Matrix replenishing by BMSCs is beneficial for osteoarthritic temporomandibular joint cartilage

Zhang, Mian; Yang, Hongxu; Lü, Lei; Wan, Xianghong; Zhang, Jing; Zhang, Hongyun; Liu, Xiao Dong; Huang, Xiaofeng; Xiao, Guozhi; Wang, Meiqing

doi:10.1016/j.joca.2017.05.007

Cited by 34 publications

(77 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…We attribute this thinning of the deep zone cartilage to the lower differentiation activity of the deep zone chondrocytes, which differs from that observed in the UAC model. As we demonstrated previously, UAC stimulates cellular death and promotes the deep zone chondrocytes to undergo terminal differentiation, leading to a reduction in the deep zone cartilage thickness (Zhang et al, ). In contrast, there were few TUNEL‐positive cells in the BAE cartilage throughout the observation period.…”

Section: Discussionsupporting

confidence: 55%

“…The deep zone cells, however, are maturely differentiated. We previously reported that the deep zone chondrocytes respond to the UAC in vivo and flow fluid shear stress (FFSS) in vitro by enhancing terminal differentiation (Zhang et al, ). In contrast, in the present study, BAE in vivo and CTS in vitro did not induce the terminal differentiation of deep zone chondrocytes.…”

Section: Discussionmentioning

confidence: 99%

“…Cells from the superficial and deep zones of the condylar cartilage were isolated separately as we previously reported (Zhang et al, ) from 3‐week‐old female Sprague–Dawley rats under the microscope through enzymatic digestion for 20 min using 0.25% parenzyme (HyClone) and then incubated with 0.2% type II collagenase (Gibco) in DMEM (HyClone) for 2.5 hr. The isolated cells were collected under centrifugation at 120 g for 5 min and then resuspended in DMEM supplemented with 10% (v/v) FBS (Bioind), 50 mg/ml streptomycin, and 50 units/ml penicillin (HyClone).…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle

et al. 2019

View full text Add to dashboard Cite

Objective We aimed to develop a mouse model predominating in a proliferative response in the articular cartilage of the temporomandibular joints. Materials and Methods Bilateral anterior elevation of occlusion was developed by installing metal tubes onto the incisors of mice with edge‐to‐edge relation to prevent tooth wear, leading to an increase in the vertical height of the dental occlusion with time. Morphological changes and expression changes in Cyclin D1, Aggrecan, and type II and type X collagen in the mandibular condylar cartilage were detected. In addition, cells were isolated from the mandibular condylar cartilage and exposed to cyclic tensile strain (CTS). Results Compared with age‐matched controls, the tooth length was longer at 3 weeks, 7 weeks, and 11 weeks in BAE mice (p < 0.05), with increased condylar cartilage thickness, matrix amount, and cell number (p < 0.05). Compared with the deep zone cells, CTS stimulated the superficial zone cells to express a higher level of proliferating cell nuclear antigen, Cyclin D1, Aggrecan, and type II collagen but a lower level of type X collagen and alkaline phosphatase. Conclusion Bilateral anterior elevation stimulated the proliferative response in the mandibular condylar cartilage, offering a new therapeutic strategy for cartilage degeneration.

show abstract

Section: Discussionsupporting

confidence: 55%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Experimental animal models have shown that aberrant biomechanical stimulation from dental occlusion plays an important role in TMJ OA development . To imitate the aberrant biomechanical condition, we developed an in vitro biomechanical cellular model by exposing ATDC5 cells to the fluid flow shear stress (FFSS) stimulation, termed FFSS in vitro model, in which there was promoted differentiation in addition to cellular death . In the past few years, we reported a rodent TMJ OA model developed in our group by applying a pair of incisor prosthesis with a unilateral anterior crossbite (UAC) relation.…”

Section: Introductionmentioning

confidence: 99%

Calcium‐/calmodulin‐dependent protein kinase II in occlusion‐induced degenerative cartilage of rat mandibular condyle

Liu

Yang

Wan

et al. 2018

J of Oral Rehabilitation

Self Cite

View full text Add to dashboard Cite

Activated calcium-/calmodulin-dependent protein kinaseII (CaMKII) is important to promote chondrocytes from proliferative to pre-hypertrophic state, which probably plays a role in osteoarthritis (OA), a widespread degeneration disease with enhanced aberrant chondrocyte differentiation. Our aim was to detect the role of CaMKII, and its relationship with the feedback loop of Indian hedgehog (Ihh) and Parathyroid-related peptide (PTHrP) in the temporomandibular joints (TMJs) OA. KN93, the competitive inhibitor of CaMKII, was added to the culture medium in vitro and was locally injected to rats TMJs (n = 54, female) every other day for 4 weeks from the beginning of the 5th and 9th week after installing of unilateral anterior crossbite (UAC), termed as 4 wk+4 wk and 8 wk+4 wk, accordingly. The RNA expression of CaMKII α (1.49 ± 0.09), CaMKII β (3.36 ± 0.20), Ihh (1.88 ± 0.06) and PTHrP (1.87 ± 0.12) was all enhanced, especially at 24 dyn/cm in vitro (all P < .05), accompanied with downregulated expression of cartilage matrix, but upregulated markers of chondrocytes differentiation (all P < 0.05). Similarity was observed in the 4 wk+4 wk group in vivo. In the 8 wk+4 wk group, UAC upregulated the RNA expression of CaMKII α (1.81 ± 0.24), CaMKII β (1.36 ± 0.07) and Ihh (1.70 ± 0.21), however, down-regulated PTHrP (0.53 ± 0.04) (all P < .05), in consonance with the protein expression. All these changes were attenuated by KN93 (all P < .05). In conclusion, CaMKII took a role, via Ihh and PTHrP pathways, in promoting biomechanically induced TMJ chondrocytes differentiation, the initiation issue of UAC stimulated osteoarthritic changes in rodent TMJs. Inhibiting CaMKII is helpful to rescue the biomechanically stimulated cartilage degradation and prospective to be a target treatment of OA.

show abstract

“…Significant mineralisation was exhibited at 12 and 20 weeks after UAC operation, which was displayed as osteochondral interface stiffening. 16,19,20 As a result, we used the 20-week group for the PBL mRNA microarray detections and the 12-and 20-week groups to detect the TMJ and alveolar tissues for verification.…”

Section: Animals and Uac Operationmentioning

confidence: 99%

Molecular changes in peripheral blood involving osteoarthritic joint remodelling

Zhang

Liu

et al. 2019

J of Oral Rehabilitation

View full text Add to dashboard Cite

Biomarkers of temporomandibular joint (TMJ) osteoarthritis (OA) remain unknown. The objective was to detect whether molecular biomarkers from peripheral blood leucocytes (PBLs) engage in TMJ OA lesions. Thirty‐four six‐week‐old Sprague Dawley rats were used. The top upregulated gene ontology categories and gene‐fold changes in PBLs were detected by a microarray analysis comparing rats that received 20‐week unilateral anterior crossbite (UAC) treatment with age‐matched controls (n = 4). Twenty weeks of UAC treatment had been reported to induce TMJ OA‐like lesions. The other twenty‐four rats were randomly placed in the UAC and control groups at 12‐ and 20‐week time points (n = 6). The mRNA expression levels of the selected biomarkers derived from the microarray analysis and their protein expression in the alveolar bone and TMJ were detected. The microarray analysis indicated that the three most highly involved genes in PBLs were Egr1, Ephx1 and Il10, which were confirmed by real‐time PCR detection. The increased protein expression levels of the three detected molecules were demonstrated in cartilage and subchondral bone (P < 0.05), and increased levels of EPHX1 were reported in discs (P < 0.05); however, increased levels were not present in the alveolar bone. Immunohistochemistry revealed the increased distribution of EGR1‐positive, EXPH1‐positive and IL10‐positive cells predominantly in the osteochondral interface, with EXPH1 also present in TMJ discs. In conclusion, the increased mRNA expression of Egr1, Ephx1 and Il10 in PBLs may serve as potential biomarkers for developed osteoarthritic lesions relating to osteochondral interface hardness changes induced by dental biomechanical stimulation.

show abstract

Matrix replenishing by BMSCs is beneficial for osteoarthritic temporomandibular joint cartilage

Abstract: Scavenging the degraded matrix and replenishing the fibrosis-developmental matrix are the primary requirements for the repair of OA cartilage.

Cited by 34 publications

References 30 publications

Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle

Bilateral anterior elevation prosthesis boosts chondrocytes proliferation in mice mandibular condyle

Calcium‐/calmodulin‐dependent protein kinase II in occlusion‐induced degenerative cartilage of rat mandibular condyle

Molecular changes in peripheral blood involving osteoarthritic joint remodelling

Contact Info

Product

Resources

About