Maturation conditions, post-ovulatory age, medium pH, and ER stress affect [Ca2+]i oscillation patterns in mouse oocytes

Yuan, Ruiying; Wang, Feng; Li, Sen; Ma, Jun‐Yu; Guo, Lei; Li, Xiaolong; Zhu, Haijing; Xie, Feng; Li, Qiannan; Zhou, Qian; Lin, Zi-Bin; Schatten, Heide; Ou, Xiang‐Hong

doi:10.1007/s10815-021-02100-9

Cited by 6 publications

(4 citation statements)

References 49 publications

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“…In rat, Premkumar and Chaube demonstrated that an insufficient increase in free intracellular Ca 2+ induces POAderived abortive spontaneous egg activation (SEA) in a timedependent fashion (~ 60% after 17-h post-hCG injection, and 100% after 19 h) [8]. The importance of intracellular Ca 2+ level is corroborated by recent studies that found abnormal Ca 2+ oscillations in mouse POA-oocytes compared to fresh ovulated oocytes [57,58], correlating it to higher fragmentation and altered mt membrane permeability which leads to the release of proapoptotic factors [57]. Specifically, the source of Ca 2+ -impaired homeostasis is linked to ER-specific ATPase activity suppression, depletion of Ca 2+ levels in ER [59], and inositol 1,4,5-trisphosphate (IP 3 )-dependent mechanisms misfunction [60].…”

Section: Intracellular Ca 2+ Homeostasismentioning

confidence: 86%

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Nisio

Antonouli

Damdimopoulou

et al. 2022

J Assist Reprod Genet

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In mammalian species an optimal fertilization window during which successful fertilization occurs. In the majority of mammals estrus marks ovulation time and coincident with mating, thereby allowing the synchronized meeting in the fallopian tubes, between freshly ejaculated sperm and freshly ovulated oocytes. Conversely, women do not show natural visual signs of ovulation such that fertilization can occur hours later involving an aged oocyte and freshly ejaculated spermatozoa. During this time, the oocyte undergoes a rapid degradation known as “postovulatory aging” (POA). POA may become particularly important in the human-assisted reproductive technologies, as the fertilization of retrieved mature oocytes can be delayed due to increased laboratory workload or because of unforeseeable circumstances, like the delayed availability of semen samples. This paper is an updated review of the consequences of POA, either in vivo or in vitro, on oocyte quality with particular attention to modifications caused by POA on oocyte nuclear, cytoplasmic, genomic, and epigenetic maturation, and embryo development.

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Section: Intracellular Ca 2+ Homeostasismentioning

confidence: 86%

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Nisio

Antonouli

Damdimopoulou

et al. 2022

J Assist Reprod Genet

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“…2,3 Oocytes remain in the body for up to several decades and are therefore easily damaged by the environment or external stimuli such as a pH change and reactive oxygen species (ROS) generation. [4][5][6] These factors reduce the quality and genetic stability of oocytes. 7,8 Exposure of cells to a variety of external and internal stimuli can induce severe DNA damage.…”

Section: Introductionmentioning

confidence: 99%

Melatonin loaded PLGA nanoparticles effectively ameliorate the in vitro maturation of deteriorated oocytes and the cryoprotective abilities during vitrification process

et al. 2023

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“…During fertilization, sperm entry induces Ca 2+ oscillations and activates oocytes ( 36 ). The significant elevation of Ca 2+ in the GV-VI group may interfere with this Ca 2+ oscillation, resulting in insufficient oocyte activation and reduced developmental competence ( 37 , 38 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence

et al. 2023

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IntroductionAcquisition of germinal vesicle (GV) stage oocytes for fertility preservation (FP) offers several benefits over in vivo matured oocyte cryopreservation following ovarian stimulation, particularly for cancer patients necessitating immediate treatment. Two FP approaches for GV oocytes are available: vitrification before in vitro maturation (IVM) at the GV stage (GV-VI) or post-IVM at the metaphase II (MII) stage (MII-VI). The optimal method remains to be determined.MethodsIn this study, mouse oocytes were collected without hormonal stimulation and vitrified either at the GV stage or the MII stage following IVM; non-vitrified in vitro matured MII oocytes served as the control (CON). The oocyte quality and developmental competence were assessed to obtain a better method for immediate FP.ResultsNo significant differences in IVM and survival rates were observed among the three groups. Nevertheless, GV-VI oocytes exhibited inferior quality, including abnormal spindle arrangement, mitochondrial dysfunction, and early apoptosis, compared to MII-VI and CON oocytes. Oocyte vitrification at the GV stage impacted maternal mRNA degradation during IVM. In addition, the GV-VI group demonstrated significantly lower embryonic developmental competence relative to the MII-VI group. RNA sequencing of 2-cell stage embryos revealed abnormal minor zygotic genome activation in the GV-VI group.ConclusionVitrification at the GV stage compromised oocyte quality and reduced developmental competence. Consequently, compared to the GV stage, oocyte vitrification at the MII stage after IVM is more suitable for patients who require immediate FP.

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Maturation conditions, post-ovulatory age, medium pH, and ER stress affect [Ca2+]i oscillation patterns in mouse oocytes

Cited by 6 publications

References 49 publications

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

In vivo and in vitro postovulatory aging: when time works against oocyte quality?

Melatonin loaded PLGA nanoparticles effectively ameliorate the in vitro maturation of deteriorated oocytes and the cryoprotective abilities during vitrification process

Effects of meiotic stage-specific oocyte vitrification on mouse oocyte quality and developmental competence

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