IVM has been proposed as an alternative treatment to circumvent the drawbacks of standard ovarian stimulation regimens. Initially introduced to eliminate the risks of ovarian hyperstimulation syndrome afflicting women presenting with polycystic ovaries, subsequently IVM has been suggested to represent an additional approach suitable also for normovulatory patients. So far, in children born from IVM cycles, no doubts of an increased incidence of congenital abnormalities have been raised. Many more births would be achieved if novel IVM systems, currently dominated by empiricism, could be conceived according to more physiological criteria. Recent findings shedding new light on the control of meiotic progression, the support of cumulus cells to the oocyte cellular reorganization occurring during maturation, and the modulation of the stimulus that promotes oocyte maturation downstream the mid-cycle gonadotropin signal are likely to provide crucial hints for the development of more efficient IVM systems.

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Maturation in Vitro of Human Ovarian Oocytes

Cited by 367 publications

References 7 publications

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Fertilization outcome, embryo development and birth after unstimulated IVM

Human oocyte maturation in vitro

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