Matrix remodeling in healing rabbit Achilles tendon

Reddy, G. Kesava; Stehno‐Bittel, Lisa; Enwemeka, Chukuka S.

doi:10.1046/j.1524-475x.1999.00518.x

Cited by 67 publications

(57 citation statements)

References 31 publications

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“…Organization was decreased initially after surgery but increased over healing time in the control group, as has been seen in several previous studies [23,29,34]. This phenomenon was not seen in the early group, which when compared to the control had increased fiber organization at 1 week postsurgery that did not change over time.…”

Section: Discussionsupporting

confidence: 79%

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

Connizzo

Yannascoli

Tucker

et al. 2014

Clinical Orthopaedics &Amp; Related Research

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Background Current clinical treatment after tendon repairs often includes prescribing NSAIDs to limit pain and inflammation. The negative influence of NSAIDs on bone repair is well documented, but their effects on tendon healing are less clear. While NSAIDs may be detrimental to early tendon healing, some evidence suggests that they may improve healing if administered later in the repair process.Questions/purposes We asked whether the biomechanical and histologic effects of systemic ibuprofen administration on tendon healing are influenced by either immediate or delayed drug administration. Methods After bilateral supraspinatus detachment and repair surgeries, rats were divided into groups and given ibuprofen orally for either Days 0 to 7 (early) or Days 8 to 14 (delayed) after surgery; a control group did not receive ibuprofen. Healing was evaluated at 1, 2, and 4 weeks postsurgery through biomechanical testing and histologic assessment. Results Biomechanical evaluation resulted in decreased stiffness and modulus at 4 weeks postsurgery for early ibuprofen delivery (mean ± SD [95% CI]: 10.8 ± 6.4 N/mm [6.7-14.8] and 8.9 ± 5.9 MPa [5.4-12.3]) when compared to control repair .2]) (p = 0.003 and 0.013); however, there were no differences between the delayed ibuprofen group (18.1 ± 7.4 N/mm [14.2-22.1] and 11.5 ± 5.6 MPa [8.2-14.9]) and the control group. Histology confirmed mechanical results with reduced fiber reorganization over time in the early ibuprofen group.

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Section: Discussionsupporting

confidence: 79%

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

Connizzo

Yannascoli

Tucker

et al. 2014

Clinical Orthopaedics &Amp; Related Research

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“…Since we noted that after 12 weeks only 60% of the tensile strength was reached and that there were still significant differences in collagen type I and type III ratios, it is evident that the long-term outcome cannot be confidently assessed after a period of only 6 weeks. For assessment of matrix remodeling in the healing rabbit AT as carried out on day 15 by Reddy et al (17), such early time points make more sense.…”

Section: Discussionmentioning

confidence: 99%

Impact of combinatory growth factor application on rabbit Achilles tendon injury with operative versus conservative treatment: A pilot study

Konerding¹

2009

Int J Mol Med

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Abstract. Acute Achilles tendon (AT) rupture is a common injury with a comparatively high complication rate. Presently, surgical treatments compete with nonoperative treatment modalities. The aim of this study was to elucidate the possible beneficial effects of short-term combinatory application of growth factors on tendon healing during operative or conservative treatment. In this controlled laboratory study, the left ATs of 40 adult New Zealand White rabbits were transected and either sutured or treated conservatively. Half of the animals from each treatment modality group repetitively received a mixture of VEGF165, bFGF, and rPDGF which was administered peritendineally. The left legs were immobilized with external fixateurs for 6 weeks. The ATs were harvested 3 months after intervention. Tensile strength tests revealed no significant differences between operative and conservative treatments. Compared to the normal right ATs, 60% of the average breaking strength was reached 3 months after surgery. Growth factor application did not result in significant improvements. Only a tendency towards higher blood vessel densities was noted in the groups treated with the factors. Collagen type I/III ratios also displayed no significant differences. This study indicates that there is no difference in the biomechanical outcome of conservative versus operative AT rupture treatment and only a marginal impact of short-term combinatory growth and angiogenesis factor application.

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“…The atria and residual vessels were removed. The intact left and right ventricles were homogenized in D-PBS, and the tissue hydroxyproline and protein content were determined by using colorimetric methods (22,23).…”

Section: Confocal Microscopic Colocalization Ofmentioning

confidence: 99%

Heart regeneration in adult MRL mice

Leferovich

Bedelbaeva

Samulewicz

et al. 2001

Proc. Natl. Acad. Sci. U.S.A.

234

190

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The reaction of cardiac tissue to acute injury involves interacting cascades of cellular and molecular responses that encompass inflammation, hormonal signaling, extracellular matrix remodeling, and compensatory adaptation of myocytes. Myocardial regeneration is observed in amphibians, whereas scar formation characterizes cardiac ventricular wound healing in a variety of mammalian injury models. We have previously shown that the MRL mouse strain has an extraordinary capacity to heal surgical wounds, a complex trait that maps to at least seven genetic loci. Here, we extend these studies to cardiac wounds and demonstrate that a severe transmural, cryogenically induced infarction of the right ventricle heals extensively within 60 days, with the restoration of normal myocardium and function. Scarring is markedly reduced in MRL mice compared with C57BL͞6 mice, consistent with both the reduced hydroxyproline levels seen after injury and an elevated cardiomyocyte mitotic index of 10 -20% for the MRL compared with 1-3% for the C57BL͞6. The myocardial response to injury observed in these mice resembles the regenerative process seen in amphibians.

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Matrix remodeling in healing rabbit Achilles tendon

Cited by 67 publications

References 31 publications

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

The Detrimental Effects of Systemic Ibuprofen Delivery on Tendon Healing Are Time-Dependent

Impact of combinatory growth factor application on rabbit Achilles tendon injury with operative versus conservative treatment: A pilot study

Heart regeneration in adult MRL mice

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