Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

Bottini, Massimo; Mébarek, Saida; Anderson, Karen; Strzelecka‐Kiliszek, Agnieszka; Bożycki, Łukasz; Simão, Adenilso da Silva; Bolean, Maytê; Ciancaglini, Pietro; Pikula, Joanna Bandorowicz; Pikula, Sławomir; Magne, David; Volkmann, Niels; Hanein, Dorit; Millán, José Luis; Buchet, René

doi:10.1016/j.bbagen.2017.11.005

Cited by 143 publications

(171 citation statements)

References 226 publications

(311 reference statements)

Supporting

Mentioning

154

Contrasting

Order By: Relevance

“…Biomineralization induced by MVs is a complex process in which Ca 2+ and P i accumulate inside MVs and induce nucleation and formation of apatitic minerals [5, 7]. Previously, we have characterized the incorporation of TNAP into proteoliposomes made of DPPC:Chol, DPPC:SM, or DPPC:Chol:SM by differential scanning calorimetry (DSC) [30].…”

Section: Resultsmentioning

confidence: 99%

“…Tissue-nonspecific alkaline phosphatase (TNAP, E.C. 3.1.3.1) [4, 5] is a glycosylphosphatidylinositol (GPI)-anchored protein present on the plasma membrane of osteoblasts and chondrocytes, and also on the surface of MVs [6]. TNAP provides P i needed for apatite crystallization by hydrolyzing phosphomonoesters [7].…”

Section: Introductionmentioning

confidence: 99%

“…TNAP provides P i needed for apatite crystallization by hydrolyzing phosphomonoesters [7]. Among various substrates, TNAP catalyzes the hydrolysis of pyrophosphate (PP i ), a mineralization inhibitor [8], thus facilitating the formation of apatite crystals [5, 9–11]. Adequate local concentrations of extracellular PP i , originating from the hydrolysis of sodium adenosine-5′-triphosphate (ATP) by nucleotide pyrophosphatases/phosphodiesterases (NPPs) or from transport of intracellular PP i by the action of progressive ankylosis protein (ANK), and of extracellular P i originating from the hydrolysis of PP i or of other phosphomonoesters by TNAP are essential to allow normal mineralization [5, 12–14].…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Simão¹,

Bolean²,

Favarin³

et al. 2018

J Bone Miner Metab

Self Cite

View full text Add to dashboard Cite

Tissue-nonspecific alkaline phosphatase (TNAP), a glycosylphosphatidylinositol-anchored ectoenzyme present on the membrane of matrix vesicles (MVs), hydrolyzes the mineralization inhibitor inorganic pyrophosphate as well as ATP to generate the inorganic phosphate needed for apatite formation. Herein, we used proteoliposomes harboring TNAP as MV biomimetics with or without nucleators of mineral formation (amorphous calcium phosphate and complexes with phosphatidylserine) to assess the role of the MVs’ membrane lipid composition on TNAP activity by means of turbidity assay and FTIR analysis. We found that TNAP-proteoliposomes have the ability to induce mineralization even in the absence of mineral nucleators. We also found that the addition of cholesterol or sphingomyelin to TNAP-proteoliposomes composed of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine reduced the ability of TNAP to induce biomineralization. Our results suggest that the lipid microenvironment is essential for the induction and propagation of minerals mediated by TNAP.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Simão¹,

Bolean²,

Favarin³

et al. 2018

J Bone Miner Metab

Self Cite

View full text Add to dashboard Cite

show abstract

“…The structure and function of MVs in skeletal and vascular mineralization has recently been reviewed by several authors . The biogenesis of these vesicles may occur through multiple proposed mechanisms; however, the most prevalent theories include polarized budding from the parental cell membrane, or from microvilli on the cell surface, as demonstrated in hypertrophic chondrocytes and SaOS‐2 osteoblast‐like cells .…”

Section: The Phospho1 Mineralization Mechanism: Matrix Vesicle–mediatmentioning

confidence: 99%

How To Build a Bone: PHOSPHO1, Biomineralization, and Beyond

et al. 2019

Self Cite

View full text Add to dashboard Cite

Since its characterization two decades ago, the phosphatase PHOSPHO1 has been the subject of an increasing focus of research. This work has elucidated PHOSPHO1's central role in the biomineralization of bone and other hard tissues, but has also implicated the enzyme in other biological processes in health and disease. During mineralization PHOSPHO1 liberates inorganic phosphate (P i ) to be incorporated into the mineral phase through hydrolysis of its substrates phosphocholine (PCho) and phosphoethanolamine (PEA). Localization of PHOSPHO1 within matrix vesicles allows accumulation of P i within a protected environment where mineral crystals may nucleate and subsequently invade the organic collagenous scaffold. Here, we examine the evidence for this process, first discussing the discovery and characterization of PHOSPHO1, before considering experimental evidence for its canonical role in matrix vesicle–mediated biomineralization. We also contemplate roles for PHOSPHO1 in disorders of dysregulated mineralization such as vascular calcification, along with emerging evidence of its activity in other systems including choline synthesis and homeostasis, and energy metabolism. © 2019 The Authors. JBMR Plus published by Wiley Periodicals, Inc. on behalf of American Society for Bone and Mineral Research.

show abstract

“…Biomineralization is a complex process in which interactions between inorganic and organic materials determine the speed of formation and the size of deposited crystals. Crystal formation inside matrix vesicles (MVs) is the main mechanism currently thought to explain physiological mineralization (Bottini et al, 2018). However, MVs are not sufficiently abundant to explain mineralization in bone (Murshed & McKee, 2010), and crystal deposition has also been observed in absence of MVs (Landis, Hodgens, Arena, Song, & McEwen, 1996).…”

Section: Mechanisms Of Calcificationmentioning

confidence: 99%

The role of the gasotransmitter hydrogen sulfide in pathological calcification

Castelblanco

Nasi

et al. 2019

British J Pharmacology

View full text Add to dashboard Cite

Calcification is the deposition of minerals, mainly hydroxyapatite, inside the cell or in the extracellular matrix. Physiological calcification is central for many aspects of development including skeletal and tooth growth; conversely, pathological mineralization occurs in soft tissues and is significantly associated with malfunction and impairment of the tissue where it is located. Various mechanisms have been proposed to explain calcification. However, this research area lacks a more integrative, systemic, and global perspective that could explain both physiological and pathological processes. In this review, we propose such an integrated explanation. Hydrogen sulfide (H2S) is a newly recognized multifunctional gasotransmitters and tis actions have been studied in different physiological and pathological contexts, but little is known about its potential role on calcification. Interestingly, we found that H2S promotes calcification under physiological conditions and has an inhibitory effect on pathological processes. This makes H2S a potential therapy for diseases related to pathological calcification. Linked Articles This article is part of a themed section on Hydrogen Sulfide in Biology & Medicine. To view the other articles in this section visit http://onlinelibrary.wiley.com/doi/10.1111/bph.v177.4/issuetoc

show abstract

Matrix vesicles from chondrocytes and osteoblasts: Their biogenesis, properties, functions and biomimetic models

Abstract: MVs have been extensively investigated owing to their roles in skeletal and ectopic mineralization. MVs serve as a model system for lipid raft structures, and for the mechanisms of genesis and release of extracellular vesicles.

Cited by 143 publications

References 226 publications

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

Lipid microenvironment affects the ability of proteoliposomes harboring TNAP to induce mineralization without nucleators

How To Build a Bone: PHOSPHO1, Biomineralization, and Beyond

The role of the gasotransmitter hydrogen sulfide in pathological calcification

Contact Info

Product

Resources

About