Maturation-dependent change and regional variations in acoustic stiffness of rabbit articular cartilage: an examination of the superficial collagen-rich zone of cartilage

Kuroki, Hiroshi; Nakagawa, Yasuaki; Mori, Koji; Kobayashi, Masanori; Yasura, Ko; Okamoto, Yukihiro; Mizuno, Yukio; Ando, Keiji; Ikeuchi, Ken; Nakamura, Takashi

doi:10.1016/j.joca.2006.02.001

Cited by 19 publications

(20 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…They found no correlation with age and no peak at skeletal maturity, but they also did not have as many time points as our study. We measured a peak in shear modulus at skeletal maturity (9 weeks) similar to the peak of instantaneous modulus of elasticity found by Julkunen et al (2009) and Kuroki et al (2006) in rabbits. Also similar to our results, it has been previously shown that the medial femoral condyle and tibial plateau are stiffer than lateral in the rabbit cartilage (Räsänen and Messner 1996).…”

Section: Discussionsupporting

confidence: 70%

Permeability and shear modulus of articular cartilage in growing mice

Oyen

2015

Biomech Model Mechanobiol

View full text Add to dashboard Cite

Articular cartilage maturation is the postnatal development process that adapts joint surfaces to their site-specific biomechanical demands. Understanding the changes in mechanical tissues properties during growth is a critical step in advancing strategies for orthopedics and for cell- and biomaterial- based therapies dedicated to cartilage repair. We hypothesize that at the microscale, the articular cartilage tissue properties of the mouse (i.e., shear modulus and permeability) change with the growth and are dependent on location within the joint. We tested cartilage on the medial femoral condyle and lateral femoral condyle of seven C57Bl6 mice at different ages (2, 3, 5, 7, 9, 12, and 17 weeks old) using a micro-indentation test. Results indicated that permeability decreased with age from 2 to 17 weeks. Shear modulus reached a peak at the end of the growth (9 weeks). Within an age group, shear modulus was higher in the MFC than in the LFC, but permeability did not change. We have developed a method that can measure natural alterations in cartilage material properties in a murine joint, which will be useful in identifying changes in cartilage mechanics with degeneration, pathology, or treatment.

show abstract

Section: Discussionsupporting

confidence: 70%

Permeability and shear modulus of articular cartilage in growing mice

Oyen

2015

Biomech Model Mechanobiol

View full text Add to dashboard Cite

show abstract

“…Although differentiating between healthy cartilage and ICRS grade 1 cartilage may be difficult using mechanical testing alone [ 17 ], a differentiation could be detected using ultrasound. The ultrasound evaluation is performed within a very short time (<0.5 s) [ 20 ].…”

Section: Discussionmentioning

confidence: 99%

“…The echo duration of Group N is a parameter related to the surface irregularity of cartilage. See [ 20 , 30 , 31 ].…”

Section: Methodsmentioning

confidence: 99%

Ultrasound properties of articular cartilage in the tibio-femoral joint in knee osteoarthritis: relation to clinical assessment (International Cartilage Repair Society grade)

et al. 2008

View full text Add to dashboard Cite

show abstract

“…The correlation of ultrasound reflection to collagen or proteoglycan content is previously reported [19], [20], [21]. Moreover, we and other groups reported that the reflected ultrasound waves provide superficial information of the cartilage [19], [22], [23]. The numerical analysis of ultrasonic propagation in articular cartilage from another study of our group suggests that the collagen content from the surface to one wave length (1600[m/s]/10 MHz = 0.16 mm) is correlated with US signal intensity [24].…”

Section: Discussionmentioning

confidence: 84%

Ultrasound Can Detect Macroscopically Undetectable Changes in Osteoarthritis Reflecting the Superficial Histological and Biochemical Degeneration: Ex Vivo Study of Rabbit and Human Cartilage

et al. 2014

View full text Add to dashboard Cite

Recognizing subtle cartilage changes in the preclinical stage of osteoarthritis (OA) is essential for early diagnosis. To this end, the ability of the ultrasound signal intensity to detect macroscopically undetectable cartilage change was investigated. In this study, cartilage of rabbit OA model and human OA samples was examined by macroscopic evaluation, ultrasound signal intensity, histology with Mankin scores, and Fourier transform infrared imaging (FTIRI) analysis. Rabbit OA was induced by anterior cruciate ligament transection and evaluated at 1, 2, 4 and 12 weeks. Twenty human samples were harvested during total knee arthroplasty from OA patients who had macroscopically normal human cartilage (ICRS grade 0) on the lateral femoral condyle. In the animal study, there was no macroscopic OA change at 2 weeks, but histology detected degenerative changes at this time point. Ultrasound signal intensity also detected degeneration at 2 weeks. In human samples, all samples were obtained from macroscopically intact site, however nearly normal (0≤ Mankin score <2), early OA (2≤ Mankin score <6), and moderate OA (6≤ Mankin score <10) samples were actually intermixed. Ultrasound signal intensity was significantly different among these 3 stages and was well correlated with Mankin scores (R = −0.80) and FTIR parameters related to collagen and proteoglycan content in superficial zone. In conclusion, ultrasound can detect microscopic cartilage deterioration when such changes do not exist macroscopically, reflecting superficial histological and biochemical changes.

show abstract

Maturation-dependent change and regional variations in acoustic stiffness of rabbit articular cartilage: an examination of the superficial collagen-rich zone of cartilage

Abstract: The response of articular cartilage to ultrasound was maturation-dependent. Acoustic properties differed from mechanical stiffness properties, which were determined using indentation. Ultrasound may detect properties of the surface collagen of the articular cartilage.

Cited by 19 publications

References 42 publications

Permeability and shear modulus of articular cartilage in growing mice

Permeability and shear modulus of articular cartilage in growing mice

Ultrasound properties of articular cartilage in the tibio-femoral joint in knee osteoarthritis: relation to clinical assessment (International Cartilage Repair Society grade)

Ultrasound Can Detect Macroscopically Undetectable Changes in Osteoarthritis Reflecting the Superficial Histological and Biochemical Degeneration: Ex Vivo Study of Rabbit and Human Cartilage

Contact Info

Product

Resources

About