Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

Veeck, Lucinda L.; Wortham, J.W. Edward; Witmyer, J.; Sandow, Bruce A.; Acosta, Anı́bal A.; García, Jairo E.; Jones, George Fenwick; Jones, Howard W.

doi:10.1016/s0015-0282(16)47052-4

Cited by 362 publications

(85 citation statements)

References 18 publications

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“…Eighteen years later, the first in vitro maturation (IVM) baby came into this world [30]. It is estimated that there had been over 1300 IVM babies delivered worldwide till 2009 [6].…”

Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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Purpose Studies in bovine and porcine have indicated that melatonin (MT) could induce meiotic maturation of immature oocytes in vitro. The object of the current study was to investigate if MT could ameliorate human oocytes maturation during rescue in vitro maturation (IVM). Methods Two hundred seventy eight germinal vesicle (GV) oocytes and 451 (MI) metaphase I oocytes were vitrified, thawed and then matured in vitro. All the oocytes were randomly allocated into six groups in which the oocytes were cultured in medium supplemented with different concentrations of MT (0, 10 −2 , 1, 10 2 , 10 4 , 10 6 nM) and nuclear maturation was evaluated at 6 h, 12 h, 18 h, 24 h and 48 h of culture. Results The optimal MT concentration for both GV and MI oocytes was 1 nM. At 24 h of culture, nuclear maturation rate of MI oocytes cultured in 1 nM MT medium was significantly higher than other groups (P<0.05); Nuclear maturation rate of GV oocytes cultured in 1 nM MT medium was also significantly higher than the control group (P<0.05). On the other hand, decreased nuclear maturation rate was observed in the high MT concentration group (10 6 nM). Conclusions The current study demonstrated that low concentration of exogenous MT could ameliorate nuclear maturation of human oocyte during rescue IVM, while high concentration of MT presented negative effects.

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“…Eighteen years later, the first in vitro maturation (IVM) baby came into this world [30]. It is estimated that there had been over 1300 IVM babies delivered worldwide till 2009 [6].…”

Section: Introductionmentioning

confidence: 99%

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Wei

Zhang

Xie

et al. 2013

J Assist Reprod Genet

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“…For these calculations we did not consider oocytes classified as intermediate and the "gold standard" was microscopic analysis of the oocytes as described previously (10,11).…”

Section: Discussionmentioning

confidence: 99%

“…The corona-cumulus-oocyte complexes were first identified under a stereomicroscope and then evaluated for maturity under an inverted photomicroscope and the oocytes were classified as immature, intermediate and mature according to the criteria of Testart et al (10) and Veeck et al (11). Of the total of 107 oocytes retrieved, 32 of them were mature, 37 were intermediate and 38 were immature.…”

Section: Methodsmentioning

confidence: 99%

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Costa

Mendes

Ferriani

et al. 2004

Braz J Med Biol Res

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The objective of the present study was to examine the association between follicular fluid (FF) steroid concentration and oocyte maturity and fertilization rates. Seventeen infertile patients were submitted to ovulation induction with urinary human follicle-stimulating hormone, human menopausal gonadotropin and human chorionic gonadotropin (hCG). A total of 107 follicles were aspirated after hCG administration, the oocytes were analyzed for maturity and 81 of them were incubated and inseminated in vitro. Progesterone, estradiol (E2), estrone, androstenedione, and testosterone were measured in the FF. E2 and testosterone levels were significantly higher in FF containing immature oocytes (median = 618.2 and 16 ng/ml, respectively) than in FF containing mature oocytes (median = 368 and 5.7 ng/ml, respectively; P < 0.05). Progesterone, androstenedione and estrone levels were not significantly different between mature and immature oocytes. The application of the receiver-operating characteristic curve statistical approach to determine the best cut-off point for the discrimination between mature and immature oocytes indicated levels of 505.8 ng/ml for E2 (81.0% sensitivity and 81.8% specificity) and of 10.4 ng/ ml for testosterone (90.9% sensitivity and 82.4% specificity). Follicular diameter was associated negatively with E2 and testosterone levels in FF. There was a significant increase in progesterone/testosterone, progesterone/E2 and E2/testosterone ratios in FF containing mature oocytes, suggesting a reduction in conversion of C21 to C19, but not in aromatase activity. The overall fertility rate was 61% but there was no correlation between the steroid levels or their ratios and the fertilization rates. E2 and testosterone levels in FF may be used as a predictive parameter of oocyte maturity, but not for the in vitro fertilization rate.

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“…Cette transformation, ou « maturation ovocytaire», est rapide (environ 30 h dans l'espèce humaine) et survient normalement entre la décharge hypophysaire de l'hormone ovulante LH (ou l'administration de hCG) et la rupture du follicule mûr. Elle peut aussi survenir in vitro quand l'ovocyte I préovulatoire est artificiellement extrait de son follicule [10], comme cela arrive en fivète pour certains ovocytes recueillis immatures [11]. De tels ovules sont fécondables et aptes au développement (bien que dans des proportions réduites par rapport aux gamètes recueillis mûrs), mais ne représentent pas une source significative d'ovules humains.…”

Section: De L'ovocyte à L'ovuleunclassified

Des ovules en abondance ?

Testart

2004

Med Sci (Paris)

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Maturation and fertilization of morphologically immature human oocytes in a program of in vitro fertilization

Cited by 362 publications

References 18 publications

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Supplementation with low concentrations of melatonin improves nuclear maturation of human oocytes in vitro

Estradiol and testosterone concentrations in follicular fluid as criteria to discriminate between mature and immature oocytes

Des ovules en abondance ?

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